Presumed and Actual Concentrations of Reduced Glutathione in Preservation Solutions

Breussegem, A Van; Pelt, Jos van; Wylin, Tine; Heedfeld, Veerle; Zeegers, Marcel; Monbaliu, Diethard; Pirenne, Jacques; Vekemans, Katrien

doi:10.1016/j.transproceed.2011.09.031

Cited by 7 publications

(8 citation statements)

References 13 publications

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“…Reduced glutathione (GSH) was measured in the liver extracts using the procedure previously described [ 28 ]. Liver samples were homogenized in cold buffer containing 5 mm phosphate–EDTA buffer (pH 8.0) and 25% HPO3.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…In this communication, we demonstrated for the first time how the direct effects of PEG35 on mitochondrial ALDH2 contribute towards maintaining mitochondrial functionality during ischemia preservation. Mitochondrial ALDH2 could act as a gatekeeper of ROS overproduction protecting the liver graft from the damaging effects of transient aldehydes produced [22][23][24] besides other additives in preservation solutions such as labile glutathione [28] that play an important role against oxidative stress. The results reported here reveal the superior antioxidant capacity of IGL-2 (due to ALDH2 combined with increased glutathione content) against the ischemic insult during graft preservation, presenting an interesting tool to be considered for improving hypothermic fatty liver preservation by using static and dynamic approaches.…”

Section: Introductionmentioning

confidence: 99%

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Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach

Bardallo

Silva

Carbonell

et al. 2021

IJMS

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The total damage inflicted on the liver before transplantation is associated with several surgical manipulations, such as organ recovery, washout of the graft, cold conservation in organ preservation solutions (UW, Celsior, HTK, IGL-1), and rinsing of the organ before implantation. Polyethylene glycol 35 (PEG35) is the oncotic agent present in the IGL-1 solution, which is an alternative to UW and Celsior solutions in liver clinical transplantation. In a model of cold preservation in rats (4 °C; 24 h), we evaluated the effects induced by PEG35 on detoxifying enzymes and nitric oxide, comparing IGL-1 to IGL-0 (which is the same as IGL-1 without PEG). The benefits were also assessed in a new IGL-2 solution characterized by increased concentrations of PEG35 (from 1 g/L to 5 g/L) and glutathione (from 3 mmol/L to 9 mmol/L) compared to IGL-1. We demonstrated that PEG35 promoted the mitochondrial enzyme ALDH2, and in combination with glutathione, prevented the formation of toxic aldehyde adducts (measured as 4-hydroxynonenal) and oxidized proteins (AOPP). In addition, PEG35 promoted the vasodilator factor nitric oxide, which may improve the microcirculatory disturbances in steatotic grafts during preservation and revascularization. All of these results lead to a reduction in damage inflicted on the fatty liver graft during the cold storage preservation. In this communication, we report on the benefits of IGL-2 in hypothermic static preservation, which has already been proved to confer benefits in hypothermic oxygenated dynamic preservation. Hence, the data reported here reinforce the fact that IGL-2 is a suitable alternative to be used as a unique solution/perfusate when hypothermic static and preservation strategies are used, either separately or combined, easing the logistics and avoiding the mixture of different solutions/perfusates, especially when fatty liver grafts are used. Further research regarding new therapeutic and pharmacological insights is needed to explore the underlying mitochondrial mechanisms exerted by PEG35 in static and dynamic graft preservation strategies for clinical liver transplantation purposes.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach

Bardallo

Silva

Carbonell

et al. 2021

IJMS

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“…Glutathione is present in its reduced form and is another critical component often used in preservation solutions 22 . Its antioxidant properties are essential for organ preservation, especially in preparing the graft during reperfusion.…”

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confidence: 99%

Original and generic preservation solutions in organ transplantation. A new paradigm?

Roselló‐Catafau

Panisello‐Roselló

Pasut³

et al. 2020

Acta Cir. Bras.

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Solid organ transplantation is a very complex process, in which the storage of the graft in a preservation solution is mandatory in order to extend ischemic times and contain further damage. The condition in which the organ is transplanted is critical for the outcome of the organ recipient. The recent emergence of generic versions of organ preservation solutions (solutions with the same composition and under the same legislation as the original versions, but with different brands) compelled us to study whether the standards are maintained when comparing the original and its generic counterpart. Along these lines, we discuss and comment on some aspects concerning this issue of general interest in the organ transplantation field.

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“…In any case, the induction of “hyperthermia strategies” during organ perfusion in hypothermic and subnormothermic conditions should be consistent with the subsequent increases in HSP expression (which usually needs longer time after heat exposure), and it would need to be evaluated. In addition, it is important to remark that there are some limitations of HOPE strategies associated with perfusion solutions containing glutathione, such as KPS , given that it is easily oxidized . These limitations could be even more exacerbated when subnormothermic conditions (20°C) are used.…”

mentioning

confidence: 99%

New trends in transient hyperthermia and liver preservation

Roselló‐Catafau

2020

Transpl Int

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Presumed and Actual Concentrations of Reduced Glutathione in Preservation Solutions

Cited by 7 publications

References 13 publications

Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach

Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach

Original and generic preservation solutions in organ transplantation. A new paradigm?

New trends in transient hyperthermia and liver preservation

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