Preservation of Neisseria gonorrhoeae : should swabs be refrigerated or not?

Serra-Pladevall, Judit; Blanco, Carlos Gulin; Olmo, Neus Vila; Camacho, Pilar Arjona; Domingo, Antonia Andreu

doi:10.1016/j.mimet.2017.12.012

Cited by 10 publications

(3 citation statements)

References 9 publications

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“…The study confirmed that refrigerated samples, which were stored in the non-nutritive transport media during 48 h, 72 h and 96 h had positive culture results more frequently than those stored at ambient condition, i.e. 73.9% vs 52.2%, 58.7% vs 37%, 47.8% vs 17.4% respectively, which is in line with Serra-Pladevall J et al ( 23 ). The best viability rate was for refrigerated transport media during 24 h (93.5%) and 48 h (73.9%).…”

Section: Discussionsupporting

confidence: 88%

Comparison of deferred and bedside culture of Neisseria gonorrhoeae: a study to improve the isolation of gonococci for antimicrobial susceptibility testing

Boiko¹,

Stepas²,

Krynytska³

2020

IJM

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Background and Objectives: Antimicrobial resistance of Neisseria gonorrhoeae is globally spread and threatening. Cultur- ing of N. gonorrhoeae is the only method to collect live isolates for investigation antimicrobial resistance profile. Therefore, quality assessment of N. gonorrhoeae culture is essential for successful isolation of gonococci. This study was conducted to evaluate deferred and bedside culture of N. gonorrhoeae depending on the year season and temperature condition of transport media temporary storage. Materials and Methods: Urogenital swabs from 46 symptomatic heterosexual patients with gonorrhoea and subculture of N. gonorrhoeae in 46 suspensions in concentrations 1.5 × 108 CFU/ml were subjected to the study. Non-nutritive transporting medium Amies Agar Gel Medium with charcoal (Copan Diagnostics Inc., Brescia, Italy) was used for deferred culture and selective Chocolate agar TM+PolyViteX VCAT3 (BioMérieux, Marcy-l'Étoile, France) for both tested methods of culture. Results: The specificity of both bedside and deferred methods of culture was 100%. The sensitivity of deferred culture was higher than of bedside culture (82.6% vs 47.8%, p<0.0005). Deferred culture showed significantly higher sensitivity com- paring to bedside culture in summer (100% vs 50%, p=0.003), and comparably the same as for bedside culture in autumn, winter and spring. Conclusion: The viability of N. gonorrhoeae subcultures was significantly higher in refrigerated samples from transport media than from ambient one after exposition from 48 to 96 hours. Optimal viability of N. gonorrhoeae was observed when transport swabs were kept refrigerated up to 48 h (73.9-93.5%) or ambiently – up to 24 h (87%). Updating laboratory guide- lines regarding sampling and timely specimen processing might improve gonococcal culture performance.

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Section: Discussionsupporting

confidence: 88%

Comparison of deferred and bedside culture of Neisseria gonorrhoeae: a study to improve the isolation of gonococci for antimicrobial susceptibility testing

Boiko¹,

Stepas²,

Krynytska³

2020

IJM

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“…In this study, we did not detect the presence of this bacterium, which could be because the type of sample used, in this case, urine, was not as significant as another type of sample, such as a urethral exudate. It is essential to emphasize that this bacterium does not survive more than 24 h at room temperature, and that a unique transport medium is needed [18]. In this study, the sample was at 4 • C, and Amies liquid medium with flocked swabs was used.…”

Section: Prevalence Comparisonmentioning

confidence: 99%

“…Hence, it is possible to attribute that they have already received prior treatment. However, it is known that infection by N. gonorrhoeae can be transient and relapse, despite being treated previously [18]. M. genitalium is detected more frequently in populations with risk factors such as the number of sexual partners throughout their lives, reaching up to 5% in males [25].…”

Section: Public Health Implicationsmentioning

confidence: 99%

Sexually Transmitted Infections in Male Patients with Urethritis

Llaca-Díaz,

Medina-Loredo,

Huerta-López

et al. 2023

Pathogens

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(1) Background: Sexually Transmitted Infections (STIs) in men are a significant public health problem due to the consequences they can have, such as chronic diseases, infertility, cancer, and even death. This study aimed to determine the frequency of microorganisms associated with STIs in men with urethritis attending urology consultations, and to explore their clinical correlations. (2) Methods: A population that attended the urology consultation of the University Hospital “Dr. José E. González” was studied. Written consent was obtained, and interviews and clinical history were conducted about specific risk factors identifying signs and symptoms associated with any genitourinary condition; after that, urine samples were collected. Identification of C. trachomatis, N. gonorrhoeae, U. urealyticum, and M. genitalium was based on amplifying species-specific DNA fragments. (3) Results: A total of 200 patients were included. The mean age was 55 years (20–95). According to the interviews, only 32.5% (n = 65) had received prior sex education. Additionally, 75% (n = 150) do not usually use any protection during sexual intercourse. Regarding clinical factors, 69.4% (n = 138) presented burning or pain when urinating. Molecular analysis revealed the presence of C. trachomatis to be 9.5% (n = 19), with U. urealyticum at 13% (n = 26), and M. genitalium at 2% (n = 4). (4) Conclusions: This is the most extensive molecular epidemiological study of the frequency of STIs in men in Mexico in third-level care and its association with different risk factors. As reported globally, a similar frequency of C. trachomatis, U. urealyticum, and M. genitalium was detected.

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Neisseria

Lâm,

Vogel,

Claus

2023

ClinMicroNow

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Microaerobic growth by denitrification of nitrite via nitric oxide has also been shown for Neisseria meningitidis . The species N. gonorrhoeae and N. meningitidis are exclusively human pathogens. Identification of the serogroup of invasive N. meningitidis isolates is of interest with respect to vaccination of close contacts of the index case. A further level of differentiation to analyze local epidemiology is achieved by DNA sequence‐based typing schemes of hypervariable outer membrane proteins, which have been harmonized throughout Europe. Cultivation of N. gonorrhoeae is best performed on chocolate agar, which supports the growth of many other commensal bacteria. DNA‐based methods used for typing of N. gonorrhoeae include gel‐based methods such as opa typing and restriction fragment length polymorphism analysis by pulsed‐field gel electrophoresis. Serologic tests are used for the determination of protection against invasive meningococcal disease after vaccination or for seroepidemiological studies. First‐catch urine is recommended for the molecular detection of genitourinary N. gonorrhoeae infections in men.

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Preservation of Neisseria gonorrhoeae : should swabs be refrigerated or not?

Cited by 10 publications

References 9 publications

Comparison of deferred and bedside culture of Neisseria gonorrhoeae: a study to improve the isolation of gonococci for antimicrobial susceptibility testing

Comparison of deferred and bedside culture of Neisseria gonorrhoeae: a study to improve the isolation of gonococci for antimicrobial susceptibility testing

Sexually Transmitted Infections in Male Patients with Urethritis

Neisseria

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