2020
DOI: 10.1038/s41598-020-63308-w
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Presenting a codon-optimized palette of fluorescent proteins for use in Candida albicans

Abstract: Fluorescent proteins with varying colors are indispensable tools for the life sciences research community. These fluorophores are often developed for use in mammalian systems, with incremental enhancements or new versions published frequently. However, the successful application of these labels in other organisms in the tree of life, such as the fungus Candida albicans, can be difficult to achieve due to the difficulty in engineering constructs for good expression in these organisms. In this contribution, we p… Show more

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Cited by 11 publications
(12 citation statements)
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“…This opportunistic fungal pathogen can cause bloodstream infections, also called candidemia, which has a crude mortality rate of approximately 40% ( 1 ). Fluorescence imaging of this organism has been performed on both a macroscale ( 2 ) and a subcellular scale, although both approaches tend to make use of the overexpression of specific targets labeled with a range of fluorescent proteins (FPs) ( 3 6 ).…”
Section: Introductionmentioning
confidence: 99%
“…This opportunistic fungal pathogen can cause bloodstream infections, also called candidemia, which has a crude mortality rate of approximately 40% ( 1 ). Fluorescence imaging of this organism has been performed on both a macroscale ( 2 ) and a subcellular scale, although both approaches tend to make use of the overexpression of specific targets labeled with a range of fluorescent proteins (FPs) ( 3 6 ).…”
Section: Introductionmentioning
confidence: 99%
“…Candida enhanced ffDronpa and γmGFP expressing strains that have been used in the photoswitching assessment were published previously (4) and grown overnight on Low Fluorescent medium (LoFlo) containing 0.69%(w/v) Yeast Nitrogen Base (without amino acids, folic acid and riboflavin), 0.25%(w/v) ammonium sulphate, 0.079%(w/v) complete supplement mixture (CSM; MP Biomedicals) and 2%(w/v) glucose. The strains were brought to an optical density at 600 nm (OD 600 ) of 0.2 in fresh Low Fluorescent medium and grown to the exponential phase (4-5 hrs) before measurement of photoswitching.…”
Section: Methodsmentioning
confidence: 99%
“…Fluorescence imaging of this organism has been performed on both a macro scale (2) and on a subcellular scale, though both approaches tend to make use of overexpression of specific targets labeled with a range of fluorescent proteins (3)(4)(5)(6).…”
Section: Introductionmentioning
confidence: 99%
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