Preservation of high phenylalanine ammonia lyase activities in roots of Japanese Striped corn: A potential oral therapeutic to treat phenylketonuria

López‐Villalobos, Arturo; Lücker, Joost; López-Quiróz, Ana Angela; Yeung, Edward C.; Palma, Kristoffer; Kermode, Allison R.

doi:10.1016/j.cryobiol.2014.03.003

Cited by 3 publications

(7 citation statements)

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“…JS roots for the cereal bar were cut to 5 mm in length, immersed in pure maple syrup (35 g) (Camp Brand, Plessisville, QC, Canada) and stored overnight at 0.01 °C prior to incorporation into the product, whereas roots for the beverage and jam‐like spread were cut to ∼1–2 mm prior to incorporation into the products. Roots were added in the final stages of preparation of the products and were neither mixed at high speed nor baked, in order to retain their PAL enzyme activity.…”

Section: Methodsmentioning

confidence: 99%

“…Phenylalanine ammonia lyase (PAL; EC 4.3.1.5) is a universal enzyme of higher plants that catalyzes the deamination of phenylalanine (Phe) to trans ‐cinnamic acid and free ammonia; the PAL associated with the seedling roots of high‐anthocyanin corn cultivars has received attention as having potential in the treatment of the human metabolic disorder phenylketonuria (PKU) …”

Section: Introductionmentioning

confidence: 99%

“…One high‐anthocyanin corn cultivar is particularly noteworthy, the ornamental variety Japanese Striped corn ( Zea mays L. cv. japonica ) …”

Section: Introductionmentioning

confidence: 99%

“…5 -8 Phenylalanine ammonia lyase (PAL; EC 4.3.1.5) is a universal enzyme of higher plants that catalyzes the deamination of phenylalanine (Phe) to trans-cinnamic acid and free ammonia; the PAL associated with the seedling roots of high-anthocyanin corn cultivars has received attention as having potential in the treatment of the human metabolic disorder phenylketonuria (PKU). 9 PKU is an inherited metabolic disorder caused by deficient activity of phenylalanine hydroxylase (PAH; EC 1.14.16.1), the enzyme responsible for the disposal of excess amounts of the essential amino acid Phe by its conversion to tyrosine. A Phe-restricted diet is still the current treatment for PKU patients and must be implemented immediately at birth to avoid the irreversible mental retardation and behavioral problems caused by Phe over-accumulation.…”

Section: Introductionmentioning

confidence: 99%

“…japonica). 9 While the sensory characteristics are available on selected sprouts such as lentils, cowpeas, peas and soybeans, 33 -35 no such profiles have been generated for corn sprouts or corn seedling roots. Therefore the objective of this research was to (1) document the taste and texture characteristics of corn roots using sensory descriptive analysis, (2) formulate low-Phe food products which are suitable for the addition of corn roots and (3) evaluate the consumer acceptability of the untreated products of (2) with the same products to which corn roots have been added.…”

Section: Introductionmentioning

confidence: 99%

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Descriptive and hedonic analyses of low‐Phe food formulations containing corn (Zea mays) seedling roots: toward development of a dietary supplement for individuals with phenylketonuria

et al. 2015

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…One high‐anthocyanin corn cultivar is particularly noteworthy, the ornamental variety Japanese Striped corn ( Zea mays L. cv. japonica ) …”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Descriptive and hedonic analyses of low‐Phe food formulations containing corn (Zea mays) seedling roots: toward development of a dietary supplement for individuals with phenylketonuria

et al. 2015

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A ratio fluorescence method based on dual emissive gold nanoclusters for detection of biomolecules and metal ions

et al. 2022

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Development of a New of Flour With Reduced Phenylalanine Content for Phenylketonuria Patients

Büyükkurt¹,

Durak²,

Erbaş³

2018

Gıda

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ÖZFenilketonüri (FKU) hastalığının tedavisinde, fenilalanince kısıtlı diyet tedavinin temelini oluşturmaktadır. Bu nedenle; FKU hastalarının diyetlerinde önemli bir çeşitlilik oluşturmak için buğday unundan fenilalanin içeriği azaltılmış yeni bir un (FAUN) hazırlanması amaçlanmıştır. Bu amaçla, unda bulunan proteinler in vitro olarak hidroliz edilmiş ve amino asitlerin serbestleşmesi sağlanmıştır. Serbestleşebilen bu amino asitlerin içerisindeki fenilalaninin miktarını azaltmak için ise, hidrolizata fenilalanin amonyum liyaz (FAL) enzimi içeren mısır filizi ekstraktı uygulanmış ve sonrasında elde edilen ürün kurutularak tekrar un haline getirilmiştir. Araştırma sonucunda, in vitro hidroliz ile unun serbest fenilalanin içeriğinin yaklaşık 15 kat arttığı ve 6.09 μmol/sa.g FAL enzim aktivitesine sahip mısır filizi ekstraktı uygulamasının da unun serbest fenilalanin içeriğini yaklaşık %50 oranında azalttığı tespit edilmiştir. Ayrıca FAUN ile üretilen bisküvilerin, duyusal özellikleri 9 puanlık hedonik skalaya göre yapılan değerlendirmede 4.5 ve daha yüksek puanları aldığı tespit edilmiştir. Anahtar kelimeler: Fenilketonüri, fenilalanin, fenilalanin amonyum liyaz ABSTRACT Phenylalanine-restricted diet is the basis of treatment for phenylketonuria (PKU). Therefore; it was aimed to prepare new flour with reduced phenylalanine (FRP) content from wheat flour in order to create a significant diversity in PKU patients' diet. For this purpose, proteins in flour were hydrolyzed by in vitro and amino acids were released. In order to reduce the amount of phenylalanine in these free-amino acids, corn seedling extracts containing phenylalanine ammonium lyase (PAL) was applied to hydrolysate, the resulting product was dried and obtained again flour. As a result of the research, it was determined that free phenylalanine content of flour was increased about 15-fold by in vitro and application of corn seedling extract with PAL enzyme activity of 6.09 μmol/h.g reduced about 50% of free phenylalanine content of flour. It was also determined that biscuits produced with FRP had sensorial scores of 4.5 or higher, based on the hedonic scale of 9 points.

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Preservation of high phenylalanine ammonia lyase activities in roots of Japanese Striped corn: A potential oral therapeutic to treat phenylketonuria

Cited by 3 publications

References 41 publications

Descriptive and hedonic analyses of low‐Phe food formulations containing corn (Zea mays) seedling roots: toward development of a dietary supplement for individuals with phenylketonuria

Descriptive and hedonic analyses of low‐Phe food formulations containing corn (Zea mays) seedling roots: toward development of a dietary supplement for individuals with phenylketonuria

A ratio fluorescence method based on dual emissive gold nanoclusters for detection of biomolecules and metal ions

Development of a New of Flour With Reduced Phenylalanine Content for Phenylketonuria Patients

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