2000
DOI: 10.1161/01.atv.20.1.96
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Pressure-Induced Upregulation of Preproendothelin-1 and Endothelin B Receptor Expression in Rabbit Jugular Vein In Situ

Abstract: Abstract-Upregulation of endothelin-1 (ET-1) synthesis in venous bypass grafts in response to arterial levels of blood pressure may play a major role in graft failure. To investigate this hypothesis, isolated segments of the rabbit jugular vein were perfused at physiological (0 to 5 mm Hg) and nonphysiological (20 mm Hg) levels of intraluminal pressure.As judged by reverse transcription-polymerase chain reaction analysis (mRNA level), neither endothelin-converting enzyme nor endothelin A receptor expression ap… Show more

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Cited by 32 publications
(32 citation statements)
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“…8,9 EF-1 expression was not pressure-sensitive as previously demonstrated. 8,9 Immunohistochemistry Formaldehyde-fixed sections of the carotid artery and jugular vein were embedded in paraffin blocks of which 7 mm thick sections were cut.…”
Section: Rt ± Pcr Analysissupporting
confidence: 69%
See 2 more Smart Citations
“…8,9 EF-1 expression was not pressure-sensitive as previously demonstrated. 8,9 Immunohistochemistry Formaldehyde-fixed sections of the carotid artery and jugular vein were embedded in paraffin blocks of which 7 mm thick sections were cut.…”
Section: Rt ± Pcr Analysissupporting
confidence: 69%
“…8,9 EF-1 expression was not pressure-sensitive as previously demonstrated. 8,9 Immunohistochemistry Formaldehyde-fixed sections of the carotid artery and jugular vein were embedded in paraffin blocks of which 7 mm thick sections were cut. After paraffin removal (boiling of the glass-fixed sections six times for 5 min in 10 mmol/L sodium citrate, pH 6.0), they were inactivated in 10 mmol/L Tris buffer, pH 7.6 containing 3% (v/v) hydrogen peroxide followed by blocking of non-specific binding sites for 20 min at room temperature with 10% (w/v) bovine serum albumin in Hank's balanced salt solution (Life Technologies).…”
Section: Rt ± Pcr Analysissupporting
confidence: 69%
See 1 more Smart Citation
“…ET-1 is not only a powerful co-mitogen, 13 its synthesis by the endothelium also is strongly up-regulated by (blood) pressureinduced distension of the vessel wall. 9 In addition, ET B -R mRNA abundance in the SMC is markedly increased under these conditions.…”
Section: Discussionmentioning
confidence: 92%
“…8,9 ) a new series of experiments was performed both with isolated perfused segments of the carotid artery and jugular vein of the rabbit (Figure 1). In both types of blood vessels prepro-ET-1 expression and that of the ET B -R were up-regulated in response to perfusion at a supra-physiological pressure of 160 and 20 mm Hg, respectively, for 6 h. ET A -R expression and that of endothelin-converting enzyme 1 (ECE-1), on the other hand, appeared to be pressure-insensitive.…”
Section: Pressure-dependent Activation Of the Endothelin Systemmentioning
confidence: 99%