Presence of the pharmaceutical drug carbamazepine in coastal systems: Effects on bivalves

Almeida, Ângela; Calisto, Vânia; Esteves, Valdemar I.; Schneider, R.; Soares, Amadeu M.V.M.; Figueira, Etelvina; Freitas, Rosa

doi:10.1016/j.aquatox.2014.08.002

Cited by 148 publications

(75 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The peroxidation of lipids by CBZ may also be species-specific and be influenced by the length of exposure. The study of Almeida et al (2014) supports these ideas. While in the clam Venerupis philippinrum exposed to 9 µg/l of CBZ, LPO increased, in Venerupis decussate the same dose of CBZ decreased LPO significantly.…”

Section: Discussionsupporting

confidence: 71%

Effects of the pharmaceutical contaminants ibuprofen, diclofenac, and carbamazepine alone, and in combination, on oxidative stress parameters in early life stages of tench (Tinca tinca)

Stancová¹,

Plhalová²,

Blahová³

et al. 2017

Vet. Med. - Czech

View full text Add to dashboard Cite

ABSTRACT:In the present study, the effects of sub-lethal sub-chronic doses of ibuprofen, diclofenac, and carbamazepine alone, and in combination (concentration range 0.02-60 µg/l), on the early life stages of tench (Tinca tinca) were investigated. The lower concentrations of pharmaceuticals tested (0.02, 0.2, 2 µg/l) represent the concentration values of these substances commonly present in surface waters or effluents from wastewater treatment plants. Multiple biomarkers of biotransformation, antioxidant defence systems, and lipid peroxidation were determined in fish after 35 days of exposure. The evaluated pharmaceuticals induced oxidative stress in fish both alone and in combination with each other. Generally, 60 µg/l of each single pharmaceutical influenced the activity of antioxidant enzymes significantly (P < 0.05), whereas the same concentration of these pharmaceuticals in combination (1 : 1 : 1) did not have any impact on the activity of these enzymes. However, changes in biotransformation and antioxidant enzymes were apparent if lower concentrations of these pharmaceuticals were administered in the mixture. Significant changes (P < 0.05) in the activities of glutathione reductase, glutathione peroxidase, and glutathione-S-transferase were observed even at environmental concentration ranges. A significant effect (P < 0.05) on lipid peroxidation levels was found only in the experimental group exposed to carbamazepine. Keywords: antioxidant defence system; lipid peroxidationList of abbreviations ANC = acid-neutralising capacity, CAT = catalase, CBZ = carbamazepine, COD Mn = chemical oxygen demand, COX = cyclooxygenase, DCF = diclofenac, DMSO = dimethylsulfoxide, GPx = glutathione peroxidase, GR = glutathione reductase, GSH = reduced active form of glutathione, GSSG = oxidised inactive form of glutathione, GST = glutathione-S-transferase, IBU = ibuprofen, LC = liquid chromatography, LC-MS/MS = liquid chromatography with tandem mass spectrometry, LPO = lipid peroxidation level, NSAIDs = non-steroidal anti-inflammatory drugs, ROS = reactive oxygen species Pharmaceutical mixtures that contaminate water sources are currently a problem worldwide. Ibuprofen (IBU), diclofenac (DCF), and carbamazepine (CBZ) are among the most frequently detected drugs in aquatic ecosystems. Their concentrations in surface water range from ng/l to tens of µg/l. Higher concentrations have been detected in developing countries due to the direct discharge of untreated wastewater from residences and hospitals into surface waters (Tran et al. 2014).

show abstract

Section: Discussionsupporting

confidence: 71%

Effects of the pharmaceutical contaminants ibuprofen, diclofenac, and carbamazepine alone, and in combination, on oxidative stress parameters in early life stages of tench (Tinca tinca)

Stancová¹,

Plhalová²,

Blahová³

et al. 2017

Vet. Med. - Czech

View full text Add to dashboard Cite

show abstract

“…A prolonged exposure to CBZ leads to a strong inhibition of antioxidant enzymes, compromising the destruction of ROS and the control of the oxidative stress, evidencing the higher toxicity that a chronic exposure to CBZ induces in comparison with an acute exposure, especially concerning GLYC and LPO levels and CYP3A4 activity (Almeida et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…philippinarum (Adams & Reeve, 1850), commonly known as the Manila clam, was selected to study the ecotoxicological impact of CBZ in the Ria de Aveiro. This species has already been used in previous studies, under laboratory and field conditions, to assess the toxicity of CBZ (Aguirre-Martínez et al, 2013a, 2013bAlmeida et al, 2014) as well as other pharmaceutical drugs (Contardo-Jara et al, 2011;Matozzo et al, 2012a;Antunes et al, 2013;Milan et al, 2013) and to assess organic and metal pollution (Riba et al, 2004;Martín-Díaz et al, 2007;Figueira et al, 2012). All these studies demonstrated the suitability of R. philippinarum as a bioindicator species in marine environment quality assessment.…”

Section: Study Area and Test Organismmentioning

confidence: 93%

“…Gagné et al (2006b) determined CBZ in the freshwater mussel (Elliptio complanata) soft tissue homogenate extracts using a competitive enzymatic immunoassay. In the present study, CBZ was quantified based on the direct competitive ELISA assay (Enzyme-Linked ImmunoSorbent Assay), according to the procedure developed by Bahlmann et al (2009) and the modifications referred by Almeida et al (2014). Good recoveries were previously demonstrated using ELISA to quantify CBZ in R. philippinarum homogenate extracts, with no matrix interferences and with no sample pre-treatment, turning the assay suitable for large and economic environmental screenings (Almeida et al, 2014).…”

Section: Immunoassay Procedures and Calibration Curvementioning

confidence: 96%

“…In the present study, CBZ was quantified based on the direct competitive ELISA assay (Enzyme-Linked ImmunoSorbent Assay), according to the procedure developed by Bahlmann et al (2009) and the modifications referred by Almeida et al (2014). Good recoveries were previously demonstrated using ELISA to quantify CBZ in R. philippinarum homogenate extracts, with no matrix interferences and with no sample pre-treatment, turning the assay suitable for large and economic environmental screenings (Almeida et al, 2014). This assay is based on a direct competition of the antigen (CBZ) and tracer (CBZ analogue linked to the enzyme horseradish peroxidase) for the primary antibody (monoclonal antibody against CBZ) binding sites that are immobilized in a 96-well microtiter plate via a secondary antibody.…”

Section: Immunoassay Procedures and Calibration Curvementioning

confidence: 99%

See 2 more Smart Citations

Chronic toxicity of the antiepileptic carbamazepine on the clam Ruditapes philippinarum

Almeida

Freitas

Calisto

et al. 2015

Comparative Biochemistry and Physiology Part C: Toxicology &

Self Cite

View full text Add to dashboard Cite

The impacts of carbamazepine (CBZ) on aquatic organisms are yet not well investigated. The present study aimed to better understand the chronic effects of environmentally relevant concentrations of CBZ. The experiment was performed by exposing the filter feeding clam Ruditapes philippinarum to 0.00, 0.03, 0.30, 3.00 and 9.00 μg/L, during 28 days. To assess the chronic toxicity of the drug a battery of biomarkers related with health status and oxidative stress was applied. In order to quantify CBZ in the clam's tissues and in water samples ELISA was used. The present study showed three types of responses on the clams after a chronic exposure to CBZ. For control condition and the lower concentrations (0.03 and 0.30 μg/L) a "similar" metabolic state was observed and the most efficient antioxidant status leading to the elimination of reactive oxygen species formed during the metabolism of CBZ. The concentration of 3.00 μg/L seemed to be a "threshold" concentration, beyond which the concentration levels of CBZ began to exert a toxic effect, compromising the activity of biotransformation and antioxidant enzymes, with notorious effects at the highest CBZ concentration (9.00 μg/L). CBZ also seemed to alter the energy-related responses, especially the glycogen and electron system responses, revealing a slowdown in metabolism at the higher exposure concentrations (3.00 and 9.00 μg/L). Overall, the present study demonstrated that the higher CBZ concentrations can lead to the impairment of antioxidant enzymes compromising the neutralization of reactive oxygen species, and thus the ability to cope with oxidative stress.

show abstract

Presence of Anthropogenic Markers in Water: A Case Study of the Guaporé River Watershed, Brazil

Bastos

Santos

Lima

et al. 2018

CLEAN Soil Air Water

View full text Add to dashboard Cite

Southern Brazil is an agricultural region that is experiencing strong growth in both cereal and animal production. The intensification of agricultural practices, as well as the growing urbanization in this region, also generates strong anthropogenic pressures on the aquatic environment. In Brazil, the lack of sewage treatment and the wide use of waste (liquid or solid) for the fertilization of soils are likely to favor the spread of pharmaceuticals in the surrounding environment. Considering the benefits of the determination of anthropogenic markers in the environment, the study aimed to monitor the presence of carbamazepine (CBZ) and sucralose (SCR) in the Guaporé River, representative of a southern Brazilian rural watershed. Both carbamazepine and sucralose are measured in water by polar organic chemical integrative samplers (POCIS) (CBZ: lower than limit of detection (< LOD) to 746 μg g−1 and SCR:

show abstract

Presence of the pharmaceutical drug carbamazepine in coastal systems: Effects on bivalves

Cited by 148 publications

References 73 publications

Effects of the pharmaceutical contaminants ibuprofen, diclofenac, and carbamazepine alone, and in combination, on oxidative stress parameters in early life stages of tench (Tinca tinca)

Effects of the pharmaceutical contaminants ibuprofen, diclofenac, and carbamazepine alone, and in combination, on oxidative stress parameters in early life stages of tench (Tinca tinca)

Chronic toxicity of the antiepileptic carbamazepine on the clam Ruditapes philippinarum

Presence of Anthropogenic Markers in Water: A Case Study of the Guaporé River Watershed, Brazil

Contact Info

Product

Resources

About