Preservation of Bacillus firmus Strain 37 and Optimization of Cyclodextrin Biosynthesis by Cells Immobilized on Loofa Sponge

Pazzetto, Rúbia; Ferreira, Sabrina Barbosa de Souza; Santos, Elder James Silva; Moriwaki, Cristiane; Guedes, Terezinha Aparecida; Matioli, Graciette

doi:10.3390/molecules17089476

Cited by 12 publications

(10 citation statements)

References 29 publications

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“…PLC producers were maintained onto nutrient agar slants kept at 4°C subcultured every month. Glycerol stocking [26] and lyophilization [27] were used for the long term preservation of some selected isolates.…”

Section: Bacterial Strains Isolation and Maintenancementioning

confidence: 99%

Production and Characterization of Phospholipases C from some Bacillus thuringiensis Isolates Recovered from Egyptian Soil

Elleboudy

Elkhatib

Aboulwafa

et al. 2016

Int. J. Biotech. Well. Indus.

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Two hundred and thirty one isolates, with the characteristic morphology of Genus Bacillus, were recovered from 100 soil samples collected from 7 different Egyptian governorates, and were screened for phospholipase C (PLC) production by egg-yolk plate method. Sixty isolates have shown very high PLC production and were further assessed using chromogenic assay method. The highest five producers, identified by 16S rRNA gene sequencing as Bacillus thuringiensis, were selected and their PLCs were purified to homogeneity using ammonium sulfate precipitation and Sephadex G-75 gel filtration chromatography. PLCs had molecular masses of 28.5 kDa as indicated by SDS-PAGE. The characteristics of the studied five PLCs were having maximal activities at 35-45°C and pH 7.2. The enzymes could retain more than half of their maximum activities at 30-60°C and pH 7-8. Equivalent activities were recorded at low water tension. PLC from B. thuringiensis KT159186 was relatively thermostable with a maximum activity at 40°C. The halfinactivation temperature was above 50°C, which compared favorably to that of other enzymes. Activity at the wide temperature range (20-80°C) was high (about 50% of maximum),. This PLC could tolerate pH as high as 12 with only 30% loss of activity. Specificity pattern of PLC from the same isolate showed equivalent activities toward phosphatidylcholine and phosphatidylinositol in addition to marked activity toward phosphatidylethanolamine, which makes it a typical non-specific PLC for industrial purposes. In conclusion, these characteristics of PLC from the test isolate make it attractive for various industrial applications.

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Section: Bacterial Strains Isolation and Maintenancementioning

confidence: 99%

Production and Characterization of Phospholipases C from some Bacillus thuringiensis Isolates Recovered from Egyptian Soil

Elleboudy

Elkhatib

Aboulwafa

et al. 2016

Int. J. Biotech. Well. Indus.

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“…Given the variation in the carbon length of the molecules, it is plausible for the GBS to manifest antimicrobial activity against a broader spectrum of microorganisms with superior activity as compared to other GBSs [20]. It was, therefore, noteworthy that we achieved the optimum conditions for GBS production, given cultural factors that may affect the production [21].…”

Section: Discussionmentioning

confidence: 99%

Bioactivity of a Novel Glycolipid Produced by a Halophilic Buttiauxella sp. and Improving Submerged Fermentation Using a Response Surface Method

2016

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An antimicrobial glycolipid biosurfactant (GBS), extracted and identified from a marine bacterium, was studied to inhibit pathogenic microorganisms. Production of the GBS was optimized using a statistical method, a response surface method (RSM) with a central composite design (CCD) for obtaining maximum yields on a cost-effective substrate, molasses. The GBS-producing bacterium was identified as Buttiauxella Species in terms of biochemical and molecular characteristics. This compound showed a desirable antimicrobial activity against some pathogens such as E. coli, Bacillus subtilis, Bacillus cereus, Candida albicans, Aspergilus niger, Salmonella enterica. The rheological studies described the stability of the GBS at high values in a range of pH (7-8), temperature (20-60) and salinity (0%-3%). The statistical optimization of GBS fermentation was found to be pH 7, temperature 33 • C, Peptone 1%, NaCl 1% and molasses 1%. The potency of the GBS as an effective antimicrobial agent provides evidence for its use against food and human pathogens. Moreover, favorable production of the GBS in the presence of molasses as a cheap substrate and the feasibility of pilot scale fermentation using an RSM method could expand its uses in food, pharmaceutical products and oil industries.

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“…Pazzetto et al [52] evaluated CD production by immobilizing B. firmus strain no. 37 on a loofah sponge.…”

Section: Reaction Timementioning

confidence: 99%

“…Pazzetto et al [52] discovered that CD yield from B. firmus strain no. 37 immobilized on loofah sponge increased from 6.46 mM to 8.83 mM as the temperature was raised from 40 C to 60 C.…”

Section: Temperaturementioning

confidence: 99%

Cell Immobilization for Cyclodextrin Production: Mini Review

Baharudin¹,

Man²,

Manas

et al. 2023

Jurnal Teknologi

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Cell immobilization has been applied in various industries, including chemical manufacturing, food, pharmaceutical, and textile. Recently, innovations in cell immobilization techniques and support materials have been put forward for application in high value-added chemical biosynthesis, such as cyclodextrin (CD). The techniques, support materials, and process parameters of cell immobilization play important roles in achieving high CD yield. This review should help one choose the correct cell immobilization technique and support for a CD biosynthesis setup. Previously, CD biosynthesis utilized free cells, even though they present difficulties such as the low product yield, cell lysis, unstable plasmid, and non-reusable cells. This review highlights how the problems that arise from free-cell bioreactors could be mitigated by cell immobilization. The process conditions of cell immobilization for CD production are also presented.

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Preservation of Bacillus firmus Strain 37 and Optimization of Cyclodextrin Biosynthesis by Cells Immobilized on Loofa Sponge

Cited by 12 publications

References 29 publications

Production and Characterization of Phospholipases C from some Bacillus thuringiensis Isolates Recovered from Egyptian Soil

Production and Characterization of Phospholipases C from some Bacillus thuringiensis Isolates Recovered from Egyptian Soil

Bioactivity of a Novel Glycolipid Produced by a Halophilic Buttiauxella sp. and Improving Submerged Fermentation Using a Response Surface Method

Cell Immobilization for Cyclodextrin Production: Mini Review

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