Preservation effects in geometric morphometric approaches: freezing and alcohol in a freshwater fish

Berbel‐Filho, Waldir M.; Jacobina, Uedson Pereira; Martínez, P.

doi:10.1007/s10228-013-0339-x

Cited by 26 publications

(32 citation statements)

References 7 publications

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“…Specimens were identified using morphological characteristics described by Bussing () and Castro‐Aguirre, Espinosa‐Perez, and Schmitter‐Soto (). After identification, selected fresh specimens were photographed to avoid deformation in size and shape caused by fixation and preservation procedures (Berbel‐Filho, Jacobina, & Martinez, ; Martinez, Berbel‐Filho, & Jacobina, ; Vergara‐Solana, García‐Rodríguez, & De La Cruz‐Agüero, ). Specimens, tissue samples, otoliths and urohyal bones used for molecular analysis and geometric morphometrics were catalogued and deposited in the Ichthyologic Collection of IPN–CICIMAR (http://coleccion.cicimar.ipn.mx), where they are available upon request.…”

Section: Methodsmentioning

confidence: 99%

Supporting the existence of two isolated evolutionary lineages ofGerres(Perciformes: Gerreidae) in America

et al. 2019

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For many years in America, many authors have considered that the Gerres genus contained a single species, the yellowfin mojarra Gerres cinereus (Walbaum, 1792); however, others have recognized two species in this genus in the Neotropics. In this study, specimens of G. cinereus (sensu lato) caught throughout its geographic range on both coasts of America were compared for the first time to analyse divergence among the sampled populations. A genetic analysis based on DNA sequences from three mitochondrial genes and one nuclear gene revealed a clear separation of two clades, one in the Pacific and one in the Atlantic. Geometric morphometric differences, based on body shape, sagittae otoliths and urohyal bone features, were significant between the two groups, including separation by an accurate classification a posteriori by a canonical variate analysis (CVA) > 82% in all data sets (overall mean >91%). Integration of the results of these approaches indicates that there is sufficient evidence to support species status for Gerres simillimus Regan, 1907, and it should be considered a valid species in the neotropical Pacific.

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Section: Methodsmentioning

confidence: 99%

Supporting the existence of two isolated evolutionary lineages ofGerres(Perciformes: Gerreidae) in America

et al. 2019

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“…Similarly, pressing and drying plant leaves can affect their shape noticeably [305] and may therefore have serious effects on studies of asymmetry. Many other types of specimens need to be stored in liquids such as ethanol or formalin, which changes tissue properties and also affects length measurements [306] and shape [307,308]. Even freezing can affect the shape of fish significantly [308].…”

Section: Preparation and Preservation Of Specimensmentioning

confidence: 99%

“…Many other types of specimens need to be stored in liquids such as ethanol or formalin, which changes tissue properties and also affects length measurements [306] and shape [307,308]. Even freezing can affect the shape of fish significantly [308]. Naturally, such artifacts from preservation are most serious for soft tissues such as embryos [309] or brain tissue [310].…”

Section: Preparation and Preservation Of Specimensmentioning

confidence: 99%

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Analyzing Fluctuating Asymmetry with Geometric Morphometrics: Concepts, Methods, and Applications

2015

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Approximately two decades after the first pioneering analyses, the study of shape asymmetry with the methods of geometric morphometrics has matured and is a burgeoning field. New technology for data collection and new methods and software for analysis are widely available and have led to numerous applications in plants and animals, including humans. This review summarizes the concepts and morphometric methods for studying asymmetry of shape and size. After a summary of mathematical and biological concepts of symmetry and asymmetry, a section follows that explains the methods of geometric morphometrics and how they can be used to analyze asymmetry of biological structures. Geometric morphometric analyses not only tell how much asymmetry there is, but also provide information about the patterns of covariation in the structure under study. Such patterns of covariation in fluctuating asymmetry can provide valuable insight about the developmental basis of morphological integration, and have become important tools for evolutionary developmental biology. The genetic basis of fluctuating asymmetry has been studied from empirical and theoretical viewpoints, but serious challenges remain in this area. There are many promising areas for further research that are only little explored at present.

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“…melanurus (Sidlauskas et al, 2006), Hyphessobrycon gr. heterorhabdus (García-Alzate et al, 2010), Aspidoras albater Nijssen, Isbrücker 1976 (Secutti et al, 2011), Cichla kelberi Kullander, Ferreira 2006(Berbel-Filho et al, 2013, Astyanax bimaculatus (Linnaeus 1758) (Santos, Araújo, 2015) and Brycon henni Eigenmann 1913 and Saccodon dariensis (Meek, Hildebrand 1913) (Restrepo-Escobar et al, 2016a;2016b). Regarding solely Amazonian fishes, even fewer studies have examined morphological variation in body shape and size, e.g., CoxFernandes (1998) on two apteronotid species, Sidlauskas et al (2011) on Leporinus cylindriformis group and Reiss, Grothues (2015) on Cichla temensis.…”

Section: Introductionmentioning

confidence: 99%

Morphological variation among populations of Hemigrammus coeruleus (Characiformes: Characidae) in a Negro River tributary, Brazilian Amazon

et al. 2017

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We explored patterns of phenotypic variation in Hemigrammus coeruleus from the Unini River basin, a blackwater river in the Brazilian Amazon. Geometric morphometrics was used to evaluate variation in body shape among populations from four tributaries (UN2-UN5). We found no evidence for sexual dimorphism in body size and shape. However, morphological differences among populations were detected as the analyses recovered significant groups corresponding to each sub-basin, with some overlap among them. The populations from UN2, UN3 and UN5 had more elongate bodies than fish from UN4. The most morphologically divergent population belonged to UN4, the tributary with the most divergent environmental conditions and the only one with seasonally-muddy waters. The morphological variation found among these populations is likely due to phenotypic plasticity or local adaptation, arising as a product of divergent ecological selection pressures among sub-basins. This work constitutes one of the first to employ a population-level geometric morphometric approach to assess phenotypic variation in Amazonian fishes. This method was able to distinguish subtle differences in body morphology, and its use with additional species can bring novel perspectives on the evaluation of general patterns of phenotypic differentiation in the Amazon.Keywords: Geometric morphometrics, Morphology, Ornamental fish, Phenotypic plasticity, Populations.Neste estudo foram explorados os padrões de variação fenotípica em Hemigrammus coeruleus da bacia do rio Unini, um rio de água preta na Amazônia brasileira. Métodos de morfometria geométrica foram aplicados para avaliar as variações na forma do corpo entre populações provenientes de quatro tributários (UN2-UN5). Os resultados mostraram ausência de dimorfismo sexual relacionado ao tamanho e formato do corpo. Entretanto, diferenças morfológicas entre populações foram detectadas, uma vez que as análises apontaram agrupamentos correspondendo a cada sub-bacia, com certo grau de sobreposição entre populações. As populações dos rios Preto, Arara e Pauini (UN2, UN3 e UN5) apresentaram formato de corpo mais alongado do que a amostra do igarapé Solimõezinho (UN4). A população mais divergente morfologicamente pertenceu ao igarapé Solimõezinho (UN4), o tributário que apresentou a condição ambiental mais divergente e o único com águas sazonalmente barrentas. A variação morfológica encontrada nessas populações de H. coeruleus é provavelmente devido a plasticidade fenotípica ou adaptação local por seleção induzida por diferentes pressões seletivas entre as sub-bacias. Este estudo constitui a primeira contribuição científica usando métodos de morfometria geométrica para avaliar a variação fenotípica entre populações em peixes amazônicos. Esse método foi capaz de distinguir diferenças sutis na morfologia e sua replicação em outras espécies amazônicas pode trazer novas perspectivas na avaliação de padrões gerais de diferenciação fenotípica na região.

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Preservation effects in geometric morphometric approaches: freezing and alcohol in a freshwater fish

Cited by 26 publications

References 7 publications

Supporting the existence of two isolated evolutionary lineages ofGerres(Perciformes: Gerreidae) in America

Supporting the existence of two isolated evolutionary lineages ofGerres(Perciformes: Gerreidae) in America

Analyzing Fluctuating Asymmetry with Geometric Morphometrics: Concepts, Methods, and Applications

Morphological variation among populations of Hemigrammus coeruleus (Characiformes: Characidae) in a Negro River tributary, Brazilian Amazon

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