Prestorage white cell reduction in saline‐adenine‐glucose‐mannitol red cells by use of an integral filter: evaluation of storage values and invivo recovery

Rogers, S. E.; Edmondson, Daryl L.; Goodrick, M. J.; Standen, Graham R.; Franck, Valentine; Reppucci, A.J.; Pamphilon, D. H.

doi:10.1046/j.1537-2995.1995.35996029155.x

Cited by 19 publications

(20 citation statements)

References 27 publications

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“…5 leucodepletion and is comparable with data published for other third-generation filtration systems (Heaton et al, 1994;Riggert et al, 1995;Rogers et al, 1995). Indeed, our work supports previous studies which showed significantly less haemolysis and potassium leakage in leucodepleted units (Heaton et al, 1994;Rogers et al, 1995). This may be attributed to several factors including temperature/time induced alterations in the adhesive capacity and deformability of blood cells as well as leucocyte fragmentation (Steneker et al, 1995).…”

Section: Discussionsupporting

confidence: 91%

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Evaluation of a new, integral, whole blood filter (RS2000) system for prestorage leucodepletion of SAG‐M red cells

et al. 1996

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Residual donor leucocytes are responsible for many adverse transfusion reactions. Prestorage leucodepletion may ameliorate these effects and enhance product quality. We studied a bottom and top (BAT) system incorporating an integral filter for whole blood leucodepletion. Our evaluation assessed leucodepletion efficiency as well as in vitro SAG-M red cell quality and storage characteristics. Sixty-six units of blood were collected; test units into the Optipac-pLuS system and controls into the standard triple pack configuration. Test units were held for 4-6 h at room temperature (rt) or 12-18 h at 4 degrees C. The mean leucocyte counts for the SAG-M red cells in the quality and storage trial were 0.6 x 10(6) (rt hold), 0.05 x 10(6) (4 degrees C hold) and 2500 x 10(6) (controls). We observed no significant differences between the groups for Na+, ATP, 2,3-DPG, glucose, lactate and pH during the 49 d storage. The control group, however, showed a greater increase in haemolysis and K+ with time. Autologous in vivo 24 h red cell recovery, after 42 d storage, was > 75%. Adjustment of processing parameters in subsequent studies gave leucodepleted SAG-M red cells with minimal cell loss (9.19%) plus acceptable haemoglobin content (46-76 g/U) and haematocrit (54-62%). This system achieved > 3.5 log leucodepletion with all but one unit containing < 1 x 10(6) leucocytes. The product quality is good and the system suitable for routine use in blood centres.

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Section: Discussionsupporting

confidence: 91%

“…Previous studies (Rogers et al, 1995) provide equivalent data for buffy coat depleted (1 log) products prepared in the Optipac 1 configuration. Component separation was performed on Optipress 1 automated blood component extractors (Baxter) according to the manufacturer's instructions.…”

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confidence: 99%

Evaluation of a new, integral, whole blood filter (RS2000) system for prestorage leucodepletion of SAG‐M red cells

et al. 1996

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“…Even though hemolysis rates increased over the 42‐day period, they were still well below the limit of 0.8 percent that was suggested by the international standards 17 . After 42 days, analysis of the remaining storage variables, such as pH, supernatant potassium, ATP/Hb, and 2,3 DPG, revealed results similar to previously published data 12 and our own observations in buffy coat‐depleted RBCs 10 . Thus, we found no detrimental effects of prestorage WBC filtration on RBC storage.…”

Section: Discussionsupporting

confidence: 90%

Prestorage WBC filtration of RBC units with soft‐shell filters: filtration performance and impact on RBCs during storage for 42 days

et al. 2002

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WBC filtration with two newly developed soft-shell filters showed acceptable WBC-reduction efficacy without any difference between filter types in buffy coat-depleted RBCs from 450- and 500-mL whole-blood donations. However, the application of both filters resulted in an unacceptably low RBC recovery after filtration, which was particularly evident with the LCR5 filter. Our findings raise concern that WBC reduction with these filters may result in the production of RBCs with an inappropriately low Hb concentration.

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“…It has also been described by various authors that the presence of leukocytes in the red cell units contribute significantly to an increase in red cell hemolysis during storage,[71011] and that prestorage filtration helps in reducing the extent of this hemolysis. [1314] This is due to the release of various chemicals and enzymes, especially proteases from the leukocytes. For the same reason, various leukocytes reduction filters are commercially available.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the red cell hemolysis in packed red cells during processing and storage

Makroo¹,

Raina²,

Bhatia³

et al. 2011

Asian J Transfus Sci

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Introduction:Storage of red cells causes a progressive increase in hemolysis. In spite of the use of additive solutions for storage and filters for leucoreduction, some amount of hemolysis is still inevitable. The extent of hemolysis, however, should not exceed the permissible threshold for hemolysis even on the 42nd day of storage.Study Design and Methods:Eighty units of packed red cells, 40 stored in SAGM post leucoreduction and 40 in ADSOL without leucoreduction filters, were evaluated for plasma hemoglobin by HemoCue Plasma Hemoglobin analyzer on the day of collection and on the 7th, 14th, 21st, 28th, 35th and 42nd days thereafter. The hemoglobin and hematocrit were also noted for all these units by the Beckman and Coulter analyzer. Percentage hemolysis was then calculated.Observations:Hemolysis progressively increased with the storage period in all the stored red cell units (SAGM as well ADSOL). However, on day 42nd of storage, free hemoglobin in all the red cell units was within the permissible level (which is 0.8% according to the Council of Europe guidelines and 1% as per the US FDA guidelines). The mean percentage hemolysis was slightly higher in the SAGM-containing bags with an integral leucoreduction filter as compared to the bags containing ADSOL. However this difference was marginal and not statistically significant.Conclusion:Hemolysis of the red cells increases with storage. However, maximum hemolysis does not exceed the permissible limits at any time thereby indicating the effect of optimum processing and storage conditions on red cell hemolysis.

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Prestorage white cell reduction in saline‐adenine‐glucose‐mannitol red cells by use of an integral filter: evaluation of storage values and invivo recovery

Abstract: The combination of buffy coat removal and filtration in the blood container system with an integral filter achieved effective WBC reduction (> or = 3 log10 reduction from whole blood) without biologically significant detriment to in vitro or in vivo storage values.

Cited by 19 publications

References 27 publications

Evaluation of a new, integral, whole blood filter (RS2000) system for prestorage leucodepletion of SAG‐M red cells

Evaluation of a new, integral, whole blood filter (RS2000) system for prestorage leucodepletion of SAG‐M red cells

Prestorage WBC filtration of RBC units with soft‐shell filters: filtration performance and impact on RBCs during storage for 42 days

Evaluation of the red cell hemolysis in packed red cells during processing and storage

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