Pressure-flow relationship of erythrocyte suspension in perfusion of Nuclepore membrane and red cell deformability.

Uyesaka, Nobuhiro

doi:10.2170/jjphysiol.38.145

Cited by 3 publications

(1 citation statement)

References 16 publications

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“…RBCs were filtered through nickel mesh (4) using the vertical tube method (21) with a minor modification (24). The experiments were carried out at 24°C by circulating isotherm water through the jacket surrounding the vertical tube, whose inner diameter was 4 mm.…”

Section: Methodsmentioning

confidence: 99%

Effect of Low-Osmolality Contrast Media on Red Cell Filterability

Imai¹,

Hiruma²,

Kumazaki³

et al. 1993

Acta Radiol

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The effects of low-osmolality contrast media (CM) on red blood cell (RBC) filterability were investigated using a recently developed nickel mesh filtration method. The conventional hypertonic CM iothalamate, low-osmolality iohexol, and the recently synthesized iomeprol were studied. Among them, the osmolality of iomeprol was the lowest. The impact of CM osmolality, viscosity, and iodine content on the RBC filterability was analyzed. Under equal iodine content or viscosity condition, the filterability order of RBCs suspended in CM was iomeprol > iohexol > > iothalamate, because of the osmolality of CM. Iomeprol caused small echinocytic changes but these had a negligible influence on RBC filterability. In conclusion, the osmotic effect of CM on RBC filterability is more predominant than the other CM effects, and iomeprol is the preferred CM for RBC filterability.

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Section: Methodsmentioning

confidence: 99%

Effect of Low-Osmolality Contrast Media on Red Cell Filterability

Imai¹,

Hiruma²,

Kumazaki³

et al. 1993

Acta Radiol

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Sickle cell rheology is determined by polymer fraction–not cell morphology

et al. 1995

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Sickle cell disease pathophysiology is mediated by acute and chronic impairment of cell flexibility due to the formation of intracellular sickle hemoglobin (Hb S) polymer as cells are partially deoxygenated in the microcirculation. We have recently developed a method to measure the relationship between the formation of intracellular polymerized Hb S and cell filtration. In this study, we have used this method to examine whether sickle cell morphology, independent of Hb S polymer fraction, had an effect on cell rheology. We primarily use sickle trait (AS) and Hb S-beta(+)-thalassemia (S-beta(+)-thal) erythrocytes with low hemoglobin F levels, which have normal membranes and few or no dense cells, to remove these confounding effects. We find that the relationship between filtration and the percentages of each "type" of morphological deformation of AS erythrocytes was different from that of the S-beta(+)-thal erythrocytes. In addition, we find that while the filtration of AS erythrocytes as a function of oxygen saturation was similar, whether measured during deoxygenation or reoxygenation, the relationship between the percentages of each type of deformed erythrocyte and oxygen saturation demonstrated hysteresis during oxygenation-deoxygenation experiments. Transmission electron microscopy, for both elongated and irregularly shaped cells, showed that similarly distorted cells could have very different amounts and alignment of polymer. These results suggests that cell morphology per se is not strongly related to filtration, whereas calculated intracellular Hb S polymer fraction predicts loss of filtration of AS and S-beta(+)-thal erythrocytes well. Measured or calculated polymer fraction values would appear to be a better parameter for the study of sickle cell disease pathophysiology and response to treatment than cell morphology studies.

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