Presence of fucosamine in teichuronic acid of the alkalophilic <i>Bacillus</i> strain C-125

Aono, Rikizo; Uramoto, Mari

doi:10.1042/bj2330291

Cited by 21 publications

(15 citation statements)

References 16 publications

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“…Amino sugars were assayed by the Elson-Morgan reaction (Wheat, 1966) using glucosamine or fucosamine as a reference standard. Authentic fucosamine was prepared from the walls of B. lentus C-125 (Aono & Uramoto, 1986). L-Glutamic acid content was determined by using Lglutamic acid dehydrogenase and diaphorase (Beutler & Michal, 1974).…”

Section: Methodsmentioning

confidence: 99%

“…The cell-wall preparations contained the following amounts of uronic acids (pmol 1-1 OD,,,-'): C-125, 53; C-125-11, 23; C-125-Fl9, 67; C-125-90, 55. The insoluble material remaining after repeated TCA extraction represents only 2-4 % of the original uronic acids and consists mainly of peptidoglycans (Aono et al, 1984). The TCA extracts contained little peptidoglycan.…”

Section: Extraction Of the Non-peptidoglycan Components From The Cellmentioning

confidence: 99%

“…The amount of peptidoglycan was calculated by multiplying the measured DAP content by 884 (pg pmol-l). This factor has been experimentally determined on the basis of the chemical composition of peptidoglycan prepared from strain C-125 (Aono et al, 1984). In the cell walls, fucosamine is Nacetylated (Aono, 1985).…”

Section: Culture-ph-dependent Accumulation Of the Acidic Constituentsmentioning

confidence: 99%

“…teichuronic acid (TUA) and teichuronopeptide (TUP). The peptidoglycan is an Aly type that is identical to that in the cell walls of the neutrophilic Bacilltrs strbtilis (Aono et al, 1984). The TUA consists of galacturonic acid, glucuronic acid and N-acetyl-~-2-amino-2,6-dideoxygalactose (fucosamine) in a molar ratio of 1 : 1 : 1 (Aono & Uramoto, 1986).…”

Section: Introductionmentioning

confidence: 99%

“…The peptidoglycan is an Aly type that is identical to that in the cell walls of the neutrophilic Bacilltrs strbtilis (Aono et al, 1984). The TUA consists of galacturonic acid, glucuronic acid and N-acetyl-~-2-amino-2,6-dideoxygalactose (fucosamine) in a molar ratio of 1 : 1 : 1 (Aono & Uramoto, 1986). The TUP is a polymer in which polyglutamic acid binds covalently to polyglucuronic acid (PGU) and has a 1 : 4.5 molar ratio of glucuronic acid to Lglutamic acid (Aono, 1985(Aono, ,1987(Aono, ,1989.…”

Section: Introductionmentioning

confidence: 99%

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A high cell wall negative charge is necessary for the growth of the alkaliphile Bacillus lentus C-125 at elevated pH

et al. 1995

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The structural components in cell walls of three mutants of a facultative alkaliphile, Bacillus lentus C-125, defective in certain cell-wall components were characterized in detail. The cell walls of the wild-type C-125 were thick and increased in thickness when grown at high pH. Electron microscopy showed that triple layers developed when the bacteria were grown in an alkaline environment. In contrast, cell walls of teichuronopeptide (1UP)-defective mutants consisted of a single layer. For both the wild-type and mutants, the cell-wall concentrations o f the acidic structural polymers teichuronic acid and TUP increased with respect to peptidoglycan as culture pH increased. For all four strains, the anion content of their cell walls was the greatest at high pH. The cell-wall density of the negatively charged compounds (uronic acids plus L-glutamic acid) was calculated as about 3 and 9 equivalents (I cell wall region)-' for C-125 cells grown at pH 7 and 10, respectively. A t high pH, the specific growth rates of the two TUPdefective mutants were much lower than those of the wild-type. It is concluded that increased levels of acidic polymers in the cell walls of alkaliphilic bacteria may be a necessary adaptation for growth at elevated pH.

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Section: Methodsmentioning

confidence: 99%

Section: Extraction Of the Non-peptidoglycan Components From The Cellmentioning

confidence: 99%

Section: Culture-ph-dependent Accumulation Of the Acidic Constituentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%