Prestorage Leucofiltration of Red Blood Cell Suspensions Modulates Storage Time-Dependent Suppression of in vitro Cytokine Release

Mynster, Tommie; Nielsen, Hans J.

doi:10.1159/000025286

Cited by 3 publications

(1 citation statement)

References 34 publications

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“…However, even though the reduction of leukocyte content in blood products by prestorage leukodepletion seems to be a reasonable approach to preventing TRIM, doubts have been raised against the universal implementation of leukocyte reduction. This is because immunomodulatory effects have been described even after transfusion of leukocyte-depleted blood [ 58 , 59 ]. So, it has been argued that the remaining immunomodulatory effect of blood transfusion, even after prestorage leukodepletion, could be mediated either by the few remaining leukocytes or by unidentified bioactive substances other than those present in leukocytes.…”

Section: Discussionmentioning

confidence: 99%

The Impact of Two Different Transfusion Strategies on Patient Immune Response during Major Abdominal Surgery: A Preliminary Report

Theodoraki

Markatou

Rizos

et al. 2014

Journal of Immunology Research

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Blood transfusion is associated with well-known risks. We investigated the difference between a restrictive versus a liberal transfusion strategy on the immune response, as expressed by the production of inflammatory mediators, in patients subjected to major abdominal surgery procedures. Fifty-eight patients undergoing major abdominal surgery were randomized preoperatively to either a restrictive transfusion protocol or a liberal transfusion protocol (with transfusion if hemoglobin dropped below 7.7 g dL−1 or 9.9 g dL−1, respectively). In a subgroup of 20 patients randomly selected from the original allocation groups, blood was sampled for measurement of IL-6, IL-10, and TNFα. Postoperative levels of IL-10 were higher in the liberal transfusion group on the first postoperative day (49.82 ± 29.07 vs. 15.83 ± 13.22 pg mL−1, P < 0.05). Peak postoperative IL-10 levels correlated with the units of blood transfused as well as the mean duration of storage and the storage time of the oldest unit transfused (r 2 = 0.38, P = 0.032, r 2 = 0.52, P = 0.007, and r 2 = 0.68, P<0.001, respectively). IL-10 levels were elevated in patients with a more liberal red blood cell transfusion strategy. The strength of the association between anti-inflammatory IL-10 and transfusion variables indicates that IL-10 may be an important factor in transfusion-associated immunomodulation. This trial is registered under ClinicalTrials.gov Identifier: NCT02020525.

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Section: Discussionmentioning

confidence: 99%

The Impact of Two Different Transfusion Strategies on Patient Immune Response during Major Abdominal Surgery: A Preliminary Report

Theodoraki

Markatou

Rizos

et al. 2014

Journal of Immunology Research

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Impact of Alloantigens and Storage-associated Factors on Stimulated Cytokine Response in an In Vitro Model of Blood Transfusion

Biedler¹,

Schneider²,

Seyfert

et al. 2002

Anesthesiology

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These data suggest that banked whole blood has an immunosuppressive effect that is largely attributable to storage-dependent factors. These factors are partially removed by prestorage leukodepletion, while the contribution of alloantigens is of minor significance. Immunosuppressive effects are least apparent with leukodepleted erythrocytes, suggesting that the presence of plasma during storage is required for the immunosuppressive effect to develop.

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Induction of efficient apoptosis and cell‐cycle arrest in tumor cells by adenovirus‐mediated p53 A4 mutant

Maeda

Nakamura²,

Isono

et al. 2006

Pathology International

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p53 is an effective tumor suppressor and is inactivated in numerous cancer cells. In the present study, p53 mutant A4, which carries mutations in C-terminus of the protein and is resistant to murine double minute 2-mediated degradation, was exploited to introduce p53 function in tumor cells. The effect of p53 A4 mutant with recombinant adenovirus vector (Ad-p53 A4) was examined. Ad-p53 A4 infection at a low multiplicity of infection showed significant accumulation of p53 protein and strongly induced a killing effect on osteosarcoma cell line MG-63 that is less sensitive to transduction of wild-type p53. DNA fragmentation assay and caspase assay showed that the cell death induced by Ad-p53 A4 was more rapid and higher than that by Ad-p53 wild-type infection. It is also showed Ad-p53 A4 induces cell-cycle arrest in G1 phase. Moreover, a similar effect was observed in some human cancer cell lines (HeLa, HepG2, KATO III and Saos-2) in various status of endogenous p53 expression. These results suggest that Ad-p53 A4 has the ability to strongly suppress tumor cells and is a promising, novel tool for cancer gene therapy.

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Prestorage Leucofiltration of Red Blood Cell Suspensions Modulates Storage Time-Dependent Suppression of in vitro Cytokine Release

Cited by 3 publications

References 34 publications

The Impact of Two Different Transfusion Strategies on Patient Immune Response during Major Abdominal Surgery: A Preliminary Report

The Impact of Two Different Transfusion Strategies on Patient Immune Response during Major Abdominal Surgery: A Preliminary Report

Impact of Alloantigens and Storage-associated Factors on Stimulated Cytokine Response in an In Vitro Model of Blood Transfusion

Induction of efficient apoptosis and cell‐cycle arrest in tumor cells by adenovirus‐mediated p53 A4 mutant

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