2010
DOI: 10.1167/iovs.09-4109
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Preservation of the Limbal Stem Cell Phenotype by Appropriate Culture Techniques

Abstract: The proposed culture system supports enrichment and survival of limbal stem and progenitor cells during the entire cultivation process and may be essential for long-term restoration of the damaged ocular surface.

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Cited by 100 publications
(90 citation statements)
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“…17 Here, we demonstrated that primary LECs that did not receive UVA exhibited a cobblestone-like morphology. The cells were small and organized in tightly packed colonies, which expressed the markers P63a 34 and cytokeratin 15 35 which are associated with putative limbal stem cells and transient amplifying cells. The nonirradiated cells also were positive for the basal epithelial marker b1 integrin.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…17 Here, we demonstrated that primary LECs that did not receive UVA exhibited a cobblestone-like morphology. The cells were small and organized in tightly packed colonies, which expressed the markers P63a 34 and cytokeratin 15 35 which are associated with putative limbal stem cells and transient amplifying cells. The nonirradiated cells also were positive for the basal epithelial marker b1 integrin.…”
Section: Discussionmentioning
confidence: 99%
“…To identify changes in the phenotype of limbal epithelial cells following different treatments a panel of markers associated with different levels of cellular differentiation within the limbal epithelium has been used, namely integrin b1, p63a, cytokeratin (K)15, and cytokeratin (K)3. b1-Integrin, a marker normally observed in the basal and some of the suprabasal limbal epithelium, 33 the transcription factor p63a, 34 and K15, 35 are associated with limbal stem and progenitor cells and are absent in the superficial layers where the epithelial cells are considered terminally differentiated. On the other hand, K3 is expressed only in the superficial layers and is completely absent from basal and suprabasal cells.…”
Section: Immunocytochemistrymentioning
confidence: 99%
“…The cells were thawed and cultured as described by the manufacturer. LECs were maintained in culture media with a low calcium concentration (30 mM) as previously described (26).…”
Section: Cell Isolation and Cell Culturementioning
confidence: 99%
“…ISSN: proliferative potential, and percentage of cells presenting markers associated with stemness [20][21][22][23][24][25][26][27]. While optimal results for clinical purposes reportedly depend on adherence to the above outlined protocol [4], the results from experimental studies may indicate an adverse effect of trypsin-EDTA on viability [25], and on the proliferative potential of the dissociated cells [23,27], and such dissociation may also render the cells dependent on animal feeder cells for proper colony forming efficiency.…”
Section: Page -02mentioning
confidence: 99%