Presence of oxytalan fibers in human regenerated periodontal ligament

Abstract

The aim of the present study was to investigate whether oxytalan fibers are formed in the regenerated human periodontal ligament. 6 patients, each of them exhibiting an advanced intrabony defect, were treated with a bioresorbable membrane according to the GTR-principle. Following a healing period of 6 months, the teeth were extracted together with their surrounding soft and hard tissues and subsequently fixed in 10% buffered formalin. Following decalcification in EDTA, the specimens were embedded in paraffin and 8-microm histological sections were cut in the mesio-distal direction, parallel to the long axes of the teeth. The sections were stained with hematoxylin and eosin, or with the oxone-aldehyde-fuchsin-Halmi staining method and examined in the light microscope. A regenerated periodontal ligament containing newly-formed oxytalan fibers was observed in all specimens. Many of them inserted into the newly formed cementum on the root surface. It is concluded that oxytalan fibers are formed de novo in human regenerated periodontal ligament tissue.

Fibulin-5 Contributes to Microfibril Assembly in Human Periodontal Ligament Cells

“…For detection of fibrillin-1 and fibrillin-2, we partly separated matrix and cellular proteins from the cell/matrix samples as described previously [17]. …”

“…Depending on the relative proportions of microfibrils and elastin, elastic system fibers can be classified into three types: oxytalan, elaunin and elastic fibers. Interestingly, in the periodontal ligaments (PDL), only oxytalan fibers, which are pure microfibrils, are identified, whereas all three types of fibers are present in the gingiva [5, 17]. In PDL, oxytalan fibers are thought to have some functional roles, such as supporting of vascular orientation and regulation of vascular flow [18].…”

Gene Expression and Accumulation of Fibrillin-1, Fibrillin-2, and Tropoelastin in Cultured Periodontal Fibroblasts

“…Fibrillin-containing microfibrils are widely distributed throughout the body, either in association with elastin or in elastin-free bundles Zhang et al, 1995;Keene et al, 1997). The only components of the elastic system fibers that can be recognized in human periodontal ligaments are oxytalan fibers (Sculean et al, 1999), whereas all three types of fiber are found in human gingiva (Chavrier et al, 1988). Recently, we have reported that cultured human gingival fibroblasts (HGF) secrete both fibrillins and tropoelastin into the medium, whereas human periodontal ligament fibroblasts (HPLF) secrete only fibrillins (Tsuruga et al, 2002a).…”

Localization of Oxytalan Fiber, type III Collagen and BMP Family in Conventional and Desmoplastic Ameloblastoma

“…Among these matrix proteins, type III collagen and oxytalan connective tissue fibers purportedly formed the most significant components in the periodontal ligament and odontogenic ectomesenchyme [11][12][13][14][15][16] .…”