Presence of IL-8 Gene Polymorphism&amp;nbsp;and IL-8 Serum Levels in Patients with Chronic Periodontitis - Literature Review

Mlachkova, Antoaneta; Popova, Christina; Doseva, Velichka

doi:10.3897/folmed.62.e47722

Cited by 17 publications

(13 citation statements)

References 33 publications

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“…IL-6, a proinflammatory factor secreted by macrophages and lymphocytes, can promote the generation of neutrophils, induce a systemic immune response, and promote inflammation[ 18 ]. IL-8 is an inflammatory chemokine that potentially activates neutrophils, increases lysozyme release, induces phagocytic effects, and causes inflammatory tissue damage[ 19 ]. TNF-α, a proinflammatory factor synthesized by mononuclear macrophages, can promote the activation of proinflammatory factors and aggravate the inflammatory response[ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Sequential treatment of severe pneumonia with respiratory failure and its influence on respiratory mechanical parameters and hemodynamics

Niu¹,

Wang²,

Li³

et al. 2022

WJCC

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BACKGROUND The pathophysiological characteristics of severe pneumonia complicated by respiratory failure comprise pulmonary parenchymal changes leading to ventilation imbalance, alveolar capillary injury, pulmonary edema, refractory hypoxemia, and reduced lung compliance. Prolonged hypoxia can cause acid-base balance disorder, peripheral circulatory failure, blood-pressure reduction, arrhythmia, and other adverse consequences. AIM To investigate sequential mechanical ventilation’s effect on severe pneumonia complicated by respiratory failure. METHODS We selected 108 patients with severe pneumonia complicated by respiratory failure who underwent mechanical ventilation between January 2018 and September 2020 at the Luhe Hospital’s Intensive Care Unit and divided them into sequential and regular groups according to a randomized trial, with each group comprising 54 patients. The sequential group received invasive and non-invasive sequential mechanical ventilation, whereas the regular group received invasive mechanical ventilation. Blood-gas parameters, hemodynamic parameters, respiratory mechanical parameters, inflammatory factors, and treatment outcomes were compared between the two groups before and after mechanical-ventilation treatment. RESULTS The arterial oxygen partial pressure and stroke volume variation values of the sequential group at 24, 48, and 72 h of treatment were higher than those of the conventional group ( P < 0.05). The carbon dioxide partial pressure value of the sequential group at 72 h of treatment and the Raw value of the treatment group at 24 and 48 h were lower than those of the conventional group ( P < 0.05). The pH value of the sequential group at 24 and 72 h of treatment, the central venous pressure value of the treatment at 24 h, and the Cst value of the treatment at 24 and 48 h were higher than those of the conventional group ( P < 0.05). The tidal volume in the sequential group at 24 h of treatment was higher than that in the conventional group ( P < 0.05), the measured values of interleukin-6 and tumor necrosis factor-α in the sequential group at 72 h of treatment were lower than those in the conventional group ( P < 0.05), and the total time of mechanical ventilation in the sequential group was shorter than that in the conventional group, with a statistically significant difference ( P < 0.05). CONCLUSION Treating severe pneumonia complicated by respiratory failure with sequential mechanical ventilation is more effective in improving respiratory system compliance, reducing inflammatory response, maintaining hemodynamic stability, and improving patient blood-gas levels; however, from this study’s perspective, it cannot reduce patient mortality.

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Section: Discussionmentioning

confidence: 99%

Sequential treatment of severe pneumonia with respiratory failure and its influence on respiratory mechanical parameters and hemodynamics

Niu¹,

Wang²,

Li³

et al. 2022

WJCC

View full text Add to dashboard Cite

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“…In addition, mRNA expression of the chemokine IL-8 was increased. IL-8 is an important factor in the regulation of the inflammatory response [32], promotes the migration of neutrophils from the peripheral blood to areas of inflammation in the gingival sulcus [33]. From the results of qRT-PCR that ICAM-1 expression was increased, the effect of LA on junctional epithelial cells may induce the migration of leukocytes into tissues and accelerate inflammation.…”

Section: Discussionmentioning

confidence: 99%

The Role of Lactic Acid on Wound Healing, Cell Growth, Cell Cycle Kinetics, and Gene Expression of Cultured Junctional Epithelium Cells in the Pathophysiology of Periodontal Disease

et al. 2021

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Lactic acid (LA) is short-chain fatty acid, such as butyric acid and propionic acid, that is produced as a metabolite of lactic acid bacteria, including periodontopathic bacteria. These short-chain fatty acids have positive effects on human health but can also have negative effects, such as the promotion of periodontal disease (PD), which is caused by periodontal pathogens present in the gingival sulcus. PD is characterized by apical migration of junctional epithelium, deepening of pockets, and alveolar bone loss. Thus, the junctional epithelial cells that form the bottom of the gingival sulcus are extremely important in investigating the pathophysiology of PD. The aim of this study was to investigate the effect of LA on wound healing, cell growth, cell cycle kinetics, and gene expression of cultured junctional epithelium cells. The results showed that stimulation with 10 mM LA slowed wound healing of the junctional epithelial cell layer and arrested the cell cycle in the G0/G1 (early cell cycle) phase, thereby inhibiting cell growth. However, cell destruction was not observed. LA also enhanced mRNA expression of integrin α5, interleukin (IL)-6, IL-8, intercellular adhesion molecule-1, and receptor activator of nuclear factor kappa-B ligand. The results of this study suggest that stimulation of junctional epithelial cells with high concentrations of LA could exacerbate PD, similarly to butyric acid and propionic acid.

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“…CRISPR–Cas9 was previously used to directly modify the Interleukin‐8 (IL‐8) gene 31 . IL‐8 gene polymorphisms have been shown to represent susceptibility factors in periodontal disease 32 . Through the generation of sgRNA vectors through CRISPR–Cas9, the researchers created HEK293 cells with specific IL‐8 haplotypes and tested their functionality in terms of primary neutrophil transmigration.…”

Section: Alternative Crispr–cas Systems As Potential Therapeutic Tools To Modify Transcriptome Expression Without Altering Genome Sequencmentioning

confidence: 99%

The role of CRISPR‐Cas in advancing precision periodontics

Barbour

Glogauer

Grinfeld

et al. 2021

J of Periodontal Research

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The significant advancement of molecular biology has revolutionized medicine and provided important technologies to further clinical research development. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are DNA sequences derived from bacteriophages which have previously infected the bacterial species. The CRISPR–Cas system plays a key role in bacterial defense by detecting and destroying DNA fragments during subsequent bacteriophage invasions. The Cas9 enzyme recognizes and cleaves new invading CRISPR‐complementary DNA sequences. Researchers have taken advantage of this biological device to manipulate microbes’ genes and develop novel therapeutics to tackle systemic disease. In this review, we discuss the potential of utilizing CRISPR–Cas systems in the periodontal field to develop personalized periodontal care. We summarize promising attempts to bring this technology to the clinical setting. Finally, we provide insights regarding future developments to best utilize the CRISPR–Cas systems to advance precision periodontics. Although further research is imperative to evaluate the safety and potential of using CRISPR–Cas to develop precision periodontics approaches, few studies showed promising data to support the investment into this important technology in the dental sector. CRISPR–Cas9 can be a useful tool to create knockouts in vitro and in vivo as a screening tool to identify cellular pathways involved in the pathogenesis of periodontitis. Alternative CRISPR systems such as CRISPRa, CRISPRi, and Cas13 can be used to modify the transcriptome and gene expression of genes involved in periodontitis progression. CRISPR systems such as Cas3 can be used to target the periodontal biofilm and to develop new strategies to reduce or eliminate periodontal pathogens. Currently, the utility of CRISPR–Cas applications in clinical settings is limited. Through this review, we hope to foster further discussion in the periodontal research and clinical communities with respect to the potential clinical application of novel, CRISPR–Cas based, therapeutics for periodontitis.

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Presence of IL-8 Gene Polymorphism and IL-8 Serum Levels in Patients with Chronic Periodontitis - Literature Review

Cited by 17 publications

References 33 publications

Sequential treatment of severe pneumonia with respiratory failure and its influence on respiratory mechanical parameters and hemodynamics

Sequential treatment of severe pneumonia with respiratory failure and its influence on respiratory mechanical parameters and hemodynamics

The Role of Lactic Acid on Wound Healing, Cell Growth, Cell Cycle Kinetics, and Gene Expression of Cultured Junctional Epithelium Cells in the Pathophysiology of Periodontal Disease

The role of CRISPR‐Cas in advancing precision periodontics

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