Parts of the Australian continent, including the Otway Basin of the southern Australian margin, exhibit unusually high levels of neotectonic deformation for a so-called stable continental region. The onset of deformation in the Otway Basin is marked by a regional Miocene-Pliocene unconformity and inversion and exhumation of the Cretaceous-Cenozoic basin fill by up to c. 1 km. While it is generally agreed that this deformation is controlled by a mildly compressional intraplate stress field generated by the interaction of distant plate-boundary forces, it is less clear whether the present-day record of deformation manifested by seismicity is representative of the longer-term geological record of deformation. We present estimates of strain rates in the eastern Otway Basin since 10 Ma based on seismic moment release, geological observations, exhumation measurements and structural restorations. Our results demonstrate significant temporal variation in bulk crustal strain rates, from a peak of c. 2 × 10 216 s 21 in the Miocene-Pliocene to c. 1.09 × 10 217 s 21 at the present day, and indicate that the observed exhumation can be accounted for solely by crustal shortening. The Miocene-Pliocene peak in tectonic activity, along with the orthogonal alignment of inverted post-Miocene structures to measured and predicted maximum horizontal stress orientations, validates the notion that plate-boundary forces are capable of generating mild but appreciable deformation and uplift within continental interiors.