Presence of exoY, exoS, exoU and exoT genes, antibiotic resistance and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran

Azimi, Somayeh; Kafil, Hossein Samadi; Baghi, Hossein Bannazadeh; Shokrian, Saeed; Najaf, Khadijeh; Asgharzadeh, Mohammad; Yousefi, Mehdi; Shahrivar, Firooz; Aghazadeh, Mohammad

doi:10.3205/dgkh000264

Cited by 18 publications

(10 citation statements)

References 35 publications

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“…While in our work the prevalence of exoT was 5.7%, which was similar to a study in northwestern Iran (5%) [5], it was markedly lower than those reported by others. For example, in central Iran [12] and in Poland [23], the prevalence rates of 20.4 and 94.4% were reported, respectively.…”

Section: Discussionsupporting

confidence: 84%

“…For example, in central Iran [12] and in Poland [23], the prevalence rates of 20.4 and 94.4% were reported, respectively. Moreover, we observed a low prevalence of the exoY (12.8%); while, in the northwest of Iran, 55% [5] and in India, 91.3% [26] of P. aeruginosa isolates carried the exoY. The prevalence of the fourth gene, exoU in our study was 8.6%.…”

Section: Discussioncontrasting

confidence: 54%

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Relationship between type III secretion toxins, biofilm formation, and antibiotic resistance in clinical <i>Pseudomonas aeruginosa</i> isolates

Derakhshan

Rezaee

Mohammadi

2021

Russian Journal of Infection and Immunity

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Background and aim. Pseudomonas aeruginosa is considered as a notorious pathogen due to its multidrug resistance and life threatening infections. We investigated the relationship between type III secretion toxins, biofilm formation, and antibiotic resistance among clinical P. aeruginosa isolates. Methods. A total of 70 genetically distinct clinical P. aeruginosa isolates were characterized for antibiotic resistance by disk diffusion assay. Biofilm formation was evaluated by microtiter plate method and presence of four exo genes (exoS, exoU, exoT and exoY) was investigated by PCR. A p-value < 0.05 was regarded statistically significant. Results. The most effective antibiotics were Meropenem and Piperacillin. Multidrug resistance was more prevalent in the ciprofloxacin-resistant isolates than in the susceptible isolates. The most frequently identified exo was exoS (37.1%). Genotype exoS/exoT was found in 4 isolates, while genotype exoU/exoT was not found. Prevalence of exoS was generally higher in the susceptible isolates than in the resistant isolates. A significant association was found between the formation of strong biofilm and resistance to antibiotics (p < 0.05). Prevalence of exoY and exoU was higher in the non-strong biofilm producers compared to the strong biofilm producers. Conclusion. Our study revealed formation of strong biofilm along with antibiotic resistance and the presence of exo genes in P. aeruginosa isolates. Knowledge of virulence gene profiles and biofilm formation may be useful in deciding appropriate treatment.

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Section: Discussionsupporting

confidence: 84%

Section: Discussioncontrasting

confidence: 54%

Relationship between type III secretion toxins, biofilm formation, and antibiotic resistance in clinical <i>Pseudomonas aeruginosa</i> isolates

Derakhshan

Rezaee

Mohammadi

2021

Russian Journal of Infection and Immunity

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“…ExoS, ExoT, ExoU, and ExoY, four effector proteins of the T3SS, have been identified in P. aeruginosa . 42 , 43 In this study, the prevalence of the exoT gene in all CRPA isolates was 100%, followed by exoY (98.3%), exoU (94.9%), and exoS (6.8%). Although P. aeruginosa carries genes encoding T3SS, most strains do not have all four genes as a complete set.…”

Section: Discussionmentioning

confidence: 51%

Epidemiology, Molecular Characteristics, and Virulence Factors of Carbapenem-Resistant Pseudomonas aeruginosa Isolated from Patients with Urinary Tract Infections

Park¹,

SH²

2022

IDR

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Purpose Pseudomonas aeruginosa is an important pathogen that causes urinary tract infections. Carbapenems are the drugs of choice for the treatment of P. aeruginosa infections. However, the emergence and spread of carbapenem-resistant P. aeruginosa (CRPA) is a serious global health threat. In this study, we investigated the epidemiology, molecular characteristics, drug resistance, and virulence factors of CRPA isolated from urine samples. Methods A total of 124 P. aeruginosa isolates were obtained from urine samples collected between March 2020 and February 2021. Clonal relatedness was evaluated using multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). We performed antimicrobial susceptibility tests and investigated the presence of carbapenemase genes and virulence factors in CRPA isolates. Results The carbapenem resistance rate of the isolated P. aeruginosa was 46.7% (59/124). A total of 54 (91.5%) out of the 59 CRPA isolates were identified as multidrug-resistant. The majority of the CRPA isolates (81.4%, 48/59) harbored carbapenemase genes, such as bla IMP-6 or bla NDM-1 . In an epidemiological analysis using MLST, 88.1% of CRPA isolates were confirmed to be ST773 (50.8%, 30/59) or ST235 (37.3%, 22/59). The CRPA isolates harboring bla IMP-6 and bla NDM-1 belonged to ST235 (PFGE pulsotypes E1-E18, F) and ST773 (PFGE pulsotypes H1-H2, I1-I16) subtypes, respectively. The studied CRPA isolates simultaneously harbored 10 to 14 virulence factors of the 16 virulence factors examined. Nine virulence factor genes ( toxA, exoT, plcH, plcN, phzM, phzS, lasB, aprA , and algD ) were identified in all CRPA isolates. Conclusion Our study shows that P. aeruginosa ST235 harboring bla IMP-6 and ST773 harboring bla NDM-1 —known internationally as high-risk clones with multiple virulence factors—are widely spread in the study area. These findings suggest that continuous monitoring is necessary to prevent the further spread of carbapenemase-producing CRPA.

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“…The resulting clade system will hopefully simplify understanding of the bacterium and paths to treatment. (Filloux, 2011;Azimi et al, 2016).…”

Section: Secretory Factorsmentioning

confidence: 99%

Pseudomonas aeruginosa reference strains PAO1 and PA14: A genomic, phenotypic, and therapeutic review

Grace

Sahu²,

Owen³

et al. 2022

Front. Microbiol.

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Pseudomonas aeruginosa is a ubiquitous, motile, gram-negative bacterium that has been recently identified as a multi-drug resistant pathogen in critical need of novel therapeutics. Of the approximately 5,000 strains, PAO1 and PA14 are common laboratory reference strains, modeling moderately and hyper-virulent phenotypes, respectively. PAO1 and PA14 have been instrumental in facilitating the discovery of novel drug targets, testing novel therapeutics, and supplying critical genomic information on the bacterium. While the two strains have contributed to a wide breadth of knowledge on the natural behaviors and therapeutic susceptibilities of P. aeruginosa, they have demonstrated significant deviations from observations in human infections. Many of these deviations are related to experimental inconsistencies in laboratory strain environment that complicate and, at times, terminate translation from laboratory results to clinical applications. This review aims to provide a comparative analysis of the two strains and potential methods to improve their clinical relevance.

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Presence of exoY, exoS, exoU and exoT genes, antibiotic resistance and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran

Cited by 18 publications

References 35 publications

Relationship between type III secretion toxins, biofilm formation, and antibiotic resistance in clinical <i>Pseudomonas aeruginosa</i> isolates

Relationship between type III secretion toxins, biofilm formation, and antibiotic resistance in clinical <i>Pseudomonas aeruginosa</i> isolates

Epidemiology, Molecular Characteristics, and Virulence Factors of Carbapenem-Resistant Pseudomonas aeruginosa Isolated from Patients with Urinary Tract Infections

Pseudomonas aeruginosa reference strains PAO1 and PA14: A genomic, phenotypic, and therapeutic review

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