Pressurized Propane: An Alternative Technique to Increase Inulinase Activity

Silva, Marceli Fernandes; Golunski, Simone Maria; Rigo, Diane; Mossi, Vinícius; Luccio, Marco Di; Mazutti, Márcio A.; Oliveira, Débora de; Oliveira, J. Vladimir; Treichel, Helen

doi:10.1089/ind.2012.0021

Cited by 8 publications

(3 citation statements)

References 52 publications

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“…The enzymatic activity was determined before (initial activity) and after (final activity) the treatment procedure with pressurized fluids. The experimental condition that led the best results of activity residual were used to pre-treat to enzymes before the FOSs synthesis (Silva et al 2012(Silva et al , 2013a).…”

Section: High-pressure Treatment Of Enzymesmentioning

confidence: 99%

Fructooligosaccharides production in aqueous system using commercial and home-made inulinase after treatment in pressurized fluids

SILVA¹,

RIGO²,

MOSSI³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO-Fructoligosaccharides (FOS) are food additives with several health benefits, especially in the digestive tube, where they act as prebiotics presenting several advantages over other oligosaccharides synthesized industrially. This work is focused on the synthesis of fructooligosaccharides (FOS) from sucrose and inulin using free, immobilized and pre-treated inulinases from Kluyveromyces marxianus NRRL Y 7571 and Aspergillus niger in aqueous system. Initially, the influence of the different gases (propane, n-butane and LPG) on FOS production was investigated. The best results were achieved when both enzymes were previously treated with LPG. For the synthesis of FOS using inulinases from Aspergillus niger, 56.98% of GF2 and 8.47% of GF4, was obtained using inulin as substrate. Using inulinases from Kluyveromyces marxianus NRRL Y 7571, 4.8% of GF2, 5.47% of GF3 and 4.27% of GF4 was achieved, also using inulin as substrate.

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Section: High-pressure Treatment Of Enzymesmentioning

confidence: 99%

Fructooligosaccharides production in aqueous system using commercial and home-made inulinase after treatment in pressurized fluids

SILVA¹,

RIGO²,

MOSSI³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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Section: High-pressure Treatment Of Enzymesmentioning

confidence: 99%

“…To our knowledge, no experimental data about the behavior of inulinases after treatment in compressed fluids are found in the current literature. The present report is part of a broader project and reflects our efforts to help developing new enzyme-catalyzed processes in alternative fluid media (Silva et al, 2012) 2. EXPERIMENTAL…”

Section: Introductionmentioning

confidence: 99%

Compressed N-Butane for treatment of commercial and non-commercial inulinases

Silva¹,

Rigo²,

Mossi³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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210 % in the residual activity using n-butane at 30 bar during 6 h exposure and with a depressurization rate of 20 bar/min. For commercial inulinase from Aspergillus niger, an increment of 142 % in the residual activity at 270 bar for 6 h treatment at the highest depressurization rate (100 bar/min) was observed. The use of compressed n-butane may be of technological relevance as a preceding, preparation step, towards improving enzyme activity, hence helping the development of new biotransformation processes.

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