Preservation of zebrafish genetic resources through testis cryopreservation and spermatogonia transplantation

Marinović, Zoran; Li, Qian; Lujić, Jelena; Iwasaki, Yoshiko; Csenki, Zsolt; Urbányi, Béla; Yoshizaki, Goro; Horváth, Ákos

doi:10.1038/s41598-019-50169-1

Cited by 42 publications

(22 citation statements)

References 38 publications

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“…First and foremost, we do not know the maximal length of time that sperm can remain well preserved under these conditions. Based on other reports 24 , we anticipate preservation could last many years. We used sperm densities of ca.…”

Section: Discussionmentioning

confidence: 87%

Sperm cryopreservation and in vitro fertilization techniques for the African turquoise killifish Nothobranchius furzeri

Dolfi

Suen

Ripa

et al. 2021

Sci Rep

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Over the last decade, the African turquoise killifish, Nothobranchius furzeri, has emerged as an important model system for the study of vertebrate biology and ageing. Propagation of laboratory inbred strains of Nothobranchius furzeri, such as GRZ, however, can pose challenges due to the short window of fertility, the efforts and space requirements involved in continuous strain maintenance, and the risks of further inbreeding. The current method for long term strain preservation relies on arrest of embryos in diapause. To create an alternative for long term maintenance, we developed a robust protocol to cryopreserve and revive sperm for in vitro fertilization (IVF). We tested a variety of extender and activator buffers for sperm IVF, as well as cryoprotectants to achieve practical long-term storage and fertilization conditions tailored to this species. Our protocol enabled sperm to be preserved in a cryogenic condition for months and to be revived with an average of 40% viability upon thawing. Thawed sperm were able to fertilize nearly the same number of eggs as natural fertilization, with an average of ~ 25% and peaks of ~ 55% fertilization. This technical advance will greatly facilitate the use of N. furzeri as a model organism.

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Section: Discussionmentioning

confidence: 87%

Sperm cryopreservation and in vitro fertilization techniques for the African turquoise killifish Nothobranchius furzeri

Dolfi

Suen

Ripa

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Based on other reports 28 , we anticipate preservation could last many years. We used sperm densities of ca.…”

Section: Discussionmentioning

confidence: 87%

Sperm cryopreservation and in vitro fertilization techniques for the African turquoise killifish Nothobranchius furzeri

Dolfi

Suen

Ripa

et al. 2021

Preprint

View full text Add to dashboard Cite

Over the last decade, the African turquoise killifish, Nothobranchius furzeri, has emerged as an important model system for the study of vertebrate biology and ageing. N. furzeri propagation, however, can pose challenges due to the short window of fertility, the efforts and space requirements involved in continuous strain maintenance, and the risks of inbreeding. The current method for long term strain preservation relies on arrest of embryos in diapause. Though useful, it remains unclear how long embryos can survive in this state. To create an alternative for long term maintenance, we developed a robust protocol to cryopreserve and revive sperm for in vitro fertilization (IVF). We tested a variety of extender and activator buffers for sperm IVF, as well as cryoprotectants to achieve feasible long-term storage and fertilization conditions tailored to this species. Our protocol enabled sperm to be preserved in a cryogenic condition for months and to be revived an average of 40% viability upon thawing. Thawed sperm were able to fertilize nearly the same number of eggs as natural fertilization, with an average of ~25% and peaks of ~55% fertilization. This technical advance will greatly facilitate the use of N. furzeri as a model organism.

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“…3). SDS mating is particularly amenable to species in which the embryo develops ex utero, such as birds, fish, amphibians and reptilian species [40][41][42][43] . As the chicken is a model organism for the study of embryogenesis, neural development and immunity in bird species, this technique offers a reliable platform for investigating gene function and disease resistance in poultry.…”

Section: Discussionmentioning

confidence: 99%

Direct allele introgression into pure chicken breeds using Sire Dam Surrogate (SDS) mating

et al. 2021

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Poultry is the most abundant livestock species with over 60 billion chickens raised globally per year. The majority of chicken are produced from commercial flocks, however many indigenous chicken breeds play an important role in rural economies as they are well adapted to local environmental and scavenging conditions. The ability to make precise genetic changes in chicken will permit the validation of genetic variants responsible for climate adaptation and disease resilience, and the transfer of beneficial alleles between breeds. Here, we generate a novel inducibly sterile surrogate host chicken. Introducing donor genome edited primordial germ cells into the sterile male and female host embryos produces adult chicken carrying only exogenous germ cells. Subsequent direct mating of the surrogate hosts, Sire Dam Surrogate (SDS) mating, recreates the donor chicken breed carrying the edited allele in a single generation. We demonstrate the introgression and validation of two feather trait alleles, Dominant white and Frizzle into two pure chicken breeds using the SDS surrogate hosts.

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Preservation of zebrafish genetic resources through testis cryopreservation and spermatogonia transplantation

Cited by 42 publications

References 38 publications

Sperm cryopreservation and in vitro fertilization techniques for the African turquoise killifish Nothobranchius furzeri

Sperm cryopreservation and in vitro fertilization techniques for the African turquoise killifish Nothobranchius furzeri

Sperm cryopreservation and in vitro fertilization techniques for the African turquoise killifish Nothobranchius furzeri

Direct allele introgression into pure chicken breeds using Sire Dam Surrogate (SDS) mating

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