Presence of Arcobacter species in pet cats and dogs in the Czech Republic

Abstract

ABSTRACT:This study was conducted to evaluate the occurrence of the genus Arcobacter in cats and dogs in the Czech Republic. These animals may be carriers of the bacteria and potential sources of human infection. Oral smears were collected from animals using smear swabs and brushes. Based on previous studies, commercially available DNA kits were used for DNA isolation. Samples were analysed using polymerase chain reaction (PCR) and evaluated using gel electrophoresis. Overall, 178 oral smears were tested, of which 108 were from dogs and 70 were from cats. Out of all smears, five were positive, of which four samples were from dogs and one from a cat. In all five positive cases, PCR confirmed the presence of Arcobacter butzleri. In follow-up sampling, the presence of Arcobacter butzleri was demonstrated in two samples from a dog.

Prevalence, Comparison of Diagnostic Methods, Antibiogram, and Genotyping of Arcobacter spp. in Diarrheal Cases Referring to Clinical Centers in Iran

“…Discrepancies in isolation rates may be due to the use of different isolation methods. Some of the factors influencing isolation rate are as follows: the sensitivity of isolation methods, test conditions (optimum incubation time, temperature required, type of culture medium, and atmosphere), study population, study area, diet, the degree of exposure to natural reservoirs of Arcobacter species, the immunity level of the studied population, living conditions, drinking water status, nutritional habits especially nutrition containing poultry, and the relationship with animals and the environment such as exposure to pets [25][26][27][28] .…”