Presence of Pheophytin and its Formation as a Chlorophyll Derivative in Selected Crop Species

Dissanayake, P.; Yamauchi, Naoki; Shigyo, Masayoshi

doi:10.4038/jas.v7i3.4876

Cited by 8 publications

(6 citation statements)

References 22 publications

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“…The chlorophyll degradation mechanisms might occur through the formation of chlorophyllide or pheophytins or both ways if the extract was not exposed to light, heat, and oxygen. In the degradation processes of leaves extract, the main reason for a change in structure from native chlorophyll to pheophytin is that core magnesium ion has lost been in the chlorophyll structure [22].…”

Section: Open Access Receivedmentioning

confidence: 99%

Performance of dye-sensitized solar cells extracted dye from wood apple leaves

et al. 2022

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In this work, wood apple leaves dye has been extracted, characterized, and examined as a potential photosensitizer for dye-sensitized solar cells (DSSCs). The dye was extracted in an ethanolic medium from the fresh wood apple leaves and characterized using thin-layer chromatography (TLC), ultraviolet-visible (UV-Vis), and Fourier transform infrared (FTIR) spectroscopy. The current density-voltage (J-V) characteristics measurements were performed on the two assembled DSSCs for 1- 22 days using fresh and seven days old extracted dye. The characterization results revealed that the extracted dye mainly contains the compound of carotenoids (neoxanthin), chlorophyll-a, chlorophyll-b, and their derivative (pheophytin) with various functional groups. The J-V characteristics of DSSCs indicate that an open-circuit voltage and short circuit current density radically decrease with increasing time, thus degrading the efficiency of cells. A degraded DSSCs suffered from high defect recombination may be induced by Mg ions migrating from chlorophyll dye into DSSC. Therefore, the extracted dye may be used for energy harvesting from the wood apple leaves.

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Section: Open Access Receivedmentioning

confidence: 99%

Performance of dye-sensitized solar cells extracted dye from wood apple leaves

et al. 2022

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“…However, in some plants Chlase was found not to be essential for dephytylation. Recent studies have shown that the chlorophyll degradation may progress through the formation of pheophytin a (Pheo a ) as a chlorophyll derivative (Schelbert et al, 2009 ; Dissanayake et al, 2012 ). Based on these findings, it is assumed that Chl degradation pathway is not necessarily via Chlide a but also involves Pheo a .…”

Section: Introductionmentioning

confidence: 99%

Melatonin Improves the Photosynthetic Apparatus in Pea Leaves Stressed by Paraquat via Chlorophyll Breakdown Regulation and Its Accelerated de novo Synthesis

2017

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The positive effect of melatonin on the function of the photosynthetic apparatus is known, but little is known about the specific mechanisms of melatonin's action in plants. The influence of melatonin on chlorophyll metabolism of 24-day-old Pisum sativum L. seedlings during paraquat (PQ)-induced oxidative stress was investigated in this study. Seeds were hydro-primed with water (H), 50 and 200 μM melatonin/water solutions (H-MEL50, H-MEL200), while non-primed seeds were used as controls (C). Increases in chlorophyllase activity (key enzyme in chlorophyll degradation) and 5-aminolevulinic acid contents (the first compound in the porphyrin synthesis pathway) were observed in H-MEL50 and H-MEL200 leaf disks. This suggests that melatonin may accelerate damaged chlorophyll breakdown and its de novo synthesis during the first hours of PQ treatment. Elevated level of pheophytin in control leaf disks following 24 h of PQ incubation probably was associated with an enhanced rate of chlorophyll degradation through formation of pheophytin as a chlorophyll derivative. This validates the hypothesis that chlorophyllide, considered for many years, as a first intermediate of chlorophyll breakdown is not. This is indicated by the almost unchanged chlorophyll to chlorophyllide ratio after 24 h of PQ treatment. However, prolonged effects of PQ-induced stress (48 h) revealed extensive discolouration of control and water-treated leaf disks, while melatonin treatment alleviated PQ-induced photobleaching. Also the ratio of chlorophyll to chlorophyllide and porphyrin contents were significantly higher in plants treated with melatonin, which may indicate that this indoleamine both retards chlorophyll breakdown and stimulates its de novo synthesis during extended stress. We concluded that melatonin added into the seeds enhances the ability of pea seedlings to accelerate chlorophyll breakdown and its de novo synthesis before stress appeared and for several hours after, while during prolonged PQ incubation melatonin delays chlorophyll degradation.

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“…We cannot exclude the possibility of its artifactual origin due to the extraction procedure. To the best of our knowledge, it has not been recognized before in species belonging to the Plantaginaceae family, being more common to find in algae and crops such as green tea leaves, parsley, bellpepper, and Japanese bunching onion . Compound 1 is known to exert several interesting pharmacological properties such as the antioxidant, antiviral, anti‐inflammatory, antichemotactic, antihepatitis C, and antitumor ones …”

Section: Resultsmentioning

confidence: 99%

“…To the best of our knowledge, it has not been recognized before in species belonging to the Plantaginaceae family, being more common to find in algae and crops such as green tea leaves, parsley, bellpepper, and Japanese bunching onion. [16] [17] Compound 1 is known to exert several interesting pharmacological properties such as the antioxidant, antiviral, anti-inflammatory, antichemotactic, antihepatitis C, and antitumor ones. [18 -21] Linariin (2) is a scutellarein derivative which represents a new phytochemical for the species while in the genus its presence has been reported before in K. elatine DUMORT as well as in botanically related entities of the Antirrhinae tribe such as in Linaria aucheri BOISS.…”

Section: Resultsmentioning

confidence: 99%

Iridoids of Chemotaxonomy Relevance, a New Antirrhinoside Ester and Other Constituents from Kickxia spuria subsp. integrifolia (Brot.) R.Fern.

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Serafini

et al. 2018

Chemistry & Biodiversity

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In this work, the secondary metabolite content of the EtOH extract of Kickxia spuria subsp. integrifolia (Brot.) R.Fern. is reported. Fourteen compounds were isolated and identified by means of column chromatography and NMR and MS instrumental techniques, respectively. Among the identified compounds, the chemotaxonomic markers of the species were evidenced, whereas others were reported for the first time in the genus. Among these, a new antirrhinoside derivative (12) was recognized. The iridoid content showed a molecular pattern very similar to those reported for other taxa comprised in the Antirrhinae tribe of Plantaginaceae, thus providing an additional evidence that supports the current botanical classification of the Kickxia genus. Anyway, most of the recognized components are able to exert important pharmacological properties which might suggest the possible employment of also this species in traditional medicine just like it happens for some other species of the Kickxia genus.

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Presence of Pheophytin and its Formation as a Chlorophyll Derivative in Selected Crop Species

Abstract: Most

Cited by 8 publications

References 22 publications

Performance of dye-sensitized solar cells extracted dye from wood apple leaves

Performance of dye-sensitized solar cells extracted dye from wood apple leaves

Melatonin Improves the Photosynthetic Apparatus in Pea Leaves Stressed by Paraquat via Chlorophyll Breakdown Regulation and Its Accelerated de novo Synthesis

Iridoids of Chemotaxonomy Relevance, a New Antirrhinoside Ester and Other Constituents from Kickxia spuria subsp. integrifolia (Brot.) R.Fern.

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