2017
DOI: 10.1016/j.foodchem.2016.07.053
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Pressurized water extraction of isoflavones by experimental design from soybean flour and Soybean Protein Isolate

Abstract: A Doehlert experimental design was conducted and surface response methodology was used to determine the effect of temperature, contact time and solid liquid ratio on isoflavone extraction from soybean flour or Soybean Protein Isolate in pressurized water system. The optimal conditions conducted gave an extraction yield of 85% from soybean flour. For Soybean Protein Isolate compared to soybean flour, the isoflavone extraction yield is 61%. This difference could be explained by higher aglycon content, while agly… Show more

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Cited by 38 publications
(9 citation statements)
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“…Even at elevated temperatures and moderate pressure, water is not as effective as methanol, ethanol, and their mixtures with water for the isolation of isoflavones [87]. Moreover, the degradation of glucoside and malonyl forms was detected at higher temperature levels during PHWE [178,179].…”
Section: Pressurized Liquid Extraction (Ple)mentioning
confidence: 99%
“…Even at elevated temperatures and moderate pressure, water is not as effective as methanol, ethanol, and their mixtures with water for the isolation of isoflavones [87]. Moreover, the degradation of glucoside and malonyl forms was detected at higher temperature levels during PHWE [178,179].…”
Section: Pressurized Liquid Extraction (Ple)mentioning
confidence: 99%
“…The parameters that influence the efficiency of the extraction are type of solvent, temperature, pressure, sample amount, extraction time, and number of extraction cycles. Several authors have carried out studies on these variables for the extraction of isoflavones from different food matrices (Chang and Chang ; Zgórka ; Delgado‐Zamarreño and others ; Moras and others ). The solvent of choice for extraction of isoflavones is usually aqueous methanol or ethanol in percentages around 80%.…”
Section: Sample Preparationmentioning
confidence: 99%
“…In addition, water can also be used in this technique (PWE), making it a more environmental‐friendly procedure. This technique has recently been employed by Moras and others () who studied the influence of the various operating conditions (temperature, contact time, liquid/solid ratio) on isoflavone extraction from soybean flour or soybean protein isolate (SPI) by pressurized water extraction using an experimental design. The extractions were carried out at a fixed pressure of 100 atm, using sand as the dispersing agent to avoid protein aggregation, and ultrapure water as the solvent.…”
Section: Sample Preparationmentioning
confidence: 99%
“…Extraction alkaline followed by isoelectric precipitation is the methods mostly used to isolate proteins from animal and vegetable protein sources such as milk, soybean, amaranth, quinoa, sesame, sacha inchi, macadamia, and pigeon pea seeds. These technological processes can help to eliminate antinutritional factors [7][8][9][10][11][12][13][14][15][16][17].…”
Section: Introductionmentioning
confidence: 99%