Presence of Chemosensitive Sif Cells in the Rat Sympathetic Ganglia: A Biochemical, Immunocytochemical and Pharmacological Study

Dalmaz, Y.; Borghini, N.; Péquignot, Jean-Marc; Peyrin, L

doi:10.1007/978-1-4615-2966-8_55

Cited by 11 publications

(5 citation statements)

References 10 publications

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“…TASK-3 immunoreactivity in paraganglionic cells of both sympathetic and glossopharyngeal/vagal paraganglia appears to indicate that sympathetic paraganglionic cells, as well as chief cells of the carotid and aortic bodies, have an oxygen-sensing property. The oxygen-sensing property of paraganglionic cells in the superior cervical ganglion has been reported on the basis of dopamine turnover (Dalmaz et al 1990). Furthermore, paraganglionic cells expressed hypoxia-inducible factor (HIF) 2a and neuroblastoma cells showed elevated expression of HIF1a and/or HIF2a under hypoxic conditions in fetal mice (Jogi et al 2002).…”

Section: Resultsmentioning

confidence: 99%

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Heterogeneous expression of TASK-3 and TRAAK in rat paraganglionic cells

Yamamoto

Taniguchi

2003

Histochemistry and Cell Biology

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In the present study, we investigated the immunohistochemical localization of the two-pore K+ channels, TASK-3 and TRAAK, in paraganglionic cells within the superior cervical ganglion, stellate ganglion, and aortic body in comparison with membrane channels in chief cells of the carotid body. TASK-3 immunoreactivity was observed in the paraganglionic cells in all tissues examined. TRAAK immunoreactivity was observed in the chief cells of the aortic body as well as these of the carotid body, but not in the paraganglionic cells in the sympathetic (superior cervical and stellate) ganglia. Our findings indicate that sympathetic paraganglionic cells and glossopharyngeal/vagal paraganglionic cells were different from each other in the expression patterns of TASK-3 and TRAAK to result in the different chemoreception properties of sympathetic paraganglionic cells from those of chief cells of the aortic and carotid bodies.

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Section: Resultsmentioning

confidence: 99%

“…They are mainly involved in the regulation of ganglionic cells as interneurons and paracrine cells rather than in chemoreception (Kondo 1977). In addition to these functions, it has been reported that SIF cells in the rat superior cervical ganglion respond to hypoxia with an increase in dopamine turnover (Dalmaz et al 1990).…”

Section: Introductionmentioning

confidence: 99%

Heterogeneous expression of TASK-3 and TRAAK in rat paraganglionic cells

Yamamoto

Taniguchi

2003

Histochemistry and Cell Biology

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“…Their functional significance is still not fully resolved, and they have been interpreted as interneurons, endo-/paracrine cells, and arterial chemoreceptors (Kondo 1977;Matthews 1989). SIF cells of the rat superior cervical ganglion (SCG) respond to hypoxia with an increased turnover of dopamine (Dalmaz et al 1993;Borghini et al 1994). The oxygen-sensing mechanism in these cells has received little attention as yet.…”

Section: Introductionmentioning

confidence: 99%

Cytochrome b 558 and hydrogen peroxide production in small intensely fluorescent cells of sympathetic ganglia

Kummer

Acker

1997

Histochemistry and Cell Biology

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Small intensely fluorescent (SIF) cells are paraganglionic cells derived from sympathicoblasts which may serve as interneurons, endo-/paracrine cells or arterial chemoreceptors within sympathetic ganglia. Like paraganglionic cells of other locations, e.g., carotid body glomus cells, they are responsive to hypoxia. Recent studies on glomus cells and other hypoxia-sensing cells suggested the involvement of a b558-type cytochrome and intracellular generation of H2O2 in the process of oxygen sensing. In the present study, we demonstrate the occurrence of the small subunit of cytochrome b558, p22phox, in SIF cells of guinea-pig sympathetic ganglia by immunohistochemistry using two different antisera. H2O2 production was monitored in explanted intact superior cervical ganglia of 2-day-old rats by confocal laser scanning analysis of rhodamine 123 fluorescence generated due to oxidation of dihydrorhodamine 123 by H2O2. Using this technique, SIF cell clusters appeared as sites of highest H2O2 production within the ganglia. Thus, SIF cells exhibit two key features of an oxidase system generating reactive oxygen species. This may be involved in the proposed chain of events in oxygen sensing, but alternative cellular functions of this system have also to be considered.

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“…However, after CH a few groups of cells resembling small intensely fluorescent cells were immunopositive for Cx43 (not shown). Previous studies have shown that small intensely fluorescent cells share multiple cytological features with carotid body type I cells (15).…”

Section: Effect Of Ch On Cx43 Immunoreactivity In Carotid Body and Pementioning

confidence: 97%

Chronic hypoxia upregulates connexin43 expression in rat carotid body and petrosal ganglion

Chen

Dinger

et al. 2002

Journal of Applied Physiology

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Recent studies have demonstrated that oxygen-sensitive type I cells in the carotid body express the gap junction-forming protein connexin43 (Cx43). In the present study, we examined the hypothesis that chronic exposure to hypoxia increases Cx43 expression in type I cells as well as in chemoafferent neurons in the petrosal ganglion. Immunocytochemical studies in tissues from normal rats revealed diffuse and granular Cx43-like immunoreactivity in the cytoplasm of type I cells and dense punctate spots of immunoreactive product at the margins of type I cells and near the borders of chemosensory cell lobules. Cx43-like immunoreactivity was not detectable in petrosal ganglion neurons from normal animals. After a 2-wk exposure to hypobaric (380 Torr) hypoxia, Cx43 immunostaining was substantially enhanced in and around type I cells. Moreover, chronic hypoxia elicited the expression of Cx43-like immunoreactivity in the cytoplasm of afferent neurons throughout the petrosal ganglion. Quantitative RT-PCR studies indicate that chronic hypoxia evokes a substantial increase in Cx43 mRNA levels in the carotid body, along with a marked elevation of Cx43 expression in the petrosal ganglion. Increased Cx43 expression and gap junction formation in type I cells and sensory neurons may contribute to carotid body adaptation during sustained stimulation in extreme physiological conditions.

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Presence of Chemosensitive Sif Cells in the Rat Sympathetic Ganglia: A Biochemical, Immunocytochemical and Pharmacological Study

Cited by 11 publications

References 10 publications

Heterogeneous expression of TASK-3 and TRAAK in rat paraganglionic cells

Heterogeneous expression of TASK-3 and TRAAK in rat paraganglionic cells

Cytochrome b 558 and hydrogen peroxide production in small intensely fluorescent cells of sympathetic ganglia

Chronic hypoxia upregulates connexin43 expression in rat carotid body and petrosal ganglion

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