Pressure‐temperature studies on the hyaline membrane of sea urchin eggs

Asterita, Harvey; Marsland, Douglas

doi:10.1002/jcp.1030640207

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…If, on the other hand, during gel-to-sol transformation, one or more of these intercellular factors are broken down, more marked changes in cell shape would be expected, and this, in fact, is just what was observed . As summarized in Table I, hydrostatic pressure reduces the lateral infoldings and solates the hyaline layer (Asterita and Marsland, 1964) ; cells tend to spherulate. Prolonged treatment with calciumfree seawater in which all the extracellular devices disappear causes almost complete spherulation.…”

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confidence: 99%

MICROTUBULES IN THE FORMATION AND DEVELOPMENT OF THE PRIMARY MESENCHYME IN ARBACIA PUNCTULATA

Tilney

Gibbins

1969

The Journal of Cell Biology

179

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To experimentally test the suggestion made in the preceding paper that the microtubules are involved in cell shape development during the formation and differentiation of the primary mesenchyme, we applied to the embryos two types of agents which affect cytoplasmic microtubules : (a) colchicine and hydrostatic pressure, which cause the microtubules to disassemble, and (b) D20, which tends to stabilize them . When the first type of agent is applied to sea urchin gastrulae, the development of the primary mesenchyme ceases, the microtubules disappear, and the cells tend to spherulate . With D2O development also ceases, but the tubules appear "frozen," and the cell asymmetries persist unaltered . These agents appear to block development by primarily interfering with the sequential disassembly and/or reassembly of microtubules into new patterns . The microtubules, therefore, appear to be influential in the development of cell form . On the other hand through a careful analysis of the action of these agents and others on both intra-and extracellular factors, we concluded that the microtubules do rather little for the maintenance of cell shape in differentiated tissues .

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confidence: 99%

MICROTUBULES IN THE FORMATION AND DEVELOPMENT OF THE PRIMARY MESENCHYME IN ARBACIA PUNCTULATA

Tilney

Gibbins

1969

The Journal of Cell Biology

179

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Pressure‐temperature effects on the form‐stability and movements of Euglena gracilis var. Z.

Byrne

Marsland

1965

J. Cell. Comp. Physiol.

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The phytoflagellate, Euglena gracilis var Z , maintained in a chemically defined culture medium, was subjected to various high hydrostatic pressures (10,000-15,000 psi) a t various temperatures (15" 20" and 25°C). The effects on form stability, locomotion, euglenoid movement and flagellatory activity were observed at various times after the application of pressure.The pressure-temperature relations of form stability clearly show that the supporting gel structures of the cell, presumably the peripheral gelated cytoplasm and the pellicle, may be identified as Type I1 systems i n the Freundlich classification, since they undergo solational weakening with increasing pressure and with decreasing temperature. The gelational state of these structures appears to be unusually high, however, since at each given temperature, a n usually high intensity of pressure is required to produce a n observable loss of stability, i.e. a rounding up of the normally elongate cells.Flagellatory movement continued even a t the highest pressure, but locomotion was abolished i n all rounded and partly rounded individuals. Euglenoid movement ceased a t relatively lower pressure (about 10,000 psi).The rounding of the euglena, even a t maximum pressures, was not accompanied by any cytolysis. In this respect, euglena resembles amoeba and other sarcodinans, rather than paramecium and other ciliates. Euglena exhibited a rapid transient post-pressure contraction, similar to that which has been described in several other protistan forms. No irreversible changes were observed. Normal form and activity reappeared in almost all specimens within about five minutes, subsequent to decompression.

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High-Pressure Studies in Cell Biology

Zimmerman

1971

International Review of Cytology

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Pressure‐temperature studies on the hyaline membrane of sea urchin eggs

Cited by 3 publications

References 24 publications

MICROTUBULES IN THE FORMATION AND DEVELOPMENT OF THE PRIMARY MESENCHYME IN ARBACIA PUNCTULATA

MICROTUBULES IN THE FORMATION AND DEVELOPMENT OF THE PRIMARY MESENCHYME IN ARBACIA PUNCTULATA

Pressure‐temperature effects on the form‐stability and movements of Euglena gracilis var. Z.

High-Pressure Studies in Cell Biology

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