Preservation and Transplantation of Human Cadaver Kidneys

Belzer, Folkert O.; Schweizer, Robert T.; Hoffman, Robert M.; Kountz, Samuel L.

doi:10.1097/00007890-197209000-00013

Cited by 48 publications

(29 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Kidney preservation with continuous hypothermic perfusion is widely employed in clinical kidney transplantations [2,4,6,20]. There are rea sons to believe that the optimal conditions for this type of preservation have not yet been established, however.…”

mentioning

confidence: 99%

Fatty Acid and Glucose Utilization During Continuous Hypothermic Perfusion of Dog Kidney

Pettersson¹,

G²,

Scherstén³

1974

Eur Surg Res

View full text Add to dashboard Cite

The uptake and utilization of faty acids and glucose in dog kidneys subjected to continuous hypothermic perfusion were studied. 25 dog kidneys were perfused in a Gambro machine for 6 days. The concentrations of free fatty acids and glucose in the perfusate as well as the incorporation of labeled fatty acids and glucose into various metabolites in the kidney tissue and in the perfusate were determined. Linoleic, palmitic, and myristic acids were preferably incorporated into tissue glycerides, while caprylic acid was mainly incorporated into CO₂, glucose, and lactate. Labeled glucose was incorporated mainly into lactate, but a low activity was also recovered in CO₂ and in tissue glycogen. A continuous lactate production and an increase in the ratio between lactate and pyruvate were registered during the perfusion. The concentration of phospholipids and triglycerides remained unchanged during 6 days’ perfusion, while the concentration of cholesterol decreased significantly Te results indicate that dog kidneys subjected to continuous hypothermic perfusion preferably use short-chained fatty acids for oxidation and long-chained fatty acids for incorporation into tissue glycerides. The glycolysis seems to be partially blocked at different levels, resulting in a low oxidation rate and a high lactate production. The results also seem to indicate that metabolic studies of perfused kidneys may make it possible to improve the composition of the perfusate with the aim to keep kidneys in a good condition for clinical transplantation.

show abstract

mentioning

confidence: 99%

Fatty Acid and Glucose Utilization During Continuous Hypothermic Perfusion of Dog Kidney

Pettersson¹,

G²,

Scherstén³

1974

Eur Surg Res

View full text Add to dashboard Cite

show abstract

“…2 The present study was designed to define the effects on small bowel survival of hypothermic pulsatile perfusion with a bloodless perfusate and to determine reliable functional parameters of intestinal viability during storage.…”

mentioning

confidence: 99%

Two-to-Three Day Intestinal Preservation Utilizing Hypothermic Pulsatile Perfusion

Toledo‐Pereyra¹,

Simmons²,

Najarían³

1974

Annals of Surgery

View full text Add to dashboard Cite

SHORT SEGMENTS OF SMALL INTESTINE have been successfully stored without perfusion for 48 hours under hyperbaric and hypothermic conditions6 and by perfusion with oxygeniated blood for 18 hours.1 The entire organ has been preserved for five hours by perfusion with Ringer's lactate solutions.9Y10 No systematic studies of intestinal preservation have been carried out using those methods of hypothermic, pulsatile, bloodless perfusion which have been found to be so successful in renal preservation.2 The present study was designed to define the effects on small bowel survival of hypothermic pulsatile perfusion with a bloodless perfusate and to determine reliable functional parameters of intestinal viability during storage. MethodsTwenty adult mongrel dogs of both sexes weighing between 15 and 24 kg were used as small bowel donors. They were anesthetized with sodium methohexital and maintained on halothane. The small intestine from the third portion of the duodenum to the ileocecal valve was resected with its mesenteric blood supply. The superior mesenteric artery was cannulated and the small intestine flushed with a cold (4°C) Ringer's lactate solution containing heparin (10,000 U/L) and procaine (1 gm/L) until the venous effluent was clear. A catheter was inserted into both ends of the intestine to drain the intestinal secretions during perfusion. The small intestine

show abstract

“…Continuous hypothermic perfusion has proved to be a satisfactory method for necro-kidney preservation [1,3,6,10]. In our experience the Gambro machine has usually been safe and simple to operate.…”

Section: Discussionmentioning

confidence: 70%

“…Perfusion is per formed with cryoprecipitated plasma [1] or an albumin-electrolyte solu tion [3,6], and different machines have been constructed for this pur pose.…”

Section: Introductionmentioning

confidence: 99%

Gaseous Persufflation during Machine Perfusion of Human Kidneys before Transplantation

Flatmark¹,

Slaattelid²,

Woxholt³

1975

Eur Surg Res

View full text Add to dashboard Cite

Massive air embolisation (persufflation) of four machine-perfused human kidneys occurred during preservation. This lasted for 60–120 min and caused increasing perfusion resistance. Pressure and flow returned to normal within 2 h after reperfusion. All kidneys showed immediate function after transplantation. Experiments with dog kidneys revealed closure of cortical vessels and increased kidney temperature during persufflation. Cortical flow became normal after 1 h of reperfusion.

show abstract

Preservation and Transplantation of Human Cadaver Kidneys

Cited by 48 publications

References 0 publications

Fatty Acid and Glucose Utilization During Continuous Hypothermic Perfusion of Dog Kidney

Fatty Acid and Glucose Utilization During Continuous Hypothermic Perfusion of Dog Kidney

Two-to-Three Day Intestinal Preservation Utilizing Hypothermic Pulsatile Perfusion

Gaseous Persufflation during Machine Perfusion of Human Kidneys before Transplantation

Contact Info

Product

Resources

About