Preserving tardigrades under pressure

Seki, Kunihiro; Toyoshima, Manabu

doi:10.1038/27576

Cited by 130 publications

(69 citation statements)

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“…To survive drought, which occurs frequently in the habitat of moss-dwelling tardigrades, they enter a so-called tun state (Baumann 1922) and show strong resistance to physical extremes, including high and low temperatures (Ramløv and Westh 1992;Sømme 1996;Ramløv and Westh 2001;Hengherr et al 2009), high pressure (Seki and Toyoshima 1998), vacuum and ionising irradiation (Horikawa et al 2006;Jönsson et al 2008). In the anhydrobiotic state, their metabolism is barely measurable (Pigoń and Węglarska 1955).…”

Section: Introductionmentioning

confidence: 99%

Stress response in tardigrades: differential gene expression of molecular chaperones

Reuner

Hengherr

Mali

et al. 2010

Cell Stress and Chaperones

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Semi-terrestrial tardigrades exhibit a remarkable tolerance to desiccation by entering a state called anhydrobiosis. In this state, they show a strong resistance against several kinds of physical extremes. Because of the probable importance of stress proteins during the phases of dehydration and rehydration, the relative abundance of transcripts coding for two α-crystallin heat-shock proteins (MtsHsp17.2 and Mt-sHsp19.5), as well for the heat-shock proteins Mt-sHsp10, Mt-Hsp60, Mt-Hsp70 and Mt-Hsp90, were analysed in active and anhydrobiotic tardigrades of the species Milnesium tardigradum. They were also analysed in the transitional stage (I) of dehydration, the transitional stage (II) of rehydration and in heat-shocked specimens. A variable pattern of expression was detected, with most candidates being downregulated. Gene transcripts of one Mt-hsp70 isoform in the transitional stage I and Mt-hsp90 in the anhydrobiotic stage were significantly upregulated. A high gene expression (778.6-fold) was found for the small α-crystallin heat-shock protein gene Mt-sHsp17.2 after heat shock. We discuss the limited role of the stress-gene expression in the transitional stages between the active and anhydrobiotic tardigrades and other mechanisms which allow tardigrades to survive desiccation.

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Section: Introductionmentioning

confidence: 99%

Stress response in tardigrades: differential gene expression of molecular chaperones

Reuner

Hengherr

Mali

et al. 2010

Cell Stress and Chaperones

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“…This physiolog-RESULTS ical mechanism of preserving and resuscitating tardigrades was applied to mammalian organ preservation The isolated rat heart, which was resuscitated after being preserved for 24 h in a 2 ATA high-pressure (12). A tardigrade is a multicellular organism that is composed of about 40,000 cells and also has nerve cells.…”

Section: Methodsmentioning

confidence: 99%

“…Seki et al blood cells and other microorganisms. As a result, it is necessary to establish new methods for long-term organ showed that, under such conditions, tardigrades can regenerate even under ultrahigh pressure (12). preservation (1).…”

Section: Introductionmentioning

confidence: 99%

Study on the Preservation with CO (PCO = 200–2,000 hPa), Resuscitation, and Heterotopic Transplantation of an Isolated Rat Heart

2009

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In this experiment, CO was used as a gas mixture in a reversible relationship with O 2 . CO was added in a gas form mixed with O 2 . An isolated donor rat heart was obtained, exposed to a gas mixture such as PO 2 = 1,800 hPa and PCO = 200 hPa, and PO 2 = 1,000 hPa and PCO = 1,000 hPa in a 2 ATA high-pressure chamber and preserved in a refrigerator at 4°C. This report demonstrates that significant reproducibility has been verified. The heart was removed from the refrigerator 24 h later and heterotopic heart transplantation was performed in the right neck of a recipient rat and the pulsating of the transplanted heart was detected by an electrocardiogram.

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“…They have been shown to resist extremes of heat (Doyère 1842;Rahm 1921) and cold (Becquerel 1950;Hengherr et al 2009), radiation (May et al 1964), high pressure, and vacuum (Seki and Toyoshima 1998)-indeed, they are the only animals so far to have been deliberately exposed to the vacuum and radiation of space, which they survived (Jönsson et al 2008).…”

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confidence: 99%

Tardigrades of Fish Creek Provincial Park, Alberta, Canada: A Preliminary Survey

Grothman

2011

Can Field Nat

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Tardigrades are cosmopolitan animals with a patchy distribution record, largely due to the limited collection history for this phylum rather than actual rarity. Most Canadian records are from near coastal areas, with a few notable exceptions, most recently one study of tardigrades in central Alberta. The present study reports the occurrence of 12 species or species groups in Fish Creek Provincial Park in southern Alberta. Eight of these taxa are new records for Alberta, and two (Diphascon (Diphascon) granifer and Echiniscus granulatus) are new records for Canada.

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Preserving tardigrades under pressure

Cited by 130 publications

References 0 publications

Stress response in tardigrades: differential gene expression of molecular chaperones

Stress response in tardigrades: differential gene expression of molecular chaperones

Study on the Preservation with CO (PCO = 200–2,000 hPa), Resuscitation, and Heterotopic Transplantation of an Isolated Rat Heart

Tardigrades of Fish Creek Provincial Park, Alberta, Canada: A Preliminary Survey

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