Presence of Mycobacterium leprae DNA and PGL-1 antigen in household contacts of leprosy patients from a hyperendemic area in Brazil

Pinho, Jaqueline Diniz; Rivas, Priscila Marlys Sá; Mendes, Mariana Bonfim Pinto; Soares, Rms; Costa, Graciomar C.; Nascimento, Flávia R.F.; Paiva, Melville; Aquino, Dorlene Maria Cardoso de; Figueireido, I.A.; Santos, Alcione Miranda dos; Pereira, Silma Regina Ferreira

doi:10.4238/2015.november.18.10

Cited by 7 publications

(12 citation statements)

References 23 publications

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“…The high proportion of apparently healthy household contacts, and other members of the community with M. leprae nasal carriage, shows the efficacy of combining molecular and serological techniques to identify M. leprae carriers in the asymptomatic stage of infection, and highlights the importance of monitoring hyperendemic populations. 34,35 DNA detected in nasal swabs also indicates the presence of bacilli at the site of transmission and possible infection. The concomitant use of both molecular and serological assays enables the detection of subclinical infection in household contacts 35 and M. leprae carriers who could transmit and disseminate disease in endemic regions.…”

Section: Discussionmentioning

confidence: 99%

“…34,35 DNA detected in nasal swabs also indicates the presence of bacilli at the site of transmission and possible infection. The concomitant use of both molecular and serological assays enables the detection of subclinical infection in household contacts 35 and M. leprae carriers who could transmit and disseminate disease in endemic regions. 36 Chemoprophylaxis of these contacts is suggested.…”

Section: Discussionmentioning

confidence: 99%

“…36 Chemoprophylaxis of these contacts is suggested. 35 Another significant group included individuals with M. leprae in their blood (Table 1). It is not known whether these are healthy carriers or potential future patients.…”

Section: Discussionmentioning

confidence: 99%

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Leprosy Associated with Atypical Cutaneous Leishmaniasis in Nicaragua and Honduras

Soto

Caballero

Fuentes³

et al. 2017

The American Journal of Tropical Medicine and Hygiene

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In Central America, few cases of leprosy have been reported, but the disease may be unrecognized. Diagnosis is based on clinical criteria and histology. Preliminary field work in Nicaragua and Honduras found patients, including many children, with skin lesions clinically suggestive of atypical cutaneous leishmaniasis or indeterminate leprosy. Histology could not distinguish these diseases although acid-fast organisms were visible in a few biopsies. Lesions healed after standard antimicrobial therapy for leprosy. In the present study, patients, family members, and other community members were skin-tested and provided nasal swabs and blood samples. Biopsies were taken from a subgroup of patients with clinical signs of infection. Two laboratories analyzed samples, using local in-house techniques. , spp. and were detected using polymerase chain reactions. DNA was detected in blood samples and nasal swabs, including some cases where leprosy was not clinically suspected. spp. were also detected in blood and nasal swabs. Most biopsies contained DNA and coinfection of spp. with occurred in 33% of cases. DNA was also detected and sequenced from Nicaraguan and Honduran environmental samples. In conclusion, leprosy and leishmaniasis are present in both regions, and leprosy appears to be widespread. The nature of any relationship between these two pathogens and the epidemiology of these infections need to be elucidated.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Leprosy Associated with Atypical Cutaneous Leishmaniasis in Nicaragua and Honduras

Soto

Caballero

Fuentes³

et al. 2017

The American Journal of Tropical Medicine and Hygiene

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“…The ML-flow test is not a diagnostic method, but assists in the classification of patients and presents low cost and easy execution, making its use possible in health services routine, especially in regions where laboratory resources are not available [30,35,46,47]. Therefore, ML-flow is a methodology widely used in hyperendemic areas, especially in Brazil, where populations of Maranhão, São Paulo, Pará and Minas Gerais states were tested and reaffirm the importance of detection and control of disease cases through simple but reliable methodologies [32,[48][49][50][51][52].…”

Section: Lateral Flow Testsmentioning

confidence: 99%

“…In the same way, the methodology can be useful, for those patients with the pure neural form (PNL), who usually do not have cutaneous lesions and because of this they have deficient treatment scheme [65,66]. Further, the PCR technique may be useful in early identification, since a considerable number of studies have addressed the positivity of M. leprae DNA in contacts of leprosy patients [48,[67][68][69].…”

Section: Complementary Molecular Testsmentioning

confidence: 99%

Molecular and Biotechnological Approaches in the Diagnosis of Leprosy

Lima¹,

Moraes²,

Pinho³

et al. 2019

Hansen's Disease - The Forgotten and Neglected Disease

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Leprosy is a worldwide health problem, which needs the development of new and innovative strategies to be controlled. Early diagnosis of leprosy is an important contribution to reducing the incidence of the disease; thus, the development of biotechnology platforms, which include the mapping of antigens with potential to be used in immunodiagnostic and molecular methods for the detection of Mycobacterium leprae, is an important tool to confirm the clinical diagnostic. Molecular biology and biotechnological methods have been used to assist in the diagnosis of this disease, each one with its advantages and drawbacks. Enzyme-linked immunosorbent assay (ELISA) is the used method for leprosy diagnosis, and it allows the detection of infection-related antigens. Alternatively, due to their versatility to perform the same functions as the protein and non-protein natural antigens, mimetic peptides are considered an important tool. On the other hand, lateral flow assay (LFA) and optical and electrochemical biosensors are rapid and portable methods, capable of performing diagnosis in the field without sample preparation.This chapter presents such techniques, their uses in the diagnosis and detection of M. leprae, as well as the potential for the development of new techniques and strategies that can help to control and understand mycobacteriosis.

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Leprosy surveillance study in a highly endemic Brazilian area using leprosy specific serologic tests and IFNγ whole blood assay

Gonçalves

Hungria

Freitas

et al. 2020

Eur J Clin Microbiol Infect Dis

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Presence of Mycobacterium leprae DNA and PGL-1 antigen in household contacts of leprosy patients from a hyperendemic area in Brazil

Cited by 7 publications

References 23 publications

Leprosy Associated with Atypical Cutaneous Leishmaniasis in Nicaragua and Honduras

Leprosy Associated with Atypical Cutaneous Leishmaniasis in Nicaragua and Honduras

Molecular and Biotechnological Approaches in the Diagnosis of Leprosy

Leprosy surveillance study in a highly endemic Brazilian area using leprosy specific serologic tests and IFNγ whole blood assay

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