Presence of Plasmid-Mediated Quinolone Resistance in Klebsiella pneumoniae Isolates Possessing bla _KPC in the United States

Abstract

The presence of plasmid-mediated quinolone resistance genes [i.e., qnrA, qnrB, qnrS, aac(6)-Ib-cr, and qepA] was evaluated among 42 bla KPC -containing Klebsiella pneumoniae isolates collected in the eastern United States. One isolate carried the bla KPC-3 and qnrB19 genes on the same conjugative plasmid, whereas another carried the bla KPC-3 and qnrA1 genes on separate plasmids.

Emergence of KPC-2-Producing Salmonella enterica Serotype Schwarzengrund in Argentina

“…Using specific primers and sequencing (12), a qnrB19 gene was detected in the parental strain. These results were similar to those of previous reports which demonstrated that KPC-producing isolates are resistant not only to all ␤-lactam antibiotics, including carbapenems, but also to some non-␤-lactam antibiotics, such as fluoroquinolones (13,14).…”

Outbreak of Klebsiella pneumoniae carbapenemase 2-producing K. pneumoniae with high qnr prevalence in a Chinese hospital

“…P, K. pneumoniae K1 isolated from the 2nd Affiliated Hospital of Zhejiang University (producing KPC-2, TEM-1, SHV-11 and CTX-M-14); t, E. coli transconjugant; M, strain producing TEM-28 (pI 6.1), SHV-7 (pI 7.6) and ACT-1 (pI 9.0). Monteiro et al, 2009;Naas et al, 2008;Tsakris et al, 2008;Yigit et al, 2003) and some encoded qnr (Chmelnitsky et al, 2008;Endimiani et al, 2008;Mendes et al, 2008). Unlike these, most bla KPC -encoding plasmids in this study were associated with four drug-resistance genes.…”

The KPC type beta-lactamases: new enzymes that confer resistance to carbapenems in Gram-negative bacilli.

“…Soon afterward, KPC-3 was founded in Klebsiella pneumoniae [39] and in other Enterobacteriaceae isolated in USA [11,[40][41][42]. KPC-3 has never been reported outside the USA, until 2007.…”

“…The bla KPC genes have usually been identified in large plasmids. These plasmids also carry aminoglycosideresistance determinants, and have been associated with other antibiotic-resistance genes such as the most widespread ESBL genes or quinolones-resistance determinants (QnrA, QnrB) [25,31,40,[55][56][57].…”