Preserving the activity of enzymes under harsh oxidizing conditions: sol–gel entrapped alkaline phosphatase exposed to bromine

Frenkel-Mullerad, Hagit; Ben-Knaz, Racheli; Avnir, David

doi:10.1007/s10971-013-3226-z

Cited by 10 publications

(9 citation statements)

References 23 publications

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“…Our current knowledge of sol–gel-derived biomaterials is largely based on the intensive studies of silica: with this oxide, methodologies of entrapment were developed, stability studies were carried out, and enzymatic activities were analyzed . On the advantages side, silica sol–gel materials not only preserve the activity of the bioactive dopants, but also provide exceptional stability to a variety of proteins and bioactive molecules against various deterioration processes. − And yet, an elementary, key limitation of silica that has been a major obstacle towards development of medical applications is the fact that this oxide is not approved by medical regulators for injection. However, this limitation on sol–gel materials can be addressed if the scope is broadened beyond silica.…”

Section: Introductionmentioning

confidence: 99%

Entrapment of Enzymes within Sol–Gel-Derived Magnetite

et al. 2016

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Magnetically controlled enzymatic composites have received much attention for both therapeutic and industrial applications. Until now, such materials have been composed of at least four components: the enzyme, magnetic nanoparticles, their stabilizing components, and an organic or inorganic (or hybrid) matrix as a carrier. However, such compositions affect the magnetic response and the enzymatic activity, and also pose obstacles for intravenous administration, because of regulatory restrictions. Here, we present a methodology for the creation of magnetic bioactive nanocomposites composed of only two biocompatible components: an enzyme and magnetite nanoparticles. A series of magnetic biocomposites with a full set of therapeutical and industrial proteins (carbonic anhydrase, ovalbumin, horseradish peroxidase, acid phosphatase, proteinase, and xylanase) were successfully created by the direct entrapment of the proteins within a sol–gel magnetite matrix specially developed for these aims. The activity of the entrapped enzymes was studied at different temperatures and concentrations, and it was found that they showed remarkable thermal stabilization induced by the ferria matrix. For instance, entrapped carbonic anhydrase catalyzed the decomposition of p-nitrophenylacetate at a temperature of 90 °C, while free enzyme completely loses activity and denaturates already at 70 °C. Magnetic characterization of the obtained biomaterials is provided.

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Section: Introductionmentioning

confidence: 99%

Entrapment of Enzymes within Sol–Gel-Derived Magnetite

et al. 2016

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“…In the last years, sol-gel processing emerged as a versatile methodology for coating surfaces with multifunctional purposes [12][13][14][15][16] that include several bio-applications such as enzyme 17,18 , living cells 19 and fungi entrapment for biocatalysis 20 . Although the capacity of the sol-gel process to produce versatile coatings able to enhance the interaction between biomaterials and living cells is well known, only few efforts have been made to exploit the use of this procedure to obtain antibacterial coatings.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Self-sterilizing ormosils surfaces based on photo-synzthesized silver nanoparticles

Gonçalves

Miñán

Benítez

et al. 2018

Colloids and Surfaces B: Biointerfaces

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Medical device-related infections represent a major healthcare complication, resulting in potential risks for the patient. Antimicrobial materials comprise an attractive strategy against bacterial colonization and biofilm proliferation. However, in most cases these materials are only bacteriostatic or bactericidal, and consequently they must be used in combination with other antimicrobials in order to reach the eradication condition (no viable microorganisms). In this study, a straightforward and robust antibacterial coating based on Phosphotungstate Ormosil doped with core-shell (SiO@TiO) was developed using sol-gel process, chemical tempering, and Ag nanoparticle photoassisted synthesis (POrs-CS-Ag). The coating was characterized by X-ray Fluorescence Spectroscopy (XRF), Field Emission Scanning Electron Microscopy (FE-SEM), Atomic Force Microscopy (AFM) and X-ray Photoelectron Microscopy (XPS). The silver free coating displays low antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa, in opposition to the silver loaded ones, which are able to completely eradicate these strains. Moreover, the antimicrobial activity of these substrates remains high until three reutilization cycles, which make them a promising strategy to develop self-sterilizing materials, such as POrs-CS-Ag-impregnated fabric, POrs-CS-Ag coated indwelling metals and polymers, among other materials.

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“…Moreover, large variety of reagents can be entrapped in the matrices produced using the sol‐gel process [49,67,68,78–85] . Previous studies by Avnir et al., [86–90] Shamir et al [68] . and Aharon et al [49] .…”

Section: Introductionmentioning

confidence: 99%

Heterogeneous Electrocatalytic Oxygen Evolution Reaction by a Sol‐Gel Electrode with Entrapped Na₃[Ru₂(μ‐CO₃)₄]: The Effect of NaHCO₃

Aharon,

Patra,

Meyerstein

et al. 2023

ChemPhysChem

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The Na3[Ru2(µ‐CO3)4] complex is acting as a water oxidation catalyst in a homogeneous system. Due to the significance of heterogeneous systems and the effect of bicarbonate on the kinetic, we studied the bicarbonate effect on the heterogeneous electrocatalyst by entrapping the Na3[Ru2(µ‐CO3)4] complex in a sol‐gel matrix. We have developed two types of sol‐gel electrodes, which differ by the precursor, and are demonstrating their stability over a minimum of 200 electrochemical cycles. The pH increases affected the currents and kcat for both types of electrodes, and their hydrophobicity, which was obtained from the precursor type, influenced the electrocatalytic process rate. The results indicate that NaHCO3 has an important role in the catalytic activity of the presented heterogeneous systems; without NaHCO3, the diffusing species is probably OH‐, which undergoes diffusion via the Grotthuss mechanism. To the best of our knowledge, this is the first study to present a simple and fast one‐step entrapment process for the Na3[Ru2(µ‐CO3)4] complex by the sol‐gel method under standard laboratory conditions. The results contribute to optimizing the WSP, ultimately helping expand the usage of hydrogen as a green and more readily available energy source.

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Preserving the activity of enzymes under harsh oxidizing conditions: sol–gel entrapped alkaline phosphatase exposed to bromine

Cited by 10 publications

References 23 publications

Entrapment of Enzymes within Sol–Gel-Derived Magnetite

Entrapment of Enzymes within Sol–Gel-Derived Magnetite

Self-sterilizing ormosils surfaces based on photo-synzthesized silver nanoparticles

Heterogeneous Electrocatalytic Oxygen Evolution Reaction by a Sol‐Gel Electrode with Entrapped Na₃[Ru₂(μ‐CO₃)₄]: The Effect of NaHCO₃

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Preserving the activity of enzymes under harsh oxidizing conditions: sol–gel entrapped alkaline phosphatase exposed to bromine

Cited by 10 publications

References 23 publications

Entrapment of Enzymes within Sol–Gel-Derived Magnetite

Entrapment of Enzymes within Sol–Gel-Derived Magnetite

Self-sterilizing ormosils surfaces based on photo-synzthesized silver nanoparticles

Heterogeneous Electrocatalytic Oxygen Evolution Reaction by a Sol‐Gel Electrode with Entrapped Na3[Ru2(μ‐CO3)4]: The Effect of NaHCO3

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Product

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Heterogeneous Electrocatalytic Oxygen Evolution Reaction by a Sol‐Gel Electrode with Entrapped Na₃[Ru₂(μ‐CO₃)₄]: The Effect of NaHCO₃