Pressurized liquid extraction as an eco-friendly approach to recover anthocyanin from black rice bran

Leonarski, Eduardo; Kuasnei, Mayara; Moraes, Paulo Alexandre Durant; Cesca, Karina; Oliveira, Débora de; Zielinski, Acácio Antônio Ferreira

doi:10.1016/j.ifset.2023.103372

Cited by 10 publications

(9 citation statements)

References 58 publications

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“…Black rice bran (BRB) was supplied by Ruzene (Pindamonhangaba, Brazil) and prepared according Leonarski et al [5].…”

Section: Sample Preparationmentioning

confidence: 99%

“…By rotary evaporation, the recovered solution was concentrated and lyophilized. The total monomeric anthocyanin (TMA) analysis was performed according to the differential pH methodology [16], as described by Leonarski et al [5]. The TMA concentrations were calculated using Equations ( 3) and (4): 𝐴 .…”

Section: Partial Purification Of Anthocyanin-rich Extractsmentioning

confidence: 99%

“…The individual anthocyanins were determined by HPLC-MS and quantified by HPLC-PDA, as described by Leonarski et al [5]. The lyophilized extracts were resuspended in formic acid 0.1% and filtered through a 0.22 µm filter.…”

Section: Determining the Individual Anthocyanins By Hplc-msmentioning

confidence: 99%

“…Black rice bran is a rich anthocyanin source, which has been reported to have antidiabetic effects by inhibiting the activities of α-amylase and α-glucosidase (reducing the risk of type 2 diabetes) [6,7]. Furthermore, it was also found that black rice bran extract has a cytoprotective effect on H 2 O 2 -induced oxidative stress in L292 cells, indicating that these extracts may have protective effects against oxidative reactions [5]. Therefore, the recovery of these extracts which are rich in anthocyanins may be interesting for evaluating their biological potential.…”

Section: Introductionmentioning

confidence: 99%

“…Black rice processing produces about 10% rice bran, 14% broken rice, and 20% rice husk [1][2][3], which also has high functional value mainly due to its high anthocyanin content [4,5]. Black rice bran is a rich anthocyanin source, which has been reported to have antidiabetic effects by inhibiting the activities of α-amylase and α-glucosidase (reducing the risk of type 2 diabetes) [6,7].…”

Section: Introductionmentioning

confidence: 99%

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The Potential of Crude and Partially Purified Black Rice Bran Extracts Obtained by Ultrasound-Assisted Extraction: Anti-Glycemic, Cytotoxicity, Cytoprotective, and Antitumoral Effects

Leonarski,

Kuasnei,

Santos

et al. 2024

Foods

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Recovering anthocyanins from black rice bran is a way of valuing this byproduct, by obtaining an extract with biological potential. The objective of this study was to recover anthocyanins using ultrasound-assisted extraction. Some of the extract was partially purified, and both (crude and partially purified) extracts were evaluated for their anthocyanin content, antioxidant activity, antidiabetic and antitumoral activities, cytotoxicity, and oxidative stress. An increase in the laboratory scale was also achieved, making possible to increase the extraction volume up to 20 times without significantly changing the content of anthocyanins (1.85 mg C3G/g DW). It was found that the purified sample presented a 4.2 times higher value of total anthocyanins compared to the crude sample. The best IC50 values for the purified sample were verified by DPPH and ABTS (0.76 and 0.33 mg/mL). The best results for antidiabetic activity were obtained for the partially purified sample: 0.82 µM C3G for α-glucosidase and 12.5 µM C3G for α-amylase. The extracts demonstrated protection (~70%) when subjected to the oxidative stress of L929 cells. An antitumoral effect of 25–30% for both extracts was found in A459 cells. The crude and partially purified extracts of black rice have antidiabetic and anticancer effects and more studies are needed to explore their potential.

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“…Black rice bran (BRB) was supplied by Ruzene (Pindamonhangaba, Brazil) and prepared according Leonarski et al [5].…”

Section: Sample Preparationmentioning

confidence: 99%

Section: Partial Purification Of Anthocyanin-rich Extractsmentioning

confidence: 99%

Section: Determining the Individual Anthocyanins By Hplc-msmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Potential of Crude and Partially Purified Black Rice Bran Extracts Obtained by Ultrasound-Assisted Extraction: Anti-Glycemic, Cytotoxicity, Cytoprotective, and Antitumoral Effects

Leonarski,

Kuasnei,

Santos

et al. 2024

Foods

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Enhancing anthocyanin extraction efficiency in vegetables and fruits: a high‐speed shear homogenization technology

Ren,

Zhang,

Zhao

et al. 2024

J Sci Food Agric

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BACKGROUNDTo extract anthocyanins with high efficiency, a hypothesis for high‐speed shear homogenization extraction (HSHE) method was established through a combination of solvent and ultrasonic‐assisted extractions. The efficacy of this hypothesis was demonstrated by performing qualitative and quantitative analyses of 16 anthocyanins extracted from five northern vegetables, and five berry fruits using ultra‐high‐performance Q‐Exactive Orbitrap tandem mass spectrometry. Single‐factor experiments were conducted by varying ethanol concentration, temperature, pH and extraction cycles to determine the optimal conditions for this method.RESULTSOptimal extraction conditions (ethanol 70–80%, 40–50 °C, pH 3–4, performed twice) were determined using an HSHE (5 min, 10 000 rpm, 25 °C) assisted shaker (60 min) and ultrasonication (40 kHz, 160 W cm−2, 30 min, 25 °C) procedure. Compared to the traditional non‐HSHE method, the total anthocyanin content obtained through HSHE extraction showed a significant increase, ranging from 1.0 to 3.9 times higher, with purple cabbage exhibiting the most pronounced enhancement in content. More types of anthocyanins were detected in blueberry (9), black bean (7) and raspberry (5), of which malvidin was the major anthocyanin (0.426 g kg−1) in blueberry, having an amount five times than previously obtained.CONCLUSIONThe established HSHE method has been proven to be a superior technique for anthocyanin extraction, with higher extraction efficiency and concentrations. This technique also provides a new avenue for extracting bioactive compounds from diverse food sources, with potential applications in improving the functional properties of food products. © 2024 Society of Chemical Industry.

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Fishwaste Derived Hydroxyapatite Nanostructure Combined with Black Rice Wine for Potential Antioxidant and Antimicrobial Response

Nallasamy,

Muthalagu,

Natarajan

2024

Curr Microbiol

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Pressurized liquid extraction as an eco-friendly approach to recover anthocyanin from black rice bran

Cited by 10 publications

References 58 publications

The Potential of Crude and Partially Purified Black Rice Bran Extracts Obtained by Ultrasound-Assisted Extraction: Anti-Glycemic, Cytotoxicity, Cytoprotective, and Antitumoral Effects

The Potential of Crude and Partially Purified Black Rice Bran Extracts Obtained by Ultrasound-Assisted Extraction: Anti-Glycemic, Cytotoxicity, Cytoprotective, and Antitumoral Effects

Enhancing anthocyanin extraction efficiency in vegetables and fruits: a high‐speed shear homogenization technology

Fishwaste Derived Hydroxyapatite Nanostructure Combined with Black Rice Wine for Potential Antioxidant and Antimicrobial Response

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