Presence of Estrogen Receptors in the Hen Hypothalamus and Pituitary

Kawashima, Mitsuo; Kamiyoshi, Michiharu; Tanaka, Katuhide

doi:10.1210/endo-120-2-582

Cited by 38 publications

(24 citation statements)

References 39 publications

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“…The presence of an estrogen receptor in the human follicle-albeit limited to the mid-follicular phase-was recently established by immunohistochemistry using a monoclonal antibody to the estrogen receptor [12], although the binding specificity, Kd, and NBSma are obviously unknown. The K d values (indicating the degree of the binding affinity) of the follicles obtained in the present study were not much different from those of the estrogen receptors already reported in the ovary [10,18,28], uterus [10,31], hypothalamus [32][33][34][35], and pituitary [32][33][34][35] of mammals, or in target estrogen organ tissues of birds [20,36,37].…”

Section: Discussioncontrasting

confidence: 74%

“…Cytosolic (soluble fraction in hypotonic buffer solution) and nuclear (insoluble fraction in hypotonic buffer solution) fractions of the large antral follicles or the ovarian remnants of rats and hamsters were prepared by homogenizing in hypotonic TEDB buffer (10 mM Tris-HCI, 1.5 mM EDTA, 1 mM dithiothreitol, pH 7.4) at 4°C according to the method of Kawashima et al [20,21]. The tissues were homogenized in hypotonic TEDB buffer with a Potter-Elvehjem-type glass-teflon homogenizer.…”

Section: Preparation Of Cytosolic and Nuclear Fractionsmentioning

confidence: 99%

“…The specific binding of [ 3 H]E 2 was measured by an exchange procedure using the DCC technique [22], with the modifications reported earlier for estrogen [20] and androgen [21] receptors. The diluent used was TEDB buffer.…”

Section: Determination Of Estrogen Bindingsmentioning

confidence: 99%

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Comparison of Follicular Estrogen Receptors in Rat, Hamster, and Pig1

Kawashima

Greenwald

1993

Biology of Reproduction

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The hypophysectomized or intact hamster--unlike the rat--does not respond to estrogen by increasing the number of large antral follicles. In the pig, estrogen inhibits progesterone synthesis by granulosa cells from large follicles for 36 h before progesterone accumulation is enhanced. The objective of this study was to determine whether these divergent responses of the three species to estrogen are related to differences in follicular estrogen receptors. For the rat antral follicle, the equilibrium dissociation constants (Kds), expressed as nmol/L, were 0.55 +/- 0.07 in the cytosolic fraction and 0.46 +/- 0.11 in the nuclear fraction; for the hamster and pig follicles, the respective Kds were 1.08 +/- 0.04 and 0.94 +/- 0.11, and 1.16 +/- 0.23 and 1.09 +/- 0.17, respectively. The cytosolic and nuclear Kds for the rat follicle were statistically different from those of the other two species, but this is most likely not of biological consequence. Comparison of the maximal numbers of binding sites (NBSmax) in the cytosolic fractions of the antral follicles, expressed as fmol/mg protein, indicated a relationship of hamster > rat = pig; whereas in the nuclear fraction, with the NBSmax expressed as fmol/mg DNA, the relationship was hamster > pig > rat. The total number of estrogen receptors (sum of the NBSmax of cytosolic and nuclear fractions) was approximately tenfold higher in the hamster than in the rat. Thus, the failure of hamster large follicles to respond in vivo or in vitro to estrogen cannot be explained on the basis of lack of estrogen receptors.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussioncontrasting

confidence: 74%

Section: Preparation Of Cytosolic and Nuclear Fractionsmentioning

confidence: 99%

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Comparison of Follicular Estrogen Receptors in Rat, Hamster, and Pig1

Kawashima

Greenwald

1993

Biology of Reproduction

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“…Previous studies using radio-labeled estradiol uptake demonstrated the presence of ER in the hen pituitary gland (Kawashima et al 1987(Kawashima et al , 1993. The cell types containing ER in mammals include gonadotropes (Friend et al 1994;Zafar et al 1995;Sheng et al 1998;Nishihara et al 2000;Tobin et al 2001), thyrotropes (Friend et al 1994;Stefaneanu et al 1994;), lactotropes (Friend et al 1994;Stefaneanu et al 1994;Zafar et al 1995;Nishihara et al 2000), somatotropes (Friend et al 1994;Stefaneanu et al 1994;Zafar et al 1995;Scanlan and Skinner 2002), corticotropes (Friend et al 1994;Stefaneanu et al 1994) and FS cells (Mitchner et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Cell-specific distributions of estrogen receptor alpha (ERα) and androgen receptor (AR) in anterior pituitary glands from adult cockerels as revealed by immunohistochemistry

et al. 2012

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Estrogens and androgens play important roles in regulating the hormone-secreting functions of the pituitary gland by binding to their corresponding receptors. However, the expression of estrogen receptors (ERs) and the androgen receptor (AR) and the cell types containing ERs and AR in the anterior pituitary gland of adult chickens have not been well-studied. In this study, the distribution of ERα, AR and their corresponding cell types in the anterior pituitary gland of adult cockerels was detected by immunohistochemistry. The results showed that ERα was expressed in 68.63 % of luteinizing hormone (LH) producing cells but was not found in thyrotropes, lactotropes, somatotropes, corticotropes and folliculo-stellate (FS) cells. Pituitary hormone and AR double labeling results showed that about 37 % of LH cells and 50 % of thyroid-stimulating hormone (TSH) producing cells expressed AR, respectively. In contrast, less than 1 % of the somatotropes had an AR positive signal and AR signals were not detected in lactotropes, corticotropes or FS cells. In addition, there were only a few AR and ERα dual-labeled cells observed. These novel results provide evidence for a cell-specific distribution of ERα and AR in the anterior pituitary from adult cockerels by immunohistochemistry. The different distributions of ERα and AR in the LH cells suggest that the feedback-regulating mechanisms of estrogen and androgen on the pituitary hormones secretion are different. The functions and related mechanisms still need to be elucidated further.

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“…A direct action of progesterone on the pituitary of the hen has been demonstrated by the presence of its receptors in cytosolic (KAWASHIMA et al, 1978) and nuclear (KAWASHIMA et al, 1979) fractions of the hen pituitary tissue and by the stimulation of production of LH in isolated pituitary cells in vitro (KAWASHIMA et al, 1982). Besides the progesterone receptor, estrogen KAWASHIMA et al, 1987) and androgen (KAWASHIMA et al, 1989) receptors are also present in the hen pituitary.…”

Section: Introductionmentioning

confidence: 99%