Preserving Biological Material by Plastination

Bickley, Harmon C.; Townsend, Frank M.

doi:10.1111/j.2151-6952.1984.tb01684.x

Cited by 9 publications

(5 citation statements)

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“…Several ileal sections containing lymphoid tissue were impregnated with curable polymers using the technique of plastination modified from original work by von Hagens (1979)(1980)(1981)(1982)(1983)(1984). Plastination preserves specimens in a form that maintains the appearance of the original tissue (Bickley and Townsend 1984;Boyes 1992).…”

Section: Methodsmentioning

confidence: 99%

Lymphoid tissues of the ileum in young horses: distribution, structure, and epithelium

Lowden

Heath

1995

Anat Embryol

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Lymphoid tissues in the ileum of young horses form raised plaques that are macroscopically visible from the mucosal surface. These are termed "ileal lymphoid patches". These patches are variable in size, shape and position within the ileal wall, occasionally lying along the site of mesenteric attachment. Within lymphoid patches, follicles exist in three different morphological forms: follicle/dome structures, proprial follicles, and lymphoglandular complexes (LGCs). In follicle/dome structures, the majority of the follicle lies in the submucosa and merges with a dome in the lamina propria through a gap in the muscularis mucosae. In proprial follicles, the majority, or all, of the follicle is found in the lamina propria, and in LGCs, the follicles lie in the submucosa and communicate with the intestinal lumen via a central invagination of epithelium that extends vertically through a gap in the muscularis mucosae. Follicle-associated epithelium covers the follicle/dome structures and proprial follicles. It consists of enterocytes, cells morphologically resembling M cells, intraepithelial lymphocytes, goblet cells, and amine-precursor uptake and decarboxylation (APUD) cells. The epithelium of LGCs is mainly populated by immature enterocytes, intraepithelial lymphocytes and goblet cells. Cells with coarse, long microvilli are also present. Information regarding the presence of LGCs in the small intestine is scant, but LGCs have been well described in the large intestine of many species. Further investigation will be required to determine if factors exist that are common to both the ileum of the horse and the large intestine of other species to influence the development of LGCs at these specific sites.

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Section: Methodsmentioning

confidence: 99%

Lymphoid tissues of the ileum in young horses: distribution, structure, and epithelium

Lowden

Heath

1995

Anat Embryol

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“…T h e wall of the left hemithorax of the body was removed, and the anterior approach to the thoracolumbar junction of the vertebral column (6) dissected in layers, together with extensive exposure of the diaphragm in the region of the tenth rib. T h e dissection was then bleached, treated with alcohols to remove the glycerol, and plastinated by means of the S10 technique (7,8); i.e., dehydrated with acetone at -25"C, impregnated under vacuum, still at -25"C, with Biodur@ S10 and S3; and finally, gas-hardened at room temperature with BioduP S6. For further details the reader is referred to the references 4, 7, and 8.…”

Section: Therapeutic Considerationsmentioning

confidence: 99%

Use of plastinated specimens in surgical education and clinical practice

Fasel

1988

Clinical Anatomy

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This paper describes the usefulness of the process of plastination, a new technique of tissue preservation, for demonstrating both diagnostic and therapeutic aspects of advanced surgical anatomy. In contrast to existing methods, plastination provides dry, firm, odorless, and manipulable specimens that are esthetically acceptable everywhere and thus allows anatomy to be both literally and metaphorically carried into the clinic. The author regards this process as an important factor in bridging the gap between anatomy and clinical practice.

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“…In relation to epoxy sheet plastination, can be thin slices (2 to 3 mm) or ultrathin (<2 mm), and can be used for any corporal region with the great advantage that it produces minimal tissue shrinkage compare to polyester sheet plastination (Bickley & Townsend, 1984;Sora et al, 2002;Sora & Matusz, 2010;Ottone et al, 2017). This epoxy resin advantage allows analize morphometric parameters of different structures and anatomical regions, applicable to morphological research (Eckel et al, 1993;Sora et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Epoxy Sheet Plastination on a Rabbit Head - New Faster Protocol with Biodur® E12/E1

et al. 2018

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VARGAS, C. A.; VEUTHEY, C.; DEL SOL, M. & FUENTES, F. Epoxy sheet plastination on a rabbit headnew faster protocol with biodur® E12/E1. Int. J. Morphol., 36(2):441-446, 2018. SUMMARY:Plastination is an anatomical technique of cadaveric preservation that allows the preservation of anatomical pieces indefinitely, in dry and odorless form. It was created in 1978 by Gunther von Hagens, in Heidelberg, Germany. In particular, the sheet plastination technique, with epoxy resin, allows the generation of thin sections of various anatomical regions, allowing an accurate visualization of anatomical structures of difficult access through dissection or cadaveric exploration. The aim of this work was to present a new sheet plastination protocol with Biodur® E12/E1, which is faster in its implementation, applied, for the first time, in a rabbit head.

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Preserving Biological Material by Plastination

Cited by 9 publications

References 0 publications

Lymphoid tissues of the ileum in young horses: distribution, structure, and epithelium

Lymphoid tissues of the ileum in young horses: distribution, structure, and epithelium

Use of plastinated specimens in surgical education and clinical practice

Epoxy Sheet Plastination on a Rabbit Head - New Faster Protocol with Biodur® E12/E1

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