Presentation of Candida albicans and purified protein derivative soluble antigens by Epstein-Barr virus-transformed human lymphoblastoid B-cell lines

Brown, Lawrence R.; Hank, Jacquelyn A.; Sondel, Paul M.

doi:10.1016/0008-8749(86)90228-5

Cited by 11 publications

(5 citation statements)

References 46 publications

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“…Although the role of B cells and antibodies in host defense against candidiasis has been studied extensively (1,12,14,18,32,35,38,39), clear understanding of their relative importance has not emerged (7,37,39). Some studies have demonstrated that passive transfer of immune serum can confer resistance to C. albicans experimentally in animals and humans (1,18,32).…”

Section: Discussionmentioning

confidence: 99%

“…However, data on the effect of T-lymphocyte-mediated systems in protection against invasive candidiasis do not establish as clear a role (2,6,10,11,14,16,20,25,26,30,40,41). Similarly, a role for a protective effect for B-lymphocyte-mediated mechanisms is not clearly established (1,12,16,18,32,35,39). Natural killer cells do not appear to have a major role in resistance to candidal infections (45,48).…”

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confidence: 99%

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Inoculation candidiasis in a murine model of severe combined immunodeficiency syndrome

et al. 1988

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To further elucidate the importance of T-and B-lymphocyte-mediated responses in host defense against systemic infection with Candida albicans, we studied this infection in a murine model of severe combined immunodeficiency (SCID). The course of inoculation candidiasis in these mice, which lack functional T and B lymphocytes, was compared with that in immunologically normal BALB/c mice. Mice were inoculated intravenously with 105 yeast cells. Quantitative cultures of liver, spleen, and kidneys were performed with necropsy specimens obtained 1, 3, 7, 10, 14, and 21 days after this intravenous inoculation. The differences in the time courses of recovery of organisms from liver and spleen specimens were not significantly different in the SCID mice compared with the BALB/c mice. The recovery of C. albicans from the kidneys was significantly lower in the SCID mice, indicating less persistence of the organism in the kidneys of the SCID mice than in those of the BALB/c mice. These data indicate that defense mechanisms other than Tand B-lymphocyte-mediated mechanisms are primarily responsible for host defense against inoculation candidiasis.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Inoculation candidiasis in a murine model of severe combined immunodeficiency syndrome

et al. 1988

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“…Specific antibodies against Candida albicans antigens have been described as protective [1][2][3][4][5], nonprotective [6][7][8][9], or facilitating [10] these infections. T lymphocytes do not seem relevant, because T cell-deficient mice are equally [11,12] or more [13] resistant to fungal growth than are normal mice, and the susceptibility of neutrophil-deficient mice (bg [beige]/bg nu [nude]/nu ϩ ) is not increased in congenitally athymic (bg/bg nu/nu) animals [14].…”

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confidence: 99%

Increased Resistance to Systemic Candidiasis in Athymic or Interleukin‐10–Depleted Mice

2000

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Candida albicans produces a virulence-associated immunomodulatory protein (p43), which activates lymphocytes polyclonally, suppresses specific antibody responses to Candida antigens, and potentiates systemic growth of C. albicans. In this study, athymic, unlike euthymic, C57BL/6 mice were resistant to systemic candidiasis and produced lower serum levels of interleukin (IL)-4 and IL-10. Pretreatment with p43 stimulated the production of both cytokines. Depletion of IL-10, but not of IL-4, in euthymic animals reestablished resistance and abrogated p43-dependent suppression of the specific antibody response and facilitated C. albicans growth. In agreement with these results, both immunosuppression and p43-mediated facilitation of the fungus growth were abolished in IL-10 knockout mice. These observations demonstrate the relevance of IL-10 in facilitating systemic candidiasis and suggest a critical role for the immunosuppressive virulence factor p43 in the process.

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“…This ability of lymphoblastoid cell lines to act as antigen-presenting cells was demonstrated to be due to their ability to bind and process tetanus toxoid, which was in contrast to some Burkitt's lymphoma lines that failed to process exogenous antigen (9). In contrast to these reports, another study showed that presentation of tetanus toxoid and Candida albicans soluble antigens to T cells by autologous lymphoblastoid cell lines was weaker than when peripheral blood mononuclear cells were used as antigen-presenting cells (32). There have been no reports as yet on the efficacy of presentation of P. gingivalis outer membrane antigens to T cells by autologous peripheral blood mononuclear cells and lymphoblastoid cell lines.…”

Section: Discussionmentioning

confidence: 86%

Apoptosis in Porphyromonas gingivalis–specific T‐cell lines

Gemmell

Prajaneh

Grieco

et al. 1999

Oral Microbiology and Immunology

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Fluorescence-activated cell sorter analysis and transmission electron microscopy were used to determine the presence of apoptotic cells in Porphyromonas gingivalis-specific T-cell lines established from the peripheral blood of 10 P. gingivalis-infected individuals. P. gingivalis outer membrane antigens were presented to the T cells by autologous Epstein-Barr virus-transformed B cells for 6, 24, 48 and 72 h. Transmission electron microscopy demonstrated the presence of typical apoptotic cells in all cultures. Annexin V-positive cells were present at low concentrations at all 4 four periods. A mean of approximately 2-3% of the CD4 cells and 1-3.5% of the CD8 cells were annexin V-positive, with an increase to around 5.5% positive CD4 cells at 6 h in wells containing P. gingivalis compared with cultures not containing antigen. This difference was not, however, significant at the 0.05 level (P = 0.073). The mean (+/- standard error) CD4:CD8 ratios of the T-cell lines when first established using peripheral blood mononuclear cells as antigen-presenting cells was significantly higher (5.2 +/- 1.1) than when transformed B cells were used as antigen-presenting cell (1.2 +/- 0.5). While this study has shown apoptosis occurring in the T-cell lines, it has not shown definitively that the reversion in the CD4:CD8 ratio in the P. gingivalis-specific T cells following antigen presentation by autologous Epstein-Barr virus-transformed B cells is due to apoptosis of a CD4 population. Alternatively, the reversion in the CD4:CD8 ratio could be due to a selective proliferation of the CD8 population which, in turn, could be relevant to the immunopathology of periodontal disease induced by P. gingivalis.

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Presentation of Candida albicans and purified protein derivative soluble antigens by Epstein-Barr virus-transformed human lymphoblastoid B-cell lines

Cited by 11 publications

References 46 publications

Inoculation candidiasis in a murine model of severe combined immunodeficiency syndrome

Inoculation candidiasis in a murine model of severe combined immunodeficiency syndrome

Increased Resistance to Systemic Candidiasis in Athymic or Interleukin‐10–Depleted Mice

Apoptosis in Porphyromonas gingivalis–specific T‐cell lines

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