Preservation of the porcine pancreas with HTK and Euro‐Collins solution: studies in a reperfusion system

Leonhardt, U.; Barthel, M.; Tytko, A; Dröge, Michael; Siegel, E. G.; Nebendahl, Klaus; Köhler, H.; Creutzfeldt, W.

doi:10.1111/j.1365-2362.1990.tb01923.x

Cited by 4 publications

(1 citation statement)

References 12 publications

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“…Currently among others, different preservation solutions including University of Wisconsin (UW) solution [6], Celsior + [7,8], and Histidine-Tryptophane-Ketoglutarate (HTK) [9][10][11] among others are routinely used for pancreas preservation. A large number of reports have shown that the two-layer method (TLM), which employs oxygenated perfluorochemical (PFC) and UW solution, is superior to simple cold storage in UW [9,12].…”

Section: Introductionmentioning

confidence: 99%

Hyperbaric oxygenation of UW solution positively impacts on the energy state of porcine pancreatic tissue*

et al. 2011

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Background: Pancreatic islet transplantation is a promising option for the treatment of diabetic patients; xenotransplantation of porcine islet cells would be a possibility to overcome the shortage of donor organs. Usually the donor pancreas is preserved with University of Wisconsin (UW) solution. A large number of reports have shown that the two-layer method (TLM), which employs oxygenated perfluorochemical and UW solution, is superior to simple cold storage. However, the extensive use of TLM is cost intensive and there is evidence that TLM only oxygenates small parts of the organ preserved. Another possibility to increase the oxygen supply during organ preservation would be the use of hyperbaric oxygenation (HBO) which increases the oxygen tension in fluids. The aim of this study was to evaluate the effect of pre-oxygenation of different preservation solutions on organ quality in terms of high energy phosphate levels as well as the occurrence of apoptosis and the induction of heat shock proteins and nitrosative stress induced cell death in porcine pancreatic tissue.Methods: Porcine pancreatic tissue was preserved in different preservation solutions with or without pre-oxygenation for 6 hours of cold ischemic time (CIT). Then, tissue specimens were harvested and high energy phosphate levels were determined by HPLC. Moreover, immunohistochemistry was performed in order to detect occurrence of apoptosis, heat shock protein 70 (HSP70) as well as nitrosative stress induced cell death.Results: Organs stored in pre-oxygenated UW solution showed best results in terms of high energy phosphate levels; apoptotic cells per islet as well as HSP70 positivity were significantly less when compared to simple UW storage and all other organ preservation solutions with or without pre-oxygenation.Conclusions: Pre-oxygenation of UW solution is a simple and promising method to improve islet cell quality after cold organ storage. However, further in vitro experiments have to be performed in order to confirm these findings.

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Section: Introductionmentioning

confidence: 99%

Hyperbaric oxygenation of UW solution positively impacts on the energy state of porcine pancreatic tissue*

et al. 2011

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A stirred microchamber for oxygen consumption rate measurements with pancreatic islets

Papas

Pisania

et al. 2007

Biotech & Bioengineering

102

106

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Improvements in pancreatic islet transplantation for treatment of diabetes are hindered by the absence of meaningful islet quality assessment methods. Oxygen consumption rate (OCR) has previously been used to assess the quality of organs and primary tissue for transplantation. In this study, we describe and characterize a stirred microchamber for measuring OCR with small quantities of islets. The device has a titanium body with a chamber volume of about 200 µL and is magnetically stirred and water jacketed for temperature control. Oxygen partial pressure (pO 2 ) is measured by fluorescence quenching with a fiber optic probe, and OCR is determined from the linear decrease of pO 2 with time. We demonstrate that measurements can be made rapidly and with high precision. Measurements with βTC3 cells and islets show that OCR is directly proportional to the number of viable cells in mixtures of live and dead cells and correlate linearly with membrane integrity measurements made with cells that have been cultured for 24 h under various stressful conditions.

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Exocrine, but not endocrine, tissue is susceptible to microvascular ischemia/ reperfusion injury following pancreas transplantation in the rat

Vollmar¹,

Janata²,

Yamauchi³

et al. 1999

Transplant Int

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Preservation of the porcine pancreas with HTK and Euro‐Collins solution: studies in a reperfusion system

Cited by 4 publications

References 12 publications

Hyperbaric oxygenation of UW solution positively impacts on the energy state of porcine pancreatic tissue*

Hyperbaric oxygenation of UW solution positively impacts on the energy state of porcine pancreatic tissue*

A stirred microchamber for oxygen consumption rate measurements with pancreatic islets

Exocrine, but not endocrine, tissue is susceptible to microvascular ischemia/ reperfusion injury following pancreas transplantation in the rat

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