Preservation of vasoactive properties of human retinal arteries after cryopreservation

Abstract

The response to constrictive (endothelin-I) and vasodilatory agents (nimodipine and betaxolol) was comparable with that in fresh pig vessels. It is now possible to obtain multiple segments of retinal artery from a single donor eye and to preserve them for detailed pharmacological assessment.

An experimental study of VEGF induced changes in vasoactivity in pig retinal arterioles and the influence of an anti-VEGF agent

“…In the present study we investigate the vasoactive properties of VEGF in an isolated perfused porcine retinal arteriole preparation. Porcine retinal arteries have been shown to exhibit similar vasoactive properties to human retinal arteries with a range of vasoactive agents [15,16]. …”

Vasoconstrictive Effects of Sodium Fluorescein on Retinal Vessels Is Increased by Light Exposure

“…The vasoactive effects of sodium fluorescein seen in the current study are therefore observed with sodium fluorescein levels considerably lower than that expected in fluorescein angiography. As we have previously demonstrated comparable vasoactive effects of other agents in pig and human vessels, 16,17 it may be reasonable to expect sodium fluorescein to have contractile effects on human vessels. If this is the case, then reducing illumination level may limit any vasoconstrictive effects of sodium fluorescein when used in routine ophthalmic examinations.…”