Preservation of Biospecimens at Ambient Temperature: Special Focus on Nucleic Acids and Opportunities for the Biobanking Community

Müller, Rolf; Betsou, Fay; Barnes, Michael; Harding, Keith; Bonnet, Jacques; Kofanova, Olga; Crowe, John H.

doi:10.1089/bio.2015.0022

Cited by 30 publications

(23 citation statements)

References 76 publications

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“…The mid- and long-term stability of the quality of the samples conserved at low temperature is often difficult to maintain and control, requiring substantial precautions [ 52 , 53 , 54 ]. It is for this reason that several procedures have been developed to maintain the quality and stability at RT of nucleic acids over a long period of time for subsequent use in research [ 55 , 56 , 57 , 58 , 59 , 60 , 61 ].…”

Section: Discussionmentioning

confidence: 99%

Ensuring the Safety and Security of Frozen Lung Cancer Tissue Collections through the Encapsulation of Dried DNA

Washetine

Kara-Borni

Heeke

et al. 2018

Cancers

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Collected specimens for research purposes may or may not be made available depending on their scarcity and/or on the project needs. Their protection against degradation or in the event of an incident is pivotal. Duplication and storage on a different site is the best way to assure their sustainability. The conservation of samples at room temperature (RT) by duplication can facilitate their protection. We describe a security system for the collection of non-small cell lung cancers (NSCLC) stored in the biobank of the Nice Hospital Center, France, by duplication and conservation of lyophilized (dried), encapsulated DNA kept at RT. Therefore, three frozen tissue collections from non-smoking, early stage and sarcomatoid carcinoma NSCLC patients were selected for this study. DNA was extracted, lyophilized and encapsulated at RT under anoxic conditions using the DNAshell technology. In total, 1974 samples from 987 patients were encapsulated. Six and two capsules from each sample were stored in the biobanks of the Nice and Grenoble (France) Hospitals, respectively. In conclusion, DNA maintained at RT allows for the conservation, duplication and durability of collections of interest stored in biobanks. This is a low-cost and safe technology that requires a limited amount of space and has a low environmental impact.

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Section: Discussionmentioning

confidence: 99%

Ensuring the Safety and Security of Frozen Lung Cancer Tissue Collections through the Encapsulation of Dried DNA

Washetine

Kara-Borni

Heeke

et al. 2018

Cancers

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“…In this sense, fitness-for-purpose procedures should be validated and established when a prospective workflow is designed, taking into consideration the general good practices that do allow the most use of samples [2,12]. Independently, other approaches have been reviewed for the conservation of samples at room temperature by biobanks [25]. Before sending of samples to analytical laboratories from the storage facilities, a checkpoint is introduced to guarantee that each aliquot is compliant with the corresponding study or with the necessary volume of sample to thus supplement with additional aliquots.…”

Section: Monitored Storage Of Biological Samples and Distributionmentioning

confidence: 99%

Harmonized and Quality Sample Handling in Biobank-Supported Multicenter Prospective Studies

Valdivieso-Gómez¹,

Garrancho-Pérez²,

Aroca-Siendones³

et al. 2021

Human Blood Group Systems and Haemoglobinopathies

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In the frame of multicenter research studies, biobanks ensure the harmonization and traceability of the prospective collection of quality samples. This is significant because pre-analytical variables must be carefully considered to guarantee the integrity of biomarkers to be tested and to avoid bias affecting the validity of the analytical results. According to a quality management system, biobanks contribute with documents and records; consumable preparation for collection, processing, and conservation; sample quality controls; and centralized management of sample handling, storage, and distribution. Traceability of samples is based on unique standard codes and the use of pre-assigned, pre-coded, and pre-labeled materials for sample collection, processing, and conservation. By using these supporting tools, quality derivatives are obtained based on common and evidence-based standard operating procedures (SOPs), with associated traceability information in relation with their collection, processing, conservation, and distribution. The biobank-supported workflow, specifically designed and implemented for each project, allows obtaining harmonized quality samples contributing to the quality of large and complex research projects and the corresponding validity of the analyses.

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“…It is therefore necessary to improve nucleic acid sample collection to enable highly sensitive NAAT in RLS. It has been shown that extracted RNA stored in dry conditions shows less degradation and more consistent amplification, even when stored at room temperature or elevated temperatures over several weeks [20][21][22][23] . However, nucleic acid extraction from blood prior to shipment to central testing facility can be resource-intensive and impractical for RLS 24 .…”

Section: Introductionmentioning

confidence: 99%

SNAPflex: a paper-and-plastic device for instrument-free RNA and DNA extraction from whole blood

et al. 2020

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Preservation of Biospecimens at Ambient Temperature: Special Focus on Nucleic Acids and Opportunities for the Biobanking Community

Cited by 30 publications

References 76 publications

Ensuring the Safety and Security of Frozen Lung Cancer Tissue Collections through the Encapsulation of Dried DNA

Ensuring the Safety and Security of Frozen Lung Cancer Tissue Collections through the Encapsulation of Dried DNA

Harmonized and Quality Sample Handling in Biobank-Supported Multicenter Prospective Studies

SNAPflex: a paper-and-plastic device for instrument-free RNA and DNA extraction from whole blood

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