Presence of periodontopathic bacteria in coronary arteries from patients with chronic periodontitis

Marcelino, Silvia Linard; Gaetti‐Jardim, Elerson; Nakano, Viviane; Canônico, Luis A.D.; Nunes, Fábio Daumas; Lotufo, Roberto Fraga Moreira; Pustiglioni, Francisco Emílio; Romito, Giuseppe Alexandre; Avila-Campos, Mario Júlio

doi:10.1016/j.anaerobe.2010.08.007

Cited by 35 publications

(41 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Interestingly, in a separate study, P. gingivalis (most commonly) and other oral anaerobic organisms were identifi ed by PCR amplifi cation of 16S rRNA in 50 % of coronary atheromatous plaques in patients with periodontitis, where P. gingivalis is known to form subgingival biofi lms. This suggests the possibility that oral anaerobes may play a role in coronary artery disease (Marcelino et al 2010 ). Biofi lms were cultured in 23 explanted tracheal stents from children undergoing laryngotracheal reconstruction, 6 of which yielded anaerobic organisms of the genera Fusobacterium , Bacteroides , Actinomyces , Prevotella , Propionibacterium , Eubacterium and Veillonella (Simoni and Wiatrak 2004 ).…”

Section: Biofi Lm Studies Of Anaerobic Organismsmentioning

confidence: 99%

Biofilm Formation by Clinical Isolates and Its Relevance to Clinical Infections

Akers

Cardile

Wenke

et al. 2014

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

Reports of biofilms have increased exponentially in the scientific literature over the past two decades, yet the vast majority of these are basic science investigations with limited clinical relevance. Biofilm studies involving clinical isolates are most often surveys of isolate collections, but suffer from lack of standardization in methodologies for producing and assessing biofilms. In contrast, more informative clinical studies correlating biofilm formation to patient data have infrequently been reported. In this chapter, biofilm surveys of clinical isolates of aerobic and anaerobic bacteria, mycobacteria, and Candida are reviewed, as well as those pertaining to the unique situation of cystic fibrosis. In addition, the influence of host components on in vitro biofilm formation, as well as published studies documenting the clinical impact of biofilms in human infections, are presented.

show abstract

Section: Biofi Lm Studies Of Anaerobic Organismsmentioning

confidence: 99%

Biofilm Formation by Clinical Isolates and Its Relevance to Clinical Infections

Akers

Cardile

Wenke

et al. 2014

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

show abstract

“…The most likely role would be that of a nitric oxide detoxifier, as native NrfA has been reported to reduce nitric oxide as a substrate and to provide some tolerance to nitric oxide exposure in vivo [10,11]. This adaptation could help C. rectus to survive in its natural environment, which includes the oral cavity, where it contributes to periodontal disease, and within the circulatory system, where it has been reported to be present in arterial wall plaques [12,13]. Within the mouth, it is likely to come into contact with differing concentrations of free NO released to combat infection and from dietary nitrite, whereas, on systemic invasion, the bacterium is likely to be targeted by phagocytes.…”

Section: Active-site Conservation Among Cytochrome C Nitrite Reductasesmentioning

confidence: 95%

Characterization of the active site and calcium binding in cytochrome c nitrite reductases

Lockwood

Clarke

Butt

et al. 2011

Biochemical Society Transactions

View full text Add to dashboard Cite

The decahaem homodimeric cytochrome c nitrite reductase (NrfA) is expressed within the periplasm of a wide range of Gamma-, Delta- and Epsilon-proteobacteria and is responsible for the six-electron reduction of nitrite to ammonia. This allows nitrite to be used as a terminal electron acceptor, facilitating anaerobic respiration while allowing nitrogen to remain in a biologically available form. NrfA has also been reported to reduce nitric oxide (a reaction intermediate) and sulfite to ammonia and sulfide respectively, suggesting a potential secondary role as a detoxification enzyme. The protein sequences and crystal structures of NrfA from different bacteria and the closely related octahaem nitrite reductase from Thioalkalivibrio nitratireducens (TvNir) reveal that these enzymes are homologous. The NrfA proteins contain five covalently attached haem groups, four of which are bis-histidine-co-ordinated, with the proximal histidine being provided by the highly conserved CXXCH motif. These haems are responsible for intraprotein electron transfer. The remaining haem is the site for nitrite reduction, which is ligated by a novel lysine residue provided by a CXXCK haem-binding motif. The TvNir nitrite reductase has five haems that are structurally similar to those of NrfA and three extra bis-histidine-coordinated haems that precede the NrfA conserved region. The present review compares the protein sequences and structures of NrfA and TvNir and discusses the subtle differences related to active-site architecture and Ca2+ binding that may have an impact on substrate reduction.

show abstract

“…Bacterial DNA of oral origin has been frequently detected in atherosclerotic lesions suggesting that bacteria may affect atherogenesis and other systemic pathological conditions directly [43]. Transient bacteremias were shown in patients with PD after tooth brushing and following periodontal treatment [44e46].…”

Section: Two-way Relationship E Potential Mechanismsmentioning

confidence: 99%