Presence of monoterpene synthase in four Labiatae species and Solid-Phase Microextraction- Gas chromatography-Mass Spectroscopy analysis of their aroma profiles

Abstract

Background:The family Lamiaceae (Labiatae) has included some medicinal plants. some monoterpene synthases, including linalool and limonene synthases, have been cloned and functionally characterized from several plants of Labiatae family.Materials and Methods:In this study, presence of linalool and limonene synthases, in four species of Labiatae family including Nepeta cataria, Lavandula angustifolia, Hyssopus officinalis and Salvia sclarea has been determined by molecular biological techniques together with the Head space SPME – GC-MS analysis of the aroma profile of these species.Results:Indicated that none of the plant species produced distinguishable bands with primer pairs related to d-limonene synthase. Distinguishable bands around 1800 bp in cDNA samples of L. angustifolia, H. officinalis and S. sclarea were observed regarding to the presence of linalool synthase. Head space SPME-GC-MS analysis of the aroma profiles of the above-mentioned plants showed that linalool (31.0%), linalyl acetate (18.2%), were found as the major compounds of L. angustifolia, while geraniol (5.5%), nerol (34.0%) and α- citral (52.0%) were identified as the main compounds of the N. cataria. The major components of H. officinalis and S. sclarea oils were determined as cis-pinocamphone (57.3%), and linalool (19.0%), linalyl acetate (51.5%), respectively.Conclusion:H. officinalis was rich of cyclic monoterpenes, L. angustifolia, N. cataria and S. sclarea showed considerable amount of linear monoterpenes. The aroma profile of the above-mentioned plants contained low concentration of sesquiterpenes except N. cataria, which indicated no sesquiterpene. The profiles of the main components of these plants are in agreement with molecular assays.

Agronomic Evaluation and Chemical Characterization of Sicilian Salvia sclarea L. Accessions

“…The essential oils of various species from the Lamiaceae family demonstrate a certain degree of chemical variability due to a range of factors [23][24][25][26][27][28]. Regarding the chemical composition of clary sage, various authors note that in most cases, the principal volatile compounds are terpenoids [29,30], among which linalyl acetate and linalool. These components are central to good quality oil for use as an aromatizing agent [6,12,14,31].…”

Cork ‐ a natural material for linalool controlled release

“…For the analysis of linalool, Headspace Solid Phase Microextration (HS‐SPME) coupled with gas chromatography (GC) with flame ionization detector (FID) was used. HS‐SPME is a simple, fast, and solvent‐free analytical technology and has been widely used in the analysis of volatile compounds . It is an efficient and sensitive method for extraction and pre‐concentration of target analytes, widely used in a broad range of applications due to organic solvent avoidance and interferences elimination .…”