Prestorage inline filtration of whole blood for obtaining white cell‐ reduced blood components

Riggert, Joachim; Schwartz, D. W. M.; Wieding, J. U.; Mayr, W. R.; Köhler, Michael

doi:10.1046/j.1537-2995.1997.371098016442.x

Cited by 41 publications

(41 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This result agrees with recent findings of other authors [20]and diminishes concern about a potential increase in thrombin activity in prestorage WBC–reduced PCs obtained by PRP filtration through negatively charged in–line filters [33, 34]. Moreover, our current data increase controversy on whether there is significant thrombin activation in stored PCs [35, 36, 37, 38]. …”

Section: Discussionsupporting

confidence: 93%

Evaluation of Leukocyte–Depleted Platelet Concentrates Obtained by In–Line Filtration

Ferrer¹,

Rivera²,

Corral³

et al. 2000

Vox Sanguinis

View full text Add to dashboard Cite

Background and Objectives: The use of leukocyte–depleted platelet concentrates (PCs) is justified, yet questions remain regarding their properties. We have assessed the in vitro quality of WBC–reduced PCs obtained by using a new in–line filter. Materials and Methods: Twenty blood units were randomized for standard component preparation, or for processing including in–line leukodepletion of platelet–rich plasma using an ATS^TM PL Pall filter. The resultant PCs were compared during storage for several in vitro platelet quality parameters, content of cytokines and anaphylatoxins, and coagulation markers. Results: In–line filtration of platelet–rich plasma through ATS PL was highly efficient rendering PCs with 99.93% less white blood cells (WBCs) than standard PCs. During storage for up to 9 days, leukocyte–depleted units displayed platelet content, biochemical parameters, and platelet surface expression of glycoproteins Ibα, IIb/IIa, CD62 and CD63, similar to those of standard units. However, in–line leukocyte–reduced PCs displayed no significant accumulation of IL–6 and IL–8 during storage in contrast to standard units. Moreover, while storage promoted complement activation with C3a and C4a liberation in both WBC–reduced PCs and standard units, the concentration of these anaphylatoxins after the 9–day storage period was higher in the former group. Finally, all units, standard and leukocyte–reduced, displayed a similar storage–promoted rise in TGF–β₁, and a mild prolongation of activated partial thromboplastin and prothrombin times. Conclusion: In–line filtration during component preparation appears as an easy and effective procedure for obtaining prestorage leukocyte–depleted PCs. During subsequent blood bank storage, these WBC–reduced PCs display, in comparison with standard PCs, normal in vitro platelet properties, decreased accumulation of cytokines IL–6 and IL–8, and increased complement activation.

show abstract

Section: Discussionsupporting

confidence: 93%

Evaluation of Leukocyte–Depleted Platelet Concentrates Obtained by In–Line Filtration

Ferrer¹,

Rivera²,

Corral³

et al. 2000

Vox Sanguinis

View full text Add to dashboard Cite

show abstract

“…This has led to attempts to remove the leukocytes from blood components. Filtration can be performed both at 4°C (usually most effective) and at 20°C, and prestorage as well as at the bedside [6, 7, 8]. The ability to reduce leukocyte contamination of components through filtration has rapidly improved, and reductions of more than 4 logs are now achievable.…”

Section: The Development Of Blood Component Therapymentioning

confidence: 99%

“…Leukodepletion filters are available for either anticoagulated whole blood, in which case both white cells and platelets are removed, or for red cells and platelet concentrates where only leukocytes are removed [33, 34, 35]. The filtration of whole blood before its separation into red cells and plasma has the advantage of simplicity and high quality of both products.…”

Section: Component Preparationmentioning

confidence: 99%

See 1 more Smart Citation

Liquid–Stored Red Blood Cells for Transfusion

Högman¹

1999

Vox Sanguinis

View full text Add to dashboard Cite

Blood transfusion in a modern sense means the transfusion of red cells, when necessary supplemented by other components. The demand for plasma and plasma fractions and for platelets for therapeutic use has had an influence on the technique for preparing red cells. Automated devices have made it possible to perform collection as well as separation under more standardized conditions. Improved techniques for storage of red cells have prolonged the shelf life somewhat but most of the available methods disregard the rapid loss of 2,3–diphosphoglycerate and the accompanying increase in oxygen affinity. Methods are available which reduce the number of contaminating leukocytes to low levels, but information is still incomplete as to the degree of depletion actually needed.

show abstract

“…Eine Leukozytendepletion vor Lagerung führt bei Vollblut in CPDA-1 zu einer deutlich verbesserten Stabilität der meisten Qualitätsmerkmale der Präparate. Dies gilt insbesondere für Kenngröûen der erythrozytären Viskoelastizität, Flexibilität und dem Mikroaggregatgehalt, aber auch für die klassischen Qualitätsmerkmale ATP-und 2,3-BPG-Konzentration sowie intrazellulärer pH.Bei Erythrozytenkonzentrat in SAG-M führt die Leukozytendepletion zu merklich günstigeren Lagerungsdaten der erythrozytären Viskoelastizität und, in Übereinstimmung mit Literaturangaben[2,20,22,43,47,48], nur zu einer geringfügig verbesserten Konservierung der klassischen Qualitätsmerkmale. In Bezug auf die Flexibilität der Erythrozyten sowie den Mikroaggregatgehalt waren gefilterte und nicht gefilterte Erythrozytenkonzentrate vergleichbar.Bei Vollblut in CPDA-1 führt die Leukozytendepletion zu einer deutlicheren Verbesserung der Lagerungsqualität als bei Erythrozytenkonzentrat in SAG-M. Bei beiden Präparationen wird die Verbesserung vor allem bei den rheologischen Kenngröûen evident.…”

unclassified

Verbesserung der biochemischen und rheologischen Qualität von Vollblut und Erythrozytenkonzentraten durch Leukozytendepletion vor Lagerung

Langer²,

Grossmann³

et al. 2001

Anasthesiol Intensivmed Notfallmed Schmerzthe

View full text Add to dashboard Cite

ERYTHROZYTENQUALIT¾T IN VITRO nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nSchlüsselwörter: Leukozytendepletion ± Vollblut ± Erythrozytenkonzentrat ± Biochemische Lagerungsqualität ± Rheologische LagerungsqualitätKey words: Leucocyte depletion ± Whole blood ± Red cell concentrate ± Biochemical storage lesion ± Rheological storage lesion Einleitung Während im homologen Bereich eine Auftrennung der Vollblutspenden (VB) in Buffy coat-freies Erythrozytenkonzentrat (EK) und gefrorenes Frischplasma (GFP) als transfusionsmedizinischer Standard gilt, kann Eigenblut auch in Form von leukozytendepletiertem VB gelagert werden [44]. Gegen eine Auftrennung des Eigenbluts in Komponenten spricht, dass sich die Indikation zur Gabe von GFP in der Regel erst mit einem Blutverlust von mehr als 2/3 des zirkulierenden Blutvolumens entwickelt [33] und die meisten elektiven Eingriffe deshalb zu keinem entsprechenden Bedarf führen. Infolgedessen wird ein Groûteil des autologen GFP verworfen oder allenfalls als Volumenersatz verwendet [30, 36]. Als einziger Nachteil von leukozytendepletiertem VB verbleibt somit seine kürzere Haltbarkeit von derzeit maximal 35 Tagen (EK: 42 ± 49 Tage) [44]. Dem stehen jedoch erhebliche logistische, technische und finanzielle Vorteile gegenüber. In den vergangenen Jahren entwickelte sich deshalb eine kontroverse Diskussion über die ¹Eigenblutkonserve der Wahlª [26 ± 28, 34, 60, 61].

show abstract

Prestorage inline filtration of whole blood for obtaining white cell‐ reduced blood components

Abstract: Blood components prepared from inline-filtered whole blood meet the standards for WBC-reduced RBCs and FFP. The protein profile of FFP-F is not altered, and markers for the activation of coagulation and fibrinolysis show no increase.

Cited by 41 publications

References 17 publications

Evaluation of Leukocyte–Depleted Platelet Concentrates Obtained by In–Line Filtration

Evaluation of Leukocyte–Depleted Platelet Concentrates Obtained by In–Line Filtration

Liquid–Stored Red Blood Cells for Transfusion

Verbesserung der biochemischen und rheologischen Qualität von Vollblut und Erythrozytenkonzentraten durch Leukozytendepletion vor Lagerung

Contact Info

Product

Resources

About

Prestorage inline filtration of whole blood for obtaining white cell‐ reduced blood components

Abstract: Blood components prepared from inline-filtered whole blood meet the standards for WBC-reduced RBCs and FFP. The protein profile of FFP-F is not altered, and markers for the activation of coagulation and fibrinolysis show no increase.

Cited by 41 publications

References 17 publications

Evaluation of Leukocyte&ndash;Depleted Platelet Concentrates Obtained by In&ndash;Line Filtration

Evaluation of Leukocyte&ndash;Depleted Platelet Concentrates Obtained by In&ndash;Line Filtration

Liquid&ndash;Stored Red Blood Cells for Transfusion

Verbesserung der biochemischen und rheologischen Qualität von Vollblut und Erythrozytenkonzentraten durch Leukozytendepletion vor Lagerung

Contact Info

Product

Resources

About

Evaluation of Leukocyte–Depleted Platelet Concentrates Obtained by In–Line Filtration

Evaluation of Leukocyte–Depleted Platelet Concentrates Obtained by In–Line Filtration

Liquid–Stored Red Blood Cells for Transfusion