Presence of Papillomavirus DNA sequences in the canine transmissible venereal tumor (CTVT)

Abstract

BackgroundThe canine transmissible venereal tumor (CTVT) or Sticker’s sarcoma is a neoplastic disease affecting dogs. This disease is presented as a tumoral mass in the genital organs of both, male and female individuals. Up to date, there is no clear evidence indicating a viral agent as the causative mediator for CTVT development.PurposeThe present work aims to analyze 21 samples from canines with CTVT for molecular identification of Papillomavirus DNA sequences. In addition, microbiological analysis, cytologic and histopathologic evaluations were also performed.ResultsAll patients showed no biochemical and microbiological alterations. Molecular analysis demonstrated the viral DNA presence in the samples using different primer sets. The MY primers amplified a 450 bp band in seven out of 21 samples (33%). The PVF and Fap64 primer set, targeting the L1 sequence of Canine Papillomavirus (CPV), showed positivity in 16 out of 21 samples (76%).ConclusionThese results support the possible causative association between CPV and CTVT; nevertheless, additional studies are required to uphold such statement. This work presents evidence indicating that a viral agent might be involved in the pathogenesis of CTVT and set the bases for a better understanding of the CTVT pathobiology.

New insights into Canis familiaris papillomaviruses genetics and biology: Is the genetic characterization of CPV types and their variants an important clinical issue?

“…This tumor is present in the genital organ as a mass and is sexually transmissible. There is no definition of its etiology and further studies are necessary to determine whether CPV is involved in the development of this type of cancer and which type or variant might be responsible for the disease ( Ayala-Díaz et al, 2019 ).…”

Tumor venéreo transmissível em cavidade abdominal de cadela: Relato incomum

“…Os sinais clínicos variam de acordo com a localização do tumor, sendo que geralmente é observada a presença de uma ou mais massas nos órgãos genitais, secreção serosanguinolenta ou hemorrágica oriunda dos órgãos genitais e/ou das narinas e lambedura excessiva dessas regiões (Cohen, 1985;Rogers et al, 1998). Ao exame físico, o tecido tumoral apresenta-se com tamanhos variáveis, geralmente com coloração branco-acinzentada, rosada ou avermelhada; na maioria das vezes, são friáveis, hemorrágicos, com aspecto de couve-flor e, eventualmente, podem apresentar aparência necrótica (Ayala-Díaz et al, 2019;Das & Das, 2000;Oduye et al, 1973).…”

A First NGS Investigation Suggests No Association Between Viruses and Canine Cancers

“…Although individual tumor viruses exert their oncogenic effects in different ways, a common feature is represented by the integration of the viral genome into the host, causing gene mutations, chromosome instability, and genomic aberrations ( 7 , 8 ). In canine species, only few reports have pinpointed an association between viruses and tumors, and canine PV integration was described both in squamous cell carcinoma and transmissible venereal tumor ( 9 – 12 ). Although the presence alone of viral sequences in tumor genome is insufficient to prove an oncogenic role, viral integration may represent the first hallmark of the malignant transformation.…”