Pressure Overload Creates Right Ventricular Diastolic Dysfunction in a Mouse Model: Assessment by Echocardiography

Egemnazarov, Bakytbek; Schmidt, Albrecht; Crnković, Slaven; Sydykov, Akylbek; Nagy, Bence; Kovàcs, Gabór; Weißmann, Norbert; Olschewski, Horst; Olschewski, Andrea; Kwapiszewska, Grażyna; Marsh, Leigh M.

doi:10.1016/j.echo.2015.02.014

Cited by 32 publications

(44 citation statements)

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“…The stenosis rats exhibited an increased ratio of RV weight to body weight and cross-section cardiomyocyte area compared with SHAM rats, in which trained animals showed a lower cross-section cardiomyocyte area compared to SS group (Figure 3B). Our structural analysis corroborates a well-established finding of myocardial fibrosis associated with RV pressure overload (Baicu et al, 2012; Egemnazarov et al, 2015). Thereby, stenosis resulted in a significant increase in collagen content (Figure 3C).…”

Section: Resultssupporting

confidence: 90%

“…To date, although systolic and diastolic dysfunction has been reported in our PAS model, we did not observe signs of cardiac failure, as shown by the lack of cyanosis and liver or lung congestion. These findings are in line with interpretations from other groups (Johnson et al, 2011; Egemnazarov et al, 2015) and emerged as a possibility to explain because no significant reduction in functional capacity was noticed with PAS (Figure 2C). …”

Section: Discussionsupporting

confidence: 92%

“…As previously described by other authors (Egemnazarov et al, 2015), myocardial remodeling was associated with reactivation of the “fetal gene program,” as shown by the shift from α-MHC to β-MHC expression. Thus, as seen in Figure 4, the β-MHC to α-MHC ratio was shifted upwards in transcriptional and translational level.…”

Section: Discussionsupporting

confidence: 64%

“…The right parasternal short-axis view at the level of the papillary muscles was used for determination of the diastolic and systolic RV area. Measurements of the RV outflow tract were obtained from the parasternal short-axis view at the level of the aortic valve during end-diastole (Egemnazarov et al, 2015). We included only animals with pressure gradients between 25–55 mmHg (Manchini et al, 2014).…”

Section: Methodsmentioning

confidence: 99%

“…The RV fibrosis was evaluated in tissue samples stained with picro-sirius red. The collagen content was assessed at 40× magnification using an Olympus image acquisition system (Egemnazarov et al, 2015). The percentage fibrosis was calculated by dividing the total area of collagen by the total analyzed tissue area multiplied by 100.…”

Section: Methodsmentioning

confidence: 99%

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Exercise Training Attenuates Right Ventricular Remodeling in Rats with Pulmonary Arterial Stenosis

et al. 2016

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Introduction: Pulmonary arterial stenosis (PAS) is a congenital defect that causes outflow tract obstruction of the right ventricle (RV). Currently, negative issues are reported in the PAS management: not all patients may be eligible to surgeries; there is often the need for another surgery during passage to adulthood; patients with mild stenosis may have later cardiac adverse repercussions. Thus, the search for approaches to counteract the long-term PAS effects showed to be a current target. At the study herein, we evaluated the cardioprotective role of exercise training in rats submitted to PAS for 9 weeks.Methods and Results: Exercise resulted in improved physical fitness and systolic RV function. Exercise also blunted concentric cavity changes, diastolic dysfunction, and fibrosis induced by PAS. Exercise additional benefits were also reported in a pro-survival signal, in which there were increased Akt1 activity and normalized myocardial apoptosis. These findings were accompanied by microRNA-1 downregulation and microRNA-21 upregulation. Moreover, exercise was associated with a higher myocardial abundance of the sarcomeric protein α-MHC and proteins that modulate calcium handling—ryanodine receptor and Serca 2, supporting the potential role of exercise in improving myocardial performance.Conclusion: Our results represent the first demonstration that exercise can attenuate the RV remodeling in an experimental PAS. The cardioprotective effects were associated with positive modulation of RV function, survival signaling pathway, apoptosis, and proteins involved in the regulation of myocardial contractility.

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Section: Resultssupporting

confidence: 90%

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 64%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Exercise Training Attenuates Right Ventricular Remodeling in Rats with Pulmonary Arterial Stenosis

et al. 2016

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Mouse Cardiovascular Imaging

Phoon,

Aristizábal,

Farhoud

et al. 2024

Current Protocols

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The mouse is the mammalian model of choice for investigating cardiovascular biology, given our ability to manipulate it by genetic, pharmacologic, mechanical, and environmental means. Imaging is an important approach to phenotyping both function and structure of cardiac and vascular components. This review details commonly used imaging approaches, with a focus on echocardiography and magnetic resonance imaging, with brief overviews of other imaging modalities. In this update, we also emphasize the importance of rigor and reproducibility in imaging approaches, experimental design, and documentation. Finally, we briefly outline emerging imaging approaches but caution that reliability and validity data may be lacking. © 2024 Wiley Periodicals LLC.

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Right‐ventricular dysfunction in HFpEF is linked to altered cardiomyocyte Ca²⁺ homeostasis and myofilament sensitivity

et al. 2021

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Aims Heart failure with preserved ejection fraction (HFpEF) is frequently (30%) associated with right ventricular (RV) dysfunction, which increases morbidity and mortality in these patients. Yet cellular mechanisms of RV remodelling and RV dysfunction in HFpEF are not well understood. Here, we evaluated RV cardiomyocyte function in a rat model of metabolically induced HFpEF. Methods and results Heart failure with preserved ejection fraction-prone animals (ZSF-1 obese) and control rats (Wistar Kyoto) were fed a high-caloric diet for 13 weeks. Haemodynamic characterization by echocardiography and invasive catheterization was performed at 22 and 23 weeks of age, respectively. After sacrifice, organ morphometry, RV histology, isolated RV cardiomyocyte function, and calcium (Ca 2+ ) transients were assessed. ZSF-1 obese rats showed a HFpEF phenotype with left ventricular (LV) hypertrophy, LV diastolic dysfunction (including increased LV end-diastolic pressures and E/e0 ratio), and preserved LV ejection fraction. ZSF-1 obese animals developed RV dilatation (50% increased end-diastolic area) and mildly impaired RV ejection fraction (42%) with evidence of RV hypertrophy. In isolated RV cardiomyocytes from ZSF-1 obese rats, cell shortening amplitude was preserved, but cytosolic Ca 2+ transient amplitude was reduced. In addition, augmentation of cytosolic Ca 2+ release with increased stimulation frequency was lost in ZSF-1 obese rats. Myofilament sensitivity was increased, while contractile kinetics were largely unaffected in intact isolated RV cardiomyocytes from ZSF-1 obese rats. Western blot analysis revealed significantly increased phosphorylation of cardiac myosin-binding protein C (Ser282 cMyBP-C) but no change in phosphorylation of troponin I (Ser23, 24 TnI) in RV myocardium from ZSF-1 obese rats. Conclusions Right ventricular dysfunction in obese ZSF-1 rats with HFpEF is associated with intrinsic RV cardiomyocyte remodelling including reduced cytosolic Ca 2+ amplitudes, loss of frequency-dependent augmentation of Ca 2+ release, and increased myofilament Ca 2+ sensitivity.

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Pressure Overload Creates Right Ventricular Diastolic Dysfunction in a Mouse Model: Assessment by Echocardiography

Cited by 32 publications

References 45 publications

Exercise Training Attenuates Right Ventricular Remodeling in Rats with Pulmonary Arterial Stenosis

Exercise Training Attenuates Right Ventricular Remodeling in Rats with Pulmonary Arterial Stenosis

Mouse Cardiovascular Imaging

Right‐ventricular dysfunction in HFpEF is linked to altered cardiomyocyte Ca²⁺ homeostasis and myofilament sensitivity

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Pressure Overload Creates Right Ventricular Diastolic Dysfunction in a Mouse Model: Assessment by Echocardiography

Cited by 32 publications

References 45 publications

Exercise Training Attenuates Right Ventricular Remodeling in Rats with Pulmonary Arterial Stenosis

Exercise Training Attenuates Right Ventricular Remodeling in Rats with Pulmonary Arterial Stenosis

Mouse Cardiovascular Imaging

Right‐ventricular dysfunction in HFpEF is linked to altered cardiomyocyte Ca2+ homeostasis and myofilament sensitivity

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Right‐ventricular dysfunction in HFpEF is linked to altered cardiomyocyte Ca²⁺ homeostasis and myofilament sensitivity