Preservation of the incretin effect after orthotopic pancreas transplantation in inbred rats

Kißler, Hermann; Gepp, H.; Schmiedl, A.; Schwille, P. O.

doi:10.1016/s0026-0495(99)90065-3

Cited by 7 publications

(4 citation statements)

References 38 publications

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“…Rats later on subjected to OGTT or MTT received indwelling silastic catheters (ThomafluidSilikon-Hochtemperatur-Chemieschlauch High Flexible, 0.5 -mm inner diameter Â 0.9-mm outer diameter and 1.0-mm inner diameter Â 1.4-mm outer diameter for vascular and oral insertion, respectively) in the left carotid artery, left jugular vein, and in the mouth [29]; in those rats subjected to undergo the euglycemic hyperinsulinemic clamp study, silastic catheters were implanted in the left carotid artery and both jugular veins.…”

Section: Implantation Of Indwelling Cathetersmentioning

confidence: 99%

“…One week after insertion of the catheters, when the rats returned to a normal body weight gain, the first OGTT was done in 8 animals of the sham and 13 animals of the Tx group as previously described [29]. Briefly, at 8 pm, after 12 hours of food deprivation, glucose 2 g/kg body weight was infused as 50% solution via the intraoral catheter over 1.5 minute by pump (model 5003; Precidor Infors, Basel, Switzerland).…”

Section: Oral Glucose Tolerance Testmentioning

confidence: 99%

“…At time 0, a continuous infusion of porcine insulin (Insulin S; Hoechst, Frankfurt am Main, Germany) was administered at time 0 to 130 minutes. The insulin infusion rate varied over a 10-fold range (14,22,29,86, and 129 pmol kg -1 d min -1 ) to increase the plasma insulin concentration to approximately 574, 718, 1292, 5023, and 10763 pmol/L in the respective clamp studies. A variable infusion of 25% glucose was started at time 0 and adjusted to clamp the plasma glucose concentration at 5.8 mmol/L.…”

Section: Euglycemic Clamp Studymentioning

confidence: 99%

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Glucose and lipid metabolism after liver transplantation in inbred rats: consequences of hepatic denervation

et al. 2005

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Section: Implantation Of Indwelling Cathetersmentioning

confidence: 99%

Section: Oral Glucose Tolerance Testmentioning

confidence: 99%

Section: Euglycemic Clamp Studymentioning

confidence: 99%

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Glucose and lipid metabolism after liver transplantation in inbred rats: consequences of hepatic denervation

et al. 2005

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“…Nineteen months after pancreas transplantation or sham-laparotomy, the rats were anaesthetized with ether, and indwelling silastic catheters were inserted into both jugular veins and into the mouth [22].…”

Section: Animals and Surgerymentioning

confidence: 99%

High insulin and low IGF-I plasma levels following pancreas transplantation in rats. Implications for bone and mineral metabolism

Hofmann²,

Gepp³

et al. 2000

Scandinavian Journal of Clinical and Laboratory Investigation

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Primary disturbances in mineral metabolism and deficiencies in insulin and insulin-like growth factor-I (IGF-I) have been implicated in the pathogenesis of diabetic osteopenia. This prompted us to investigate whether normal bone minerals and bone morphology are preserved after pancreas transplantation. To this end, 8 inbred rats (transplants) were compared with 9 sham-operated rats (controls) 20 months after orthotopic pancreas transplantation. While basal levels of insulin remained unaffected by transplantation, an oral glucose load elicited hyperinsulinemia (integrated incremental response: mean +/- SEM, 62+/-8 nmol l(-1) 60 min in transplants vs. 32+/-6 nmol l(-1) 60 min in controls; p<0.01) in the presence of normal glucose levels. Fecal and urinary excretion and fractional intestinal absorption of calcium, magnesium and phosphorus, net calcium absorption and the respective serum mineral levels were unchanged after transplantation, as were those of the calciotropic hormones. Serum osteocalcin and bone alkaline phosphatase remained unaffected, and urinary excretion of pyridinium and deoxypyridinium were unchanged. Fasting plasma IGF-I concentration was significantly decreased in transplants (930+/-42 ng ml(-1)) vs. control rats (1074+/-49 ng ml(-1); p < 0.05). Despite similar physical and chemical properties of bone in both groups, histomorphometry revealed slight osteopenia in transplant rats, as reflected by a 38% reduction in the cancellous bone area of the proximal tibial metaphysis. Plasma IGF-I levels were significantly correlated with bone mineral apposition rate (r=0.70, p<0.02), osteoblast perimeter (r=0.60, p<0.05) and osteoid perimeter (r=0.60, p<0.05). In conclusion, pancreas transplantation preserves physical and chemical properties of bone, but bone metabolism is not completely normal after transplantation, as evidenced by decreased cancellous bone. This might have resulted from the insulin resistance associated with the lowering of the plasma IGF-I level, which was correlated with the mineral apposition rate.

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Experimental Pancreas Transplantation

Farney¹,

Prieto²,

Cohen³

et al. 2004

Transplantation of the Pancreas

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Preservation of the incretin effect after orthotopic pancreas transplantation in inbred rats

Cited by 7 publications

References 38 publications

Glucose and lipid metabolism after liver transplantation in inbred rats: consequences of hepatic denervation

Glucose and lipid metabolism after liver transplantation in inbred rats: consequences of hepatic denervation

High insulin and low IGF-I plasma levels following pancreas transplantation in rats. Implications for bone and mineral metabolism

Experimental Pancreas Transplantation

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