Pressure simulation of orthodontic force in osteoblasts: a pilot study

Baumert, Uwe; Golan, Ilan; Becker, Bradley D.; Bp, Hrala; Redlich, Meir; Ha, Roos; Palmon, Aaron; Reichenberg, E.; Müßig, D

doi:10.1046/j.1601-6335.2003.00270.x

Cited by 26 publications

(20 citation statements)

References 29 publications

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“…Consider F = 1.18 × 10 −5 × R × RS 2 (Figure 1(a)). Then SCAPs were cultured in the plates and centrifuged at 200 g (corresponded to a speed at 1000 rpm) for 30 min [15], which resembles the clinical orthodontic force, and then returned to the incubator. Control cells were cultured in absence of centrifugal loading.…”

Section: Methodsmentioning

confidence: 99%

Mechanical Stress Stimulates the Osteo/Odontoblastic Differentiation of Human Stem Cells from Apical Papilla via ERK 1/2 and JNK MAPK Pathways

Wang

et al. 2014

BioMed Research International

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Background Information. Stem cells from apical papilla (SCAPs) are a potent candidate for the apexogenesis/apexification due to their multiple differentiation capacity. During the orthodontic treatment of developing teeth, SCAPs in vivo are usually subjected to the cyclic stress induced by compression forces. However, it remains unclear whether mechanical stress can affect the proliferation and differentiation of human SCAPs. Results. Human SCAPs were isolated and stimulated by 200 g mechanical stimuli for 30 min and their proliferation and differentiation capacity were evaluated in vitro at different time points. MTT and FCM results demonstrated that cell proliferation was enhanced, while TEM findings showed the morphological and ultrastructural changes in stress-treated SCAPs. ALP activity and mineralization capacity of stress-treated SCAPs were upregulated . In the meantime, higher odontogenic and osteogenic differentiation were found in stress-treated SCAPs by real-time RT-PCR and Western blot, as indicated by the expression of related markers at both mRNA and protein levels. Moreover, the protein expressions of pJNK and pERK MAPK pathways were upregulated. Conclusion. Together, these findings suggest that mechanical stress is an important factor affecting the proliferation and differentiation of SCAPs via the activation of ERK and JNK signaling pathway.

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Section: Methodsmentioning

confidence: 99%

Mechanical Stress Stimulates the Osteo/Odontoblastic Differentiation of Human Stem Cells from Apical Papilla via ERK 1/2 and JNK MAPK Pathways

Wang

et al. 2014

BioMed Research International

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“…where P = kg pressure per cm 2 of cells, m = mass of medium (0.002 kg), r = radius (0.09 m), rpm = revolution /min (600 or 4,800) and A = area of contact between medium and cells (9.62 cm 2 ) (20)(21)(22)(23). The cells on the dishes were then cultured continuously up to 7 days.…”

Section: Procedures Of Treating Cells With Centrifugal Forcementioning

confidence: 99%

Mechanical force promotes proliferation and early differentiation of bone marrow derived osteoblast-like cells in vitro

et al. 2009

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Abstract:The aim of this study was to examine the effect of centrifugal force on rat bone marrow osteoblast-like cells (RBM) in vitro. RBM were seeded and cultured for 2 days. Centrifugal forces were added to the cells in the culture dishes using a centrifuge for 20 min either at 600 rpm or 4,800 rpm. The cells without centrifugation were used as control. The expression of HSP27 mRNA of both experimental groups after addition of centrifugal force for 3 hrs was significantly up-regulated compared with the control group. In the 4,800 rpm groups, the cells showed contracted shapes compared to the controls and the 600 rpm groups after treatment with centrifugal force. The maximum height of each cell in the control group was 2.00 ± 0.33 μm. The maximum height of each cell in the 600 rpm group was 1.50 ± 0.23 μm, and in the 4,800 rpm group it was 1.13 ± 0.23 μm. The proliferation ratio in the 4,800 rpm group at day 3 was significantly higher than in the other group. The expression of type I collagen and alkaline phosphatase (ALP) mRNAs in both experimental groups increased at earlier time periods compared to the control groups. ALP activity revealed no significant difference between the experimental groups and the control group. A centrifugal force of 4,800 rpm might promote the proliferation and early differentiation of rat bone marrow cells.

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“…In contrast, the former study by Chang et al did not show an increase in proteins that contribute to ECM formation. Similarly, mechanical stimuli have been shown to promote cellular responses in the form of centrifugal force induced RUNX2 expression in osteoblasts [Baumert et al, 2004], mechanical strain of SaOS-2 cells in vitro affects expression of collagen genes types I and III [Liu et al, 2005], and changes in the mechanical environment such as simulated microgravity or substrate deformation have been shown to affect cellular behavior [Granet et al, 2001[Granet et al, , 2002.…”

Section: Introductionmentioning

confidence: 99%

The effects of pulsed electromagnetic fields on the cellular activity of SaOS‐2 cells

Martino¹,

Belchenko²,

Ferguson³

et al. 2007

Bioelectromagnetics

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Although pulsed electromagnetic fields (PEMFs) have been used for treatments of nonunion bone fracture healing for more than three decades, the underlying cellular mechanism of bone formation promoted by PEMFs is still unclear. It has been observed that a series of parameters such as pulse shape and frequency should be carefully controlled to achieve effective treatments. In this article, the effects of PEMFs with repetitive pulse burst waveform on the cellular activity of SaOS-2 osteoblast-like cells were investigated. In particular, cell proliferation and mineralization due to the imposed PEMFs were assessed through direct cell counts, the MTT assay, tissue nonspecific alkaline phosphatase (ALP) and Alizarin Red S (ARS) staining. PEMF stimulation with repetitive pulse burst waveform did not affect metabolic activity and cell number. However, the ALP activity of SaOS-2 cells and mineral nodule formation increased significantly after PEMF stimulation. These observations suggest that repetitive pulse burst PEMF does not affect cellular metabolism; however, it may play a role in the enhancement of SaOS-2 cell mineralization. We are currently investigating cellular responses under different PEMF waveforms and Western blots for protein expression of bone mineralization specific proteins.

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Pressure simulation of orthodontic force in osteoblasts: a pilot study

Cited by 26 publications

References 29 publications

Mechanical Stress Stimulates the Osteo/Odontoblastic Differentiation of Human Stem Cells from Apical Papilla via ERK 1/2 and JNK MAPK Pathways

Mechanical Stress Stimulates the Osteo/Odontoblastic Differentiation of Human Stem Cells from Apical Papilla via ERK 1/2 and JNK MAPK Pathways

Mechanical force promotes proliferation and early differentiation of bone marrow derived osteoblast-like cells in vitro

The effects of pulsed electromagnetic fields on the cellular activity of SaOS‐2 cells

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