2011
DOI: 10.1159/000326632
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Preservation of Human Cornea

Abstract: The successful outcome of the majority of corneal transplants depends on the presence of a viable corneal endothelium. This monolayer of cells lines the inner surface of the cornea and its primary function is to maintain corneal transparency by controlling the hydration of the collagenous stromal layer. Since human corneal endothelial cells do not readily proliferate, preservation of the endothelium is a primary aim of methods of corneal storage. Although some cryopreserved corneas have been transplanted succe… Show more

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Cited by 123 publications
(115 citation statements)
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“…60,61 We here compared three different cooling rates (0.1°C, 1°C and 5°C/min) and observed better results for solutions 1–3 than for cell culture medium with all three. Surprisingly, in contrast to previous publications on immortalized human endothelial cell monolayers 60 and corneal keratinocytes, 62 best results for porcine aortic endothelial cell monolayers were not achieved with the slowest cooling rate (0.1°C/min; Figure 6A), but with a standard cooling rate of −1°C/min (Figure 6B). Von Bomhard et al also produced good results with endothelial cell suspensions in a modified TiProtec solution in vitrification experiments (plunging in liquid nitrogen).…”
Section: Discussioncontrasting
confidence: 99%
“…60,61 We here compared three different cooling rates (0.1°C, 1°C and 5°C/min) and observed better results for solutions 1–3 than for cell culture medium with all three. Surprisingly, in contrast to previous publications on immortalized human endothelial cell monolayers 60 and corneal keratinocytes, 62 best results for porcine aortic endothelial cell monolayers were not achieved with the slowest cooling rate (0.1°C/min; Figure 6A), but with a standard cooling rate of −1°C/min (Figure 6B). Von Bomhard et al also produced good results with endothelial cell suspensions in a modified TiProtec solution in vitrification experiments (plunging in liquid nitrogen).…”
Section: Discussioncontrasting
confidence: 99%
“…The aim of this approach is to minimize or even inhibit cell metabolism to preserve the original properties of the cornea (Armitage 2011). During storage, the cornea remains thin and is directly available for surgery.…”
Section: Discussionmentioning
confidence: 99%
“…Moist pot storage, first described in 1935, is limited by the rapid deterioration of donor tissue over the initial 24-48 h following retrieval (Armitage 2011). From the 1970s, additional options for corneal storage provided increasing benefits.…”
mentioning
confidence: 99%
“…Organ Culture also allows the acceptance of septic donors that are usually excluded from hypothermic storage (Spelsberg et al 2002). The additional assessment of the donor tissues prior to the surgery provides increased safety and quality control (Armitage 2011;Pels 1997;Borderie et al 1999). Perhaps the greatest practical benefit attributed to the extension of storage time is the ability to effectively plan surgery and identify donor tissue requirements to meet both scheduled and emergency demands (Pels 1997;Pollock and Moffatt 2010).…”
mentioning
confidence: 99%