Presence of blaCTX-M antibiotic resistance gene in Lactobacillus spp. isolated from Hirschsprung diseased infants with stoma

Abstract

Introduction: Although antibiotics have revolutionized health care by saving lives, the evolution of both pathogenic and commensal antibiotic-resistant bacteria are emerging as a threat in the health sector. As for Lactobacillus spp., it is usually a non-pathogenic bacteria. However, it can cause infection in immunocompromised condition. In this study, Lactobacillus spp. has been isolated from the faeces of infants with Hirschsprung disease (HD), which is congenital aganglionosis of intestine, where surgical approach and antibiotics are frequently used as medical intervention. The aim of this study is to assess the antibiotic resistance pattern and determine the presence of resistance genes, if any, in Lactobacillus spp. isolated from HD infants with ileostomy.
 Methodology: Six Lactobacillus spp. were isolated from faeces of six HD infants and confirmed using both conventional and molecular methods. Antibiotic resistance pattern was checked through disc diffusion method and was further investigated for the presence of antibiotic resistance genes (blaTEM, blaCTX-M, blaOXA-2, blaIMP, blaVIM-2, blaNDM-1 and mcr-1).
 Results: Antibiotic susceptibility of the isolates showed high level of resistance towards cephalosporins, oxacillin, aztreonam, meropenem and polymyxin group. However, four of the isolates showed the presence of blaCTX-M gene after PCR amplification.
 Conclusions: To our knowledge, this is the first report on the presence of antibiotic resistance gene blaCTX-M in Lactobacillus spp. and this presence may pose a serious threat in treatment regimen. As not much is known regarding the presence of blaCTX-M in Lactobacillus spp., this finding may provide new light to research on antibiotic resistance in gut microflora.

Antibiotic Resistance in Potential Probiotic Lactobacillary Strains of Fermented Foods and Human Origin From Nigeria

“…Complete (100%) resistance was observed towards all three cephalosporins (cefoxitin, cefmetazole and ceftazidime) tested in this study, supporting the above view. Our results are further supported by those of others [77,71,78]. In the case of Charteris et al…”

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Alarming Antibiotic Resistance of Lactobacilli Isolated from Probiotic Preparations and Dietary Supplements

“…β-Lactamases represent the main mechanism of bacterial resistance to β-lactam antibiotics. Some extended-spectrum beta-lactamases (ESBLs) have been detected in clinical isolates of lactobacilli : OXA-48 [ 33 ], bla TEM, bla OXA-1 , bla SHV [ 35 ], and bla CTX-M [ 36 ]. In our previous study, we revealed a wide distribution of the bla TEM gene in Lactobacillus isolates from different sources and detected genes bla SHV and bla OXA-1 in some of them [ 29 ].…”

Combined Anti-Bacterial Actions of Lincomycin and Freshly Prepared Silver Nanoparticles: Overcoming the Resistance to Antibiotics and Enhancement of the Bioactivity

“…Genic expression levels of the two antibiotic resistance genes, β-lactamase producing ( Bla ) in B. cereus [ 24 ], and ( Bla CTX-M-15 ) in the P. mirabilis were examined [ 25 , 26 ]. First, the cells’ total RNA (treated and untreated with AgNPs) was extracted using an RNA extraction kit (TransGen Biotech, Zol UpPlus RNA Kit) according to the manufacturer’s instructions.…”