Presence of the esp gene in Enterococcus faecium derived from oropharyngeal microbiota of haematology patients

Jovanović, M.; Tošić, Tanja; Jovanović, Svetlana P.; Stosović, R; Stevanović, Goran; Velebit, Branko; Zervos, Marcus J.

doi:10.1016/j.archoralbio.2018.01.008

Cited by 6 publications

(3 citation statements)

References 31 publications

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“…This is consistent with results from a 2018 Serbian study by Jovanović et al investigating VRE isolated from the oropharynx and stools of hemato-oncological patients. They also showed 100% presence of the vanA gene and 0% presence of the vanB gene [33].…”

Section: Discussionmentioning

confidence: 95%

Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients

et al. 2020

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Enterococci are important bacterial pathogens, and their significance is even greater in the case of vancomycin-resistant enterococci (VRE). The study analyzed the presence of VRE in the gastrointestinal tract (GIT) of hemato-oncological patients. Active screening using selective agars yielded VRE for phenotypic and genotypic analyses. Isolated strains were identified with MALDI-TOF MS, (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry) their susceptibility to antibiotics was tested, and resistance genes (vanA, vanB, vanC-1, vanC2-C3) and genes encoding virulence factors (asa1, gelE, cylA, esp, hyl) were detected. Pulsed-field gel electrophoresis was used to assess the relationship of the isolated strains. Over a period of three years, 103 VanA-type VRE were identified in 1405 hemato-oncological patients. The most frequently detected virulence factor was extracellular surface protein (84%), followed by hyaluronidase (40%). Unique restriction profiles were observed in 33% of strains; clonality was detected in 67% of isolates. The study found that 7% of hemato-oncological patients carried VRE in their GIT. In all cases, the species identified was Enterococcus faecium. No clone persisted for the entire 3-year study period. However, genetically different clusters were observed for shorter periods of time, no longer than eight months, with identical VRE spreading among patients.

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Section: Discussionmentioning

confidence: 95%

Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients

et al. 2020

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“…A decade ago, the presence of genes responsible for adhesion and biofilm formation has been rarely observed in the genome of E. faecium than E. faecalis [ 17 , 18 ]. However, recent publications indicate that the percentage of biofilm-forming E. faecium has been increasing [ 19 , 20 ]. Scientists have proved that in hospitals, antibiotics administered to patients, both in targeted and empirical therapy, often reach sub-inhibitory concentrations and enhance biofilm formation by enterococci [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance of Enterococcus spp. Isolated from the Urine of Patients Hospitalized in the University Hospital in North-Central Poland, 2016–2021

et al. 2022

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Urinary Tract Infections (UTIs) are common outpatient and inpatient infections, often treated with empirical therapy. Enterococcus spp. is responsible for about 10% of UTIs. This study aimed to determine the necessity of changing the empirical treatment of UTIs caused by Enterococcus spp. The evaluation was performed for 542 Enterococcus strains isolated from urine samples in the years 2016–2021. We identified three Enterococcus species that were found: E. faecalis (389, 71.8%), E. faecium (151, 27.8%) and E. gallinarum (2, 0.4%). E. faecalis was the dominant species every year. Among E. faecalis, the most prevalent was resistance to norfloxacin (51.4%). Almost all E. faecium strains (150, 99.3%) were resistant to beta-lactams and norfloxacin. Eighty-three strains (55.0%) were resistant to vancomycin and 72 (47.7%) to teicoplanin. E. faecium strains showed a significantly higher percentage of resistance mechanisms GRE (Glicopeptide-Resistant Enterococcus) (72, 48.7%) and VRE (Vancomycin-Resistant Enterococcus) (11, 7.3%), while only five strains of E. feacalis showed a VRE mechanism (1.3%). In the therapy of E. faecalis UTIs, ampicillin and imipenem still remain effective. However, the above-mentioned antibiotics, as well as fluoroquinolones, are not recommended in the treatment of UTIs of E. faecium etiology.

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“…Cytolysin increases enterococcal virulence and patient mortality ( Pillay, Zishiri & Adeleke, 2018 ). Esp is associated with enhanced virulence, attachment to an abiotic surface, colonization in the urinary tract and biofilm formation ( Jovanovic et al, 2018 ). While esp is encoded on a pathogenicity island in E. faecium and E. faecalis , hyl is specific to E. faecium ( Gok et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Epidemiological profiles and pathogenicity of Vancomycin-resistant Enterococcus faecium clinical isolates in Taiwan

Lin

Chan

Stern

et al. 2023

PeerJ

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The emerging Vancomycin-resistant Enterococcus faecium (VRE-fm) is an opportunistic pathogen causing nosocomial infections. The identification of VRE-fm is important for successful prevention and control in healthcare settings. VRE-fm clinical isolates obtained from regional hospitals in northern Taiwan were characterized for antimicrobial susceptibility, virulence genes and biofilm production. Most isolates exhibited multi-drug resistance and carried the virulence genes, esp and hyl. While all isolates produce biofilms, those isolates that carried esp exhibited greater biofilm production. Isolates with different virulence gene carriages were examined for pathogenicity by using a nematode model, Caenorhabditis elegans, for determining microbial-host interactions. The survival assay showed that C. elegans was susceptible to Linezolid-resistant VRE-fm isolates with hyl. Combining the molecular epidemiological profiles regarding pathogenesis in C. elegans can serve as a guide for physicians in limiting opportunistic infections caused by VRE-fm.

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Presence of the esp gene in Enterococcus faecium derived from oropharyngeal microbiota of haematology patients

Cited by 6 publications

References 31 publications

Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients

Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients

Antibiotic Resistance of Enterococcus spp. Isolated from the Urine of Patients Hospitalized in the University Hospital in North-Central Poland, 2016–2021

Epidemiological profiles and pathogenicity of Vancomycin-resistant Enterococcus faecium clinical isolates in Taiwan

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