Presence of Herpes-type Virus in the Culture Cell Line from a Nasopharyngeal Carcinoma in Taiwan

Sugano, Haruo; Takada, M; Chen, Hai-Chin; Tu, Shin-Mien

doi:10.2183/pjab1945.46.453

Cited by 8 publications

(1 citation statement)

References 4 publications

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“…Both fibroblast types were maintained in Dulbecco’s modified Eagle’s medium (DMEM) and Ham’s nutrient mixture F12 culture medium, supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin. The FaDu (ATCC HTB-43) and TW204 (from a Chinese nasopharyngeal carcinoma in Taiwan) cell lines [ 32 ], originated from the human hypopharynx geal and nasopharyngeal squamous cell carcinomas, were cultured in a RPMI-1640 medium supplemented with 10% FBS, 2 mM glutamine, 1% penicillin/streptomycin, 1% sodium pyruvate, and 1% of amino-acid solution. Cells were treated for 6 h with recombinant IL33 protein with doses of 50 and 25 μg/mL AMD3100 (Sigma, Zwijndrecht, The Netherlands) [ 33 ] and then, in a series of experiments, were pre-treated for 1 h with various enzymatic inhibitors of different signaling pathways: 50 μM FR180204 (ERK, inhibitor), 10 μM SB203580 (p38MAPK inhibitor) or 4 μM SP600125 (JNK inhibitor).…”

Section: Methodsmentioning

confidence: 99%

Interleukin-33-Enhanced CXCR4 Signaling Circuit Mediated by Carcinoma-Associated Fibroblasts Promotes Invasiveness of Head and Neck Cancer

Lin

Huang

Lee

et al. 2021

Cancers

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Despite recent advances, treatment for head and neck squamous cell carcinoma (HNSCC) has limited efficacy in preventing tumor progression. We confirmed previously that carcinoma-associated fibroblasts (CAF)-induced interleukin-33 (IL-33) contributed to cancer progression. However, the molecular mechanisms underlying the complex communication network of the tumor microenvironment merited further evaluation. To simulate the IL-33-induced autocrine signaling, stable clones of IL-33-overexpressing HNSCC cells were established. Besides well-established IL-33/ST2 and SDF1/CXCR4 (stromal-derived factor 1/C-X-C motif chemokine receptor 4) signaling, the CAF-induced IL-33 upregulated CXCR4 via cancer cell induction of IL-33 self-production. The IL-33-enhanced-CXCR4 regulatory circuit involves SDF1/CXCR4 signaling activation and modulates tumor behavior. An in vivo study confirmed the functional role of IL-33/CXCR4 in tumor initiation and metastasis. The CXCR4 and/or IL-33 blockade reduced HNSCC cell aggressiveness, with attenuated invasions and metastases. Immunohistochemistry confirmed that IL-33 and CXCR4 expression correlated significantly with disease-free survival and IL-33-CXCR4 co-expression predicted a poor outcome. Besides paracrine signaling, the CAF-induced IL-33 reciprocally enhanced the autocrine cancer-cell self-production of IL-33 and the corresponding CXCR4 upregulation, leading to the activation of SDF1/CXCR4 signaling subsequent to cancer progression. Thus, targeting the IL-33-enhanced-CXCR4 regulatory circuit attenuates tumor aggressiveness and provides a potential therapeutic option for improving the prognosis in HNSCC patients.

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Section: Methodsmentioning

confidence: 99%

Interleukin-33-Enhanced CXCR4 Signaling Circuit Mediated by Carcinoma-Associated Fibroblasts Promotes Invasiveness of Head and Neck Cancer

Lin

Huang

Lee

et al. 2021

Cancers

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Antibodies to herpes-type virus in nasopharyngeal carcinoma and control groups

Lin

Yang

et al. 1972

Cancer

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Sera collected from patients with nasopharyngeal carcinoma (NPC), in Taiwan, were titrated for antibodies to herpes‐type virus (HTV) in a Burkitt's lymphoma cell line (P3HR‐1) by the indirect immunofluorescence antibody technique. The results were compared with those of neighborhood controls matched by age and sex, with patient families and with neighborhood control families. Antibody liters were higher in the NPC cases than in any of the 3 control groups. Dissociations in the frequency distributions of antibody titers in the NPC and healthy control groups were found to be maximum when the limiting value was set at 1:640. There were no significant differences in the frequency distribution among the 3 control groups. Of 117 NPC patients, 40% had anti‐HTV titers equal to or greater than 1:640, while such values occurred in only 5, 5, and 6%, respectively, of the neighborhood controls, patient families, and control families. Though not statistically significant, there was difference in the percentages of “sero‐positive” cases for the NPC patients by sex—42% for males and 35% for females. There was no sex difference, or it was a little higher in females, in the 3 control groups. The geometric mean titers were 1:242, 1:65, 1:69, and 1:61, respectively, for the NPC patients, neighborhood controls, patient families, and neighborhood control families. There was a difference in the geometric means by sex in the patients, i.e., 1:253 and 1:210, respectively, for males and females, while no differences by sex were noted in the 3 control groups. Ridit analysis and chi square test showed definitely different results between the NPC patients and the 3 control groups. The relative risks indicated that persons with antibody titer of 1:2560 had more than 200 times the NPC risk of those with antibody titer of less than 1:40. There were no indications of household aggregation of high anti‐HTV titer (≥ 1:640) in the patients and control families. The significance and implications of these findings are discussed.

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Potential Viral Etiology of Human Tumors

Sugano¹

1975

Geschwülste · Tumors II

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Presence of Herpes-type Virus in the Culture Cell Line from a Nasopharyngeal Carcinoma in Taiwan

Abstract: No. 5]Proc. Japan Acad., 46 (1970) 453 107.

Cited by 8 publications

References 4 publications

Interleukin-33-Enhanced CXCR4 Signaling Circuit Mediated by Carcinoma-Associated Fibroblasts Promotes Invasiveness of Head and Neck Cancer

Interleukin-33-Enhanced CXCR4 Signaling Circuit Mediated by Carcinoma-Associated Fibroblasts Promotes Invasiveness of Head and Neck Cancer

Antibodies to herpes-type virus in nasopharyngeal carcinoma and control groups

Potential Viral Etiology of Human Tumors

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