Preserving genetic variance resources in commercial and non-commercial populations

Buis, Roger; Oldenbroek, J.K.; Werf, J.H.J. van der

doi:10.18174/njas.v42i1.611

Cited by 4 publications

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“…On the other hand, the evaluation of genetic contributions from ancestors to descendants is needed, as well as the development of replacement strategies that guarantee a minimum DF. Buis et al (1994) reported the establishment in the Netherlands of a genetic bank for commercial and non-commercial cattle breeds. The genetic bank is a result of the combined action of AI organizations and the government.…”

Section: Discussionmentioning

confidence: 99%

“…21 respectively, indicating that a genetic bottleneck may occur at any period of the Bank. Genetic drift may produce arbitrary changes in genetic frequencies through generations, increasing the probability of gene fixation and decreasing genetic variability (Buis et al 1994). The ratio between the effective number of founder genomes and effective number of founders Ng/fe was 0 .…”

Section: Discussionmentioning

confidence: 99%

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Genetic variability of a Criollo cattle population under conservation

Melucci¹,

Díaz

Mezzadra³

2005

J. Agric. Sci.

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The active germplasm Bank of the Integrated Unit (Facultad de Ciencias Agrarias, Unversidad Nacional de Mar del Plata and the Experimental Station of INTA) at Balcarce, keeps both in vivo (live herd) and in vitro Criollo Cattle (samples of semen and embryos cryogenically), with the aim of preserving genetic variability for potential future use. Parameters based on probable origin of genes, on population size and on the estimation of components of (co)variances for birth and weaning weights were estimated to evaluate the genetic variability of the material contained in the Bank. The information available came from 667 individuals, of which 84 had unknown parents, and 142 half-founders (only one parent known), having a mean number of births per year of 24 calves from four different sires. AI was performed during the mating season almost every year. The evolution of effective number of founders (fe), effective number of ancestors (fa), effective number of founder genomes (Ng) and the ratio fe/fa over different periods from the creation of the Bank, were analysed. Annual increase of inbreeding coefficient (F) was 0·2% and the effective population size (Ne) was estimated as 23, 14 and 25 individuals for the periods defined by years 1978–82, 1983–87 and 1993–97, respectively. The ratio fe/fa was higher in the years where the ratio number of sires/number of dams was lower, indicating that the impact of the effective number of sires is more important than the effective number of females in generating genetic bottlenecks. When the reference population was taken for the period 1989–97, 16·45 out of the 85·63 founders of the population made contributions not explained by other ancestors, but if possible losses of genomes due to genetic drift are considered, the number of genomes still present was reduced to 9·09 indicating a possible narrow genetic bottleneck in the last period. The Ng/fe ratio was 0·11, 0·10 and 0·11 for the reference populations defined by years 1989–92, 1993–97 and 1989–97, respectively. The main objective in germplasm conservation programmes should be the maintenance of genetic variability. Thus, matings in the population under conservation should be designed in order to mate those animals that maximize the effective number of genome founders (Ng) to avoid losses due to genetic drift.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genetic variability of a Criollo cattle population under conservation

Melucci¹,

Díaz

Mezzadra³

2005

J. Agric. Sci.

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“…This trend causes genetic diversity in cattle to decline very rapidly (Hall and Bradley 1995;Hammond and Leitch 1995). In fact, genetic diversity is the basis of cross-breeding for livestock (Buis et al 1994) because this information can be used as a starting point to increase the quantity and quality of species through artificial selection and becomes the initial knowledge B I O D I V E R S I T A S 20 (3): 783-788, March 2019 784 needed in conservation of genetic resources (Kidd et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique

et al. 2019

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Abstract. Sutarno, Zahrah S, Astirin OP, Herawati E, Setyawan AD. 2019. Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique. Biodiversitas 20: 783-788. Genetic diversity is the basis of livestock breeding because it can be used as an initial improvement in livestock quality through artificial selection. This study aims to determine polymorphism in ND-5 fragment of mitochondrial DNA in Ongole Grade, Aceh, and Sumbawa cattle and their genetic diversity. The total DNA from the blood of the local cattle was extracted using the Wizard genomic DNA purification system from Promega and amplified using the PCR technique. The PCR product was then digested with HindIII enzyme using the RFLP technique to detect polymorphism. The genetic diversity of the Ongole Grade, Aceh, and Sumbawa cattle was analyzed using the formula from Nei and its genetic relationship was evaluated with the 2.02i NTSYSpc version program. Our findings showed there were polymorphisms in the ND-5 fragment of mitochondrial DNA. Digestion with HindIII restriction enzyme produces two types of haplotypes. Haplotype B is a 453 bp-sized DNA fragment that is not truncated by the HindIII enzyme, and haplotype A is a DNA fragment cut by HindIII enzyme into two with fragments of 336 bp and 117 bp. Polymorphism was found in Ongole Grade cattle, but not in Sumbawa and Aceh cattle. Haplotypes diversity in ND-5 fragments of mitochondrial DNA of Ongole Grade was 0.6250 while Sumbawa and Aceh cattle displayed no diversity of haplotypes. The genetic relationship shows that Sumbawa cattle belonged to the same cluster with Ongole Grade but separated from Aceh cattle.

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“…Genetic diversity is regarded as the basis of crossbreeding for livestock (Buis et al 1994). Information on the genetic diversity may serve as a starting point for artificial selection in an attempt to increase the quality and quantity of breed.…”

Section: Introductionmentioning

confidence: 99%

Interspecies and intraspecies genetic diversity of Ongole Grade cattle and Madura cattle based on microsatellite DNA markers

Sutarno

Kurnianingrum²,

Herawati

et al. 2018

Biodiversitas

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Sutarno, Kurnianingrum N, Herawati E, Setyawan AD. 2018. Interspecies and intraspecies genetic diversity of Ongole Gradecattle and Madura cattle based on microsatellite DNA markers. Biodiversitas 19: 1593-1600. DNA microsatellite has been extensivelyemployed for estimating the degree of kinship between genotypes and improving the quality of cattle products. Microsatellite markersare short-patterned DNA sequences and repeated tandem (sequentially) with 2-5 nucleotide units scattering the entire genome. Thepurpose of this study was to investigate the genetic characteristics of inter and intraspecies of Ongole Grade cattle and Madura cattleusing microsatellite DNA markers. Blood samples from 20 individuals of each species were extracted by the method referring to WizardGenomic DNA Purification Kit (Promega, USA) and PCR amplification was performed using 5 microsatellite loci, i.e., BM1824,ETH225, INRA005, MM12, and TGLA227. Results of the genetic characteristics of both species were calculated using the POPGENEprogram version 1.31. The data suggest that there is a genetic diversity of inter and intraspecies of Ongole Grade cattle and Maduracattle. The average value of Shannon's Information Index (I) at all microsatellite loci for Ongole Grade cattle was 0.76 and for Maduracattle was 1.12. Meanwhile, the average interspecies I value was 1.03. The mean intraspecies Polymorphic Information Content (PIC) ofOngole Grade and Madura cattle was 0.43, and 0.63, respectively, and the mean interspecies PIC value was 0.57. The data altogethersuggest that all loci meet the standards as being informative markers in the assessment of genetic population because it has a PIC value>0.5 especially for intraspecies of Madura cattle.

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Preserving genetic variance resources in commercial and non-commercial populations

Cited by 4 publications

References 0 publications

Genetic variability of a Criollo cattle population under conservation

Genetic variability of a Criollo cattle population under conservation

Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique

Interspecies and intraspecies genetic diversity of Ongole Grade cattle and Madura cattle based on microsatellite DNA markers

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