Presence of Vancomycin, Aminoglycosides, and Erythromycin Resistance Genes in Enterococci Isolated from Clinical Samples in Turkey

Çelik, Şule; Çakırlar, Fatma Köksal; Torun, Müzeyyen Mamal

doi:10.7754/clin.lab.2014.140211

Cited by 7 publications

(6 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…12 Several studies have showed that E. faecalis was the most common enterococcal isolate in clinical samples. [13][14][15] These findings are consistent with the results of this study, in which 91.25% of enterococcal isolates were E. faecalis. However, another report stated that E. faecium infections are of increasing frequency.…”

Section: Discussionsupporting

confidence: 91%

“…However, another report stated that E. faecium infections are of increasing frequency. 14 In the present study, 87.5% of enterococci exhibited multidrug resistance (MDR). In previous studies, the rate of MDR varied from 45% to 100%.…”

Section: Discussionmentioning

confidence: 61%

“…In contrast, Celik et al reported that antibiotic resistance percentages were higher in E. faecium than in E. faecalis. 14 The rate of HLGR in enterococci varies from 1% to 89% in different regions. 7,22,23 The most common gene associated with HLGR is aac(6´)-Ieaph(2)-Ia.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

High level aminoglycoside resistant enterococci inhospital-acquired urinary tract infections in Mansoura, Egypt

El-Mahdy

Mostafa

El-Kannishy

2018

Germs

View full text Add to dashboard Cite

Introduction Resistance to different antimicrobial agents is increasing in enterococci and effective treatment represents a major health concern. The aim of this study was to determine the antimicrobial resistance patterns and the frequency of high level aminoglycoside resistance (HLAR) among enterococci. Methods A total of 80 enterococcal isolates, (73 Enterococcus faecalis, 7 Enterococcus faecium) were collected from patients with hospital acquired urinary tract infections (UTI) at Mansoura University hospitals in Egypt. Antimicrobial susceptibility testing was performed via the disc diffusion method. PCR was used for identification of species and detection of aminoglycoside-modifying enzymes genes (AME). Results All enterococcal isolates were sensitive to vancomycin and linezolid. Fifty-three isolates exhibited HLAR. Our results show that HLAR was mediated by the presence of multiple AMEs genes. The aac(6´)-Ie-aph(2)-Ia gene was associated with aph(3´)-IIIa and ant(6)-Ia gene in 69% of HLAR isolates. Conclusion This study showed that enterococci isolated from hospital acquired UTI were resistant to multiple antibiotics. Furthermore, the frequency of high level gentamicin resistance (HLGR) was higher than high level of streptomycin resistance (HLSR). The most common AME genes were aph(3´)-IIIa and ant(6)-Ia followed by aac(6´)-Ie-aph(2)-Ia.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 61%

See 1 more Smart Citation

High level aminoglycoside resistant enterococci inhospital-acquired urinary tract infections in Mansoura, Egypt

El-Mahdy

Mostafa

El-Kannishy

2018

Germs

View full text Add to dashboard Cite

show abstract

“…Real-time PCR profile was used; denaturation step at 95°C for 10 min, followed by 40 cycles, of 10s at 95°C, [4]. In our country, the rates of E. faecium and E. faecalis were determined to be 15 -50% and 52 -85%, respectively [23,24]. In previous researches, Enterococcus spp., which can be found without losing their vitality for 4 months in inanimate environments [21].…”

Section: B) Molecular Detection Of Ses Pvl and Tsst -1 Genesmentioning

confidence: 87%

Should we leave the paper currency? A microbiological examination

Demırci

Celepler²,

Dinçer³

et al. 2020

Rev Esp Quimioter

View full text Add to dashboard Cite

“…This phenotype was determined by erm gene that product an erythromycin resistance methylase, inducing methylation of specific adenosine residues on the 23S rRNA, which causes resistance of newly synthesized ribosomes to macrolidelincosamide-streptogramin B antimicrobials [14,15]. Sule Celik et al described that the emergence of high-level erythromycin resistance may lead to poorer prognosis, more severe recurrence, and higher mortality, in enterococcal infections [26].…”

Section: Discussionmentioning

confidence: 99%

In vitro antibacterial activity of telithromycin against Enterococcus spp. isolated from China

Xiong

Chen

Sun

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Enterococci has resistant to a wide range of antimicrobials, and the treatment of enterococcal infection has always been an issue of concern. This study aimed to explore the new ketolides antimicrobials-telithromycin, against the planktonic cells and biofilms of enterococci. The minimum inhibitory concentration (MIC) of telithromycin was determined. The sequence types (STs) and genotypes of resistance to erythromycin in enterococci were detected. Furthermore, the effect of telithromycin against the biofilms of enterococci were investigated.Results: A total of 280 Enterococcus faecalis and 122 Enterococcus faecium isolates were collected from individual inpatients in China. Telithromycin showed excellent activity against the E. faecalis and E. faecium strains no matter sensitive or resistant to erythromycin with erm A, erm B or erm C, with the MIC50 at 2 μg/mL and 4 μg/mL, respectively. The predominant STs of E. faecalis isolates were ST16, ST30, ST179, and ST18, ST78, ST80 were the predominant STs of E. faecium isolates. Moreover, 87.1% (135/158) and 80.4% (41/51) isolates of the predominant STs carried the erm genes in E. faecalis and E. faecium isolates, respectively. The subinhibitory concentration of telithromycin (at 1/4× and 1/8× MICs) significantly inhibited the biofilm formation of 16 E. faecalis isolates. Telithromycin (at 8× MIC) indicated the removal effect on the established biofilms of 8 E. faecalis isolates, and combined with ampicillin eradicated more biofilms than telithromycin or ampicillin alone.Conclusion: Telithromycin showed an excellent activity against the planktonic cells of E. faecalis and E. faecium, and against the biofilms of E. faecalis.

show abstract

Presence of Vancomycin, Aminoglycosides, and Erythromycin Resistance Genes in Enterococci Isolated from Clinical Samples in Turkey

Cited by 7 publications

References 21 publications

High level aminoglycoside resistant enterococci inhospital-acquired urinary tract infections in Mansoura, Egypt

High level aminoglycoside resistant enterococci inhospital-acquired urinary tract infections in Mansoura, Egypt

Should we leave the paper currency? A microbiological examination

In vitro antibacterial activity of telithromycin against Enterococcus spp. isolated from China

Contact Info

Product

Resources

About