Presence of chlorogenic acid during in vitro maturation protects porcine oocytes from the negative effects of heat stress

Nguyen, Thanh-Van; Kim, Lanh Thi; Somfai, T.; Otoi, Takeshige; Taniguchi, Masayasu; Kikuchi, Kazuhiro

doi:10.1111/asj.13302

Cited by 8 publications

(9 citation statements)

References 34 publications

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“…A protective effect of CGA on DNA integrity as well as on MMP was observed. These results are supported by the observation that CGA protected in vitro porcine oocytes from the negative effects of heat stress and from DNA damage [ 44 ]. In addition, since mitochondria generate energy to support motility, high percentage of sperm with high MMP represents an index of cellular activity, vitality, and integrity of mitochondrial cristae.…”

Section: Discussionmentioning

confidence: 53%

Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen–Thawed Protocol

et al. 2021

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The study evaluated the chlorogenic acid (CGA) antioxidant potential on oxidative stress (OS) induced in vitro in human spermatozoa and during cryopreservation procedure. Swim-up selected spermatozoa were treated with 100 µM CGA, 100 µM H2O2 to induce lipid peroxidation (LPO), and with both compounds and the effects on mitochondrial membrane potential (MMP) by JC-1, DNA integrity by acridine orange (AO), and sperm ultrastructure by transmission electron microscopy (TEM), were evaluated. CGA antioxidant activity was assessed by measuring malondialdehyde (MDA) and F2-isoprostanes (F2-IsoPs) in the media. The CGA protective activity and the immunolocalization of Phospho-AMPKα (Thr172) were explored in frozen-thawed sperm. CGA was not toxic for sperm motility, DNA integrity and MMP. The increase in MDA (p < 0.05) and F2-IsoPs (p < 0.001), DNA damage (p < 0.01) and low MMP (p < 0.01) levels after H2O2 treatment were reduced in presence of CGA as well as the percentage of broken plasma membranes (p < 0.01) and altered acrosomes (p < 0.01) detected by TEM. Treated frozen-thawed spermatozoa showed increased sperm motility (p < 0.01), DNA integrity (p < 0.01), MMP (p < 0.01), reduced MDA (p < 0.01) and increased sperm percentage with Phospho-AMPKα labelling in the head (p < 0.001). CGA can be used to supplement culture media during semen handling and cryopreservation where OS is exacerbated.

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Section: Discussionmentioning

confidence: 53%

Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen–Thawed Protocol

et al. 2021

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“…However, there were no differences in nuclear maturation and the proportion of oocytes with fragmented DNA between the antioxidant‐supplemented and control groups. In contrast, other studies have shown that supplementation with curcumin (Namula et al., 2020) and CGA (Nguyen et al., 2019) during maturation in the absence of cysteine increased nuclear maturation and protected oocytes from DNA fragmentation. The lack of positive effect of antioxidants supplementation on nuclear maturation and DNA damage in the oocytes may be partly due to adding 0.6 mM cysteine during maturation culture.…”

Section: Discussionmentioning

confidence: 76%

“…Chlorogenic acid (CGA) is a phenolic acid found mainly in coffee. CGA plays an essential role in protecting porcine oocytes from oxidative stress induced by heat stress and electroporation (Nguyen et al., 2018,2019). Our earlier study had demonstrated that application of 50 μM CGA improved maturation rates, fertilization and blastocyst formation and reduced the percentage of DNA fragmentation in porcine oocytes exposed to oxidative stress induced by H 2 O 2 during maturation culture (Nguyen et al., 2017).…”

Section: Introductionmentioning

confidence: 99%

Effects of individual or in‐combination antioxidant supplementation during in vitro maturation culture on the developmental competence and quality of porcine embryos

Thongkittidilok

Lin

et al. 2021

Reprod Domestic Animals

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The oocyte maturation process requires a high supply of energy, which generates reactive oxygen species (ROS), adversely affecting oocyte and embryo development.Balancing ROS by antioxidant supplementation is essential for maintaining oocyte maturation and embryonic quality in vitro. This study aimed to evaluate the impact of four antioxidants: β-mercaptoethanol (β-ME), chlorogenic acid (CGA), curcumin and sericin, when applied individually or in combinations, during oocyte maturation on development of porcine oocytes. Cumulus-oocyte complexes were collected, cultured in maturation medium supplemented with antioxidants for 44 hr and subsequently subjected to in vitro fertilization (IVF) and culture for 7 days. Combining all four (β-ME + CGA + curcumin + sericin) or three (β-ME + CGA + curcumin) antioxidants increased blastocyst formation rates. However, sericin supplementation alone, or in combination with β-ME or CGA, failed to improve blastocyst formation rates. The total cell numbers of blastocysts from the group supplemented with three antioxidants (β-ME + CGA + curcumin) were significantly higher than those from the other groups, except for the curcumin-supplement group. There were no differences in the maturation rates and proportions of oocytes with fragmented DNA between the antioxidant-supplemented and the non-supplemented control groups. In conclusion, supplementation with three antioxidants (β-ME + CGA + curcumin) during the maturation culture enhanced blastocyst formation and improved blastocyst quality.

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“… Namula et al (2018) suggested that CA supplementation improved certain sperm parameters, including viability, plasma membrane integrity, and motility, during boar sperm freezing. Nguyen et al (2019) found that CA supplementation during in vitro maturation could protect porcine oocytes from DNA damage induced by oxidative stress and improve their subsequent developmental competence after fertilization.…”

Section: Introductionmentioning

confidence: 99%

Chlorogenic Acid Ameliorates Damage Induced by Fluorene-9-Bisphenol in Porcine Sertoli Cells

et al. 2021

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4,4′-(9-Fluorenylidene) diphenol (BPFL, also known as BHPF and fluorene-9-bisphenol) is a novel bisphenol A substitute that is used in the plastics industry as an organic synthesis intermediate and is a potential endocrine disruptor. However, the deleterious effects of BPFL on porcine Sertoli cells (SCs) and the possible underlying mechanisms are still unclear. Chlorogenic acid (CA) is a free radical scavenger in the cellular antioxidant system that prevents oxidative damage and apoptosis. In the present research, we found that BPFL induced impairments in porcine SCs in a dose-dependent manner and that CA protected porcine SCs against BPFL exposure-induced impairments. Cell viability, proliferation and apoptosis assay results revealed that BPFL exposure could inhibit porcine SC proliferation and induce apoptosis, while CA supplementation ameliorated the effects of BPFL. Further analysis revealed that BPFL exposure induced oxidative stress, mitochondrial membrane potential dysfunction and DNA damage accumulation. Transcriptome analysis and further real-time quantitative PCR and Western blot results showed that BPFL exposure induced endoplasmic reticulum stress and apoptosis. Supplementation with CA dramatically ameliorated these phenotypes in BPFL-exposed porcine SCs. Overall, the present research reveals the possible underlying mechanisms by which BPFL exposure induced impairments and CA supplementation protected against these impairments in porcine SCs.

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Presence of chlorogenic acid during in vitro maturation protects porcine oocytes from the negative effects of heat stress

Cited by 8 publications

References 34 publications

Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen–Thawed Protocol

Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen–Thawed Protocol

Effects of individual or in‐combination antioxidant supplementation during in vitro maturation culture on the developmental competence and quality of porcine embryos

Chlorogenic Acid Ameliorates Damage Induced by Fluorene-9-Bisphenol in Porcine Sertoli Cells

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